Abstract
Colonic epithelia from guinea-pigs, sensitized by feeding with cow milk, responded to antigen (beta-lactoglobulin) challenge when applied to the serosal, but not the mucosal, side of the tissue. The response, under short circuit conditions, was an inwardly directed current due to chloride secretion. Two detergents, deoxycholate and Triton X-100, caused the basal short circuit current to decrease and transepithelial conductance to increase when applied to the mucosal surface. After removing detergents from the bathing solution tissues now responded to antigen challenge from the mucosal side, without impairment of the overall response. There was a correlation between the conductance change induced by detergents and the fraction of the total response which could be elicited form the mucosal side of the tissue. It was concluded that models of local hypersensitivity reactions to ingested foodstuffs require both development of immunological sensitivity plus increased permeability to antigen. The role of bile salts in inducing the latter is discussed.
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Selected References
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- Al-Dhahir H. A., Zeitlin I. J. Bile salts activate glandular kallikrein-like activity in rat colon. Adv Exp Med Biol. 1983;156:463–467. [PubMed] [Google Scholar]
- Binder H. J., Dobbins J. W., Racusen L. C., Whiting D. S. Effect of propranolol on ricinoleic acid- and deoxycholic acid-induced changes of intestinal electrolyte movement and mucosal permeability. Evidence against the importance of altered permeability in the production of fluid and electrolyte accumulation. Gastroenterology. 1978 Oct;75(4):668–673. [PubMed] [Google Scholar]
- Binder H. J., Filburn C., Volpe B. T. Bile salt alteration of colonic electrolyte transport: role of cyclic adenosine monophosphate. Gastroenterology. 1975 Mar;68(3):503–508. [PubMed] [Google Scholar]
- Coombs R. R., Devey M. E., Anderson K. J. Refractoriness to anaphylactic shock after continuous feeding of cow's milk to guinea-pigs. Clin Exp Immunol. 1978 May;32(2):263–271. [PMC free article] [PubMed] [Google Scholar]
- Cuthbert A. W., Margolius H. S. Kinins stimulate net chloride secretion by the rat colon. Br J Pharmacol. 1982 Apr;75(4):587–598. doi: 10.1111/j.1476-5381.1982.tb09178.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cuthbert A. W., McLaughlan P., Coombs R. R. Immediate hypersensitivity reaction to beta-lactoglobulin in the epithelium lining the colon of guinea pigs fed cows' milk. Int Arch Allergy Appl Immunol. 1983;72(1):34–40. doi: 10.1159/000234837. [DOI] [PubMed] [Google Scholar]
- Hofmann A. F. The syndrome of ileal disease and the broken enterohepatic circulation: cholerheic enteropathy. Gastroenterology. 1967 Apr;52(4):752–757. [PubMed] [Google Scholar]
- Kessel D., Cuthbert A. W. Sidedness of the reaction to beta-lactoglobulin in sensitised colonic epithelia. Int Arch Allergy Appl Immunol. 1984;74(2):113–119. doi: 10.1159/000233530. [DOI] [PubMed] [Google Scholar]
- Mekjian H. S., Phillips S. F., Hofmann A. F. Colonic secretion of water and electrolytes induced by bile acids: perfusion studies in man. J Clin Invest. 1971 Aug;50(8):1569–1577. doi: 10.1172/JCI106644. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saunders D. R., Hedges J. R., Sillery J., Esther L., Matsumura K., Rubin C. E. Morphological and functional effects of bile salts on rat colon. Gastroenterology. 1975 May;68(5 Pt 1):1236–1245. [PubMed] [Google Scholar]