Abstract
The slow component of deactivation of guinea-pig isolated ileum to C5adesArg was studied to analyse the mechanism of loss and subsequent recovery of sensitivity. Neither cycloheximide (10(-3) M) nor colchicine (5 X 10(-5) M), vinblastine, lumicolchicine, or cytochalasin B (each 2 X 10(-5) M) affected significantly the spasmogenic effect of C5adesArg or the course of deactivation produced by repeated applications; chloroquine (2 X 10(-4) M) inhibited the spasmogenic effect unspecifically without interfering with deactivation. Recovery from slow deactivation was totally blocked by chloroquine and considerably diminished by colchicine and vinblastine, but was not affected by the other agents. It is proposed that recovery involves lysosomal processing of C5a receptors (occupied by the peptide) but does not require biosynthesis of new receptors.
Full text
PDF






Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chenoweth D. E., Goodman M. G. The C5a receptor of neutrophils and macrophages. Agents Actions Suppl. 1983;12:252–273. doi: 10.1007/978-3-0348-9352-7_15. [DOI] [PubMed] [Google Scholar]
- Chenoweth D. E., Goodman M. G., Weigle W. O. Demonstration of a specific receptor for human C5a anaphylatoxin on murine macrophages. J Exp Med. 1982 Jul 1;156(1):68–78. doi: 10.1084/jem.156.1.68. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ciechanover A., Schwartz A. L., Dautry-Varsat A., Lodish H. F. Kinetics of internalization and recycling of transferrin and the transferrin receptor in a human hepatoma cell line. Effect of lysosomotropic agents. J Biol Chem. 1983 Aug 25;258(16):9681–9689. [PubMed] [Google Scholar]
- Dise C. A., Burch J. W., Goodman D. B. Direct interaction of mepacrine with erythrocyte and platelet membrane phospholipid. J Biol Chem. 1982 May 10;257(9):4701–4704. [PubMed] [Google Scholar]
- Famaey J., Fontaine J., Reuse J. An analysis of the inhibitory effects and of possible prostaglandins antagonism of chloroquine in the guinea-pig isolated ileum. J Pharm Pharmacol. 1977 Dec;29(12):761–762. doi: 10.1111/j.2042-7158.1977.tb11457.x. [DOI] [PubMed] [Google Scholar]
- Flanagan M. D., Lin S. Cytochalasins block actin filament elongation by binding to high affinity sites associated with F-actin. J Biol Chem. 1980 Feb 10;255(3):835–838. [PubMed] [Google Scholar]
- Gonzalez-Noriega A., Grubb J. H., Talkad V., Sly W. S. Chloroquine inhibits lysosomal enzyme pinocytosis and enhances lysosomal enzyme secretion by impairing receptor recycling. J Cell Biol. 1980 Jun;85(3):839–852. doi: 10.1083/jcb.85.3.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grant J. A., Dupree E., Thueson D. O. Complement-mediated release of histamine from human basophils. III. Possible regulatory role of microtubules and microfilaments. J Allergy Clin Immunol. 1977 Nov;60(5):306–311. doi: 10.1016/0091-6749(77)90110-5. [DOI] [PubMed] [Google Scholar]
- Hook W. A., Siraganian R. P. Complement-induced histamine release from human basophils. III. Effect of pharmacologic agents. J Immunol. 1977 Feb;118(2):679–684. [PubMed] [Google Scholar]
- King A. C., Hernaez-Davis L., Cuatrecasas P. Lysomotropic amines cause intracellular accumulation of receptors for epidermal growth factor. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3283–3287. doi: 10.1073/pnas.77.6.3283. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lüllmann H., Plösch H., Ziegler A. Ca replacement by cationic amphiphilic drugs from lipid monolayers. Biochem Pharmacol. 1980 Nov 1;29(21):2969–2974. doi: 10.1016/0006-2952(80)90046-5. [DOI] [PubMed] [Google Scholar]
- Manku M. S., Horrobin D. F. Chloroquine, quinine, procaine, quinidine and clomipramine are prostaglandin agonists and antagonists. Prostaglandins. 1976 Nov;12(5):789–801. doi: 10.1016/0090-6980(76)90053-8. [DOI] [PubMed] [Google Scholar]
- Mego J. L., Chung C. H. Effects of some antimalarials and related substances on intralysosomal proteolysis. Biochem Pharmacol. 1979;28(4):465–470. doi: 10.1016/0006-2952(79)90237-5. [DOI] [PubMed] [Google Scholar]
- Ohkuma S., Poole B. Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3327–3331. doi: 10.1073/pnas.75.7.3327. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sullivan S. J., Zigmond S. H. Chemotactic peptide receptor modulation in polymorphonuclear leukocytes. J Cell Biol. 1980 Jun;85(3):703–711. doi: 10.1083/jcb.85.3.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Szego C. M., Pietras R. J. Lysosomal functions in cellular activation: propagation of the actions of hormones and other effectors. Int Rev Cytol. 1984;88:1–302. doi: 10.1016/s0074-7696(08)62759-x. [DOI] [PubMed] [Google Scholar]
- Tauber A. I., Simons E. R. Dissociation of human neutrophil membrane depolarization, respiratory burst stimulation and phospholipid metabolism by quinacrine. FEBS Lett. 1983 May 30;156(1):161–164. doi: 10.1016/0014-5793(83)80269-5. [DOI] [PubMed] [Google Scholar]
- Whittaker J., Hammond V. A., Alberti K. G. Effects of colchicine on insulin binding to isolated rat hepatocytes. Biochem Biophys Res Commun. 1981 Dec 15;103(3):1100–1106. doi: 10.1016/0006-291x(81)90921-9. [DOI] [PubMed] [Google Scholar]
- Wibo M., Poole B. Protein degradation in cultured cells. II. The uptake of chloroquine by rat fibroblasts and the inhibition of cellular protein degradation and cathepsin B1. J Cell Biol. 1974 Nov;63(2 Pt 1):430–440. doi: 10.1083/jcb.63.2.430. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zigmond S. H., Sullivan S. J., Lauffenburger D. A. Kinetic analysis of chemotactic peptide receptor modulation. J Cell Biol. 1982 Jan;92(1):34–43. doi: 10.1083/jcb.92.1.34. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zimmermann B., Damerau B., Vogt W. Purification and partial amino acid sequence of classical anaphylatoxin from pig serum: identification with Des-Arg-C5a. Hoppe Seylers Z Physiol Chem. 1980;361(6):915–924. doi: 10.1515/bchm2.1980.361.1.915. [DOI] [PubMed] [Google Scholar]
- de Duve C., de Barsy T., Poole B., Trouet A., Tulkens P., Van Hoof F. Commentary. Lysosomotropic agents. Biochem Pharmacol. 1974 Sep 15;23(18):2495–2531. doi: 10.1016/0006-2952(74)90174-9. [DOI] [PubMed] [Google Scholar]