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. 1984 May;82(1):217–222. doi: 10.1111/j.1476-5381.1984.tb16461.x

Substance P in sensory nerve fibres contributes to the development of oedema in the rat hind paw after thermal injury.

A Saria
PMCID: PMC1987261  PMID: 6203590

Abstract

Immersion of the hind paws of anesthetized rats in hot water for 5 min induced massive plasma protein leakage as indicated by extravasation of Evans blue dye in the skin. The threshold temperature which caused noticeable plasma extravasation was 45 degrees C, a maximal response was obtained between 55 degrees C and 60 degrees C. Pretreatment of rats 2 days after birth with 50 mg kg-1 capsaicin significantly reduced the Evans blue extravasation induced by hot water at 50 degrees C and 60 degrees C, whereas guanethidine pretreatment 24 h before the experiment caused a significantly increased response at 40 degrees C, 45 degrees C and 50 degrees C. When Evans blue was injected between 10 and 120 min after immersion of the paw in hot water, a significant extravasation of the dye was no longer detectable. However, the weight of the paw as well as the weight of the piece of skin taken for Evans blue quantification increased during this period indicating the progressive development of oedema in the skin and underlying tissues. In rats treated with capsaicin as neonates, the increase in paw weight after immersion in water of 50 degrees C for 5 min was significantly delayed during the first hour, but there was no difference after two hours. In rats pretreated with D-Arg1,D-Pro2-,D- Trp7 ,9, Leu11 -substance P, a substance P (SP) antagonist, the Evans blue extravasation was significantly reduced. However, the response, which remained in rats treated with capsaicin as neonates was not blocked by the SP-antagonist.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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