Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1985 Feb;84(2):299–308. doi: 10.1111/j.1476-5381.1985.tb12914.x

Ion dependence of the release of noradrenaline by tetraethylammonium and 4-aminopyridine from cat splenic slices.

V Ceña, A G García, C Gonzalez-Garcia, S M Kirpekar
PMCID: PMC1987285  PMID: 2983808

Abstract

Cat splenic slices prelabelled with [3H]-noradrenaline were incubated in oxygenated Krebs-bicarbonate solution at 37 degrees C, and the spontaneous total 3H release into different incubation media monitored. In normal Krebs bicarbonate solution, the spontaneous tritium fractional release amounted to 3.7% of the tissue radioactivity content per 5 min collection period. Tetraethylammonium (TEA) increased spontaneous transmitter release in a concentration-dependent manner; the release was maximal at 30 mM and was 3.5 times the basal release. 4-Aminopyridine (4-AP) also enhanced the spontaneous release of tritium. The response increased linearly with 4-AP concentration (1-10 mM). With 10 mM 4-AP, the release was as much as 6 times the basal transmitter release. Guanidine was much less potent than either TEA or 4-AP. The secretory response to TEA or 4-AP was little affected by changes in external Ca2+, Mg2+, Na+, Cl-, H2PO4- or by tetrodotoxin. However, transmitter release evoked by TEA or 4-AP strongly depended upon the concentration of HCO3- of the incubation solution; in fact, the secretory response varied almost linearly between 1 and 25 mM HCO3-. The mechanisms underlying these effects are probably related to the well-known ability of TEA and 4-AP to block K+ conductance that would cause depolarization of the splenic sympathetic nerve terminals. The HCO3- requirements for the secretory response are probably related to the ability of CO2/HCO3- solutions to mobilize and release Ca2+ from intracellular organelles.

Full text

PDF
299

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bowman W. C., Savage A. O. Pharmacological actions of aminopyridines and related compounds. Rev Pure Appl Pharmacol Sci. 1981 Oct-Dec;2(4):317–371. [PubMed] [Google Scholar]
  2. Cabantchik Z. I., Rothstein A. The nature of the membrane sites controlling anion permeability of human red blood cells as determined by studies with disulfonic stilbene derivatives. J Membr Biol. 1972 Dec 29;10(3):311–330. doi: 10.1007/BF01867863. [DOI] [PubMed] [Google Scholar]
  3. Douglas W. W., Rubin R. P. The mechanism of catecholamine release from the adrenal medulla and the role of calcium in stimulus-secretion coupling. J Physiol. 1963 Jul;167(2):288–310. doi: 10.1113/jphysiol.1963.sp007150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Esquerro E., Schiavone M., García A. G., Kirpekar S. M., Prat J. C. Release of endogenous noradrenaline from ligated cat hypogastric nerve by veratridine. Eur J Pharmacol. 1980 Sep 5;66(4):367–373. doi: 10.1016/0014-2999(80)90469-0. [DOI] [PubMed] [Google Scholar]
  5. FRANKENHAEUSER B., HODGKIN A. L. The action of calcium on the electrical properties of squid axons. J Physiol. 1957 Jul 11;137(2):218–244. doi: 10.1113/jphysiol.1957.sp005808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FRANKENHAEUSER B., MEVES H. The effect of magnesium and calcium on the frog myelinated nerve fibre. J Physiol. 1958 Jul 14;142(2):360–365. doi: 10.1113/jphysiol.1958.sp006022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Garcia A. G., Hernandez M., Horga J. F., Sanchez-Garcia P. On the release of catecholamines and dopamine-beta-hydroxylase evoked by ouabain in the perfused cat adrenal gland. Br J Pharmacol. 1980 Mar;68(3):571–583. doi: 10.1111/j.1476-5381.1980.tb14573.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hirsch J., Kirpekar S. M., Prat J. C. Effect of guanidine on release of noradrenaline from the perfused spleen of the cat. Br J Pharmacol. 1979 Aug;66(4):537–546. doi: 10.1111/j.1476-5381.1979.tb13692.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kasbekar D. K., Durbin R. P. An adenosine triphosphatase from frog gastric mucosa. Biochim Biophys Acta. 1965 Sep 20;105(3):472–482. doi: 10.1016/s0926-6593(65)80232-6. [DOI] [PubMed] [Google Scholar]
  10. Katz B., Miledi R. Spontaneous and evoked activity of motor nerve endings in calcium Ringer. J Physiol. 1969 Aug;203(3):689–706. doi: 10.1113/jphysiol.1969.sp008887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kirpekar M., Kirpekar S. M., Prat J. C. Effect of 4-aminopyridine on release of noradrenaline from the perfused cat spleen by nerve stimulation. J Physiol. 1977 Nov;272(3):517–528. doi: 10.1113/jphysiol.1977.sp012057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kirpekar S. M., Garcia A. G., Prat J. C. Action of nicotine on sympathetic nerve terminals. J Pharmacol Exp Ther. 1980 Apr;213(1):133–138. [PubMed] [Google Scholar]
  13. Kirpekar S. M., Misu Y. Release of noradrenaline by splenic nerve stimulation and its dependence on calcium. J Physiol. 1967 Jan;188(2):219–234. doi: 10.1113/jphysiol.1967.sp008135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kirpekar S. M., Prat J. C. Effect of tetraethylammonium on noradrenaline release from cat spleen treated with tetrodotoxin. Nature. 1978 Dec 7;276(5688):623–624. doi: 10.1038/276623a0. [DOI] [PubMed] [Google Scholar]
  15. Kirpekar S. M., Prat J. C., Puig M., Wakade A. R. Modification of the evoked release of noradrenaline from the perfused cat spleen by various ions and agents. J Physiol. 1972 Mar;221(3):601–615. doi: 10.1113/jphysiol.1972.sp009770. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kirpekar S. M., Prat J. C., Schiavone M. T. Modification of potassium-evoked release of noradrenaline by various ions and agents. Br J Pharmacol. 1983 Feb;78(2):277–285. doi: 10.1111/j.1476-5381.1983.tb09392.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kirpekar S. M., Wakade A. R., Prat J. C. Effect of tetraethylammonium and barium on the release of noradrenaline from the perfused cat spleen by nerve stimulation and potassium. Naunyn Schmiedebergs Arch Pharmacol. 1976 Jul;294(1):23–29. doi: 10.1007/BF00692781. [DOI] [PubMed] [Google Scholar]
  18. Kirpekar S. M., Wakade A. R. Release of noradrenaline from the cat spleen by potassium. J Physiol. 1968 Feb;194(3):595–608. doi: 10.1113/jphysiol.1968.sp008427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lea T. J., Ashley C. C. Carbon dioxide or bicarbonate ions release Ca2+ from internal stores in crustacean myofibrillar bundles. J Membr Biol. 1981;61(2):115–125. doi: 10.1007/BF02007638. [DOI] [PubMed] [Google Scholar]
  20. Matthews G., Wickelgren W. O. Effects of guanidine on transmitter release and neuronal excitability. J Physiol. 1977 Mar;266(1):69–89. doi: 10.1113/jphysiol.1977.sp011756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Montiel C., Artalejo A. R., García A. G. Effects of the novel dihydropyridine BAY-K-8644 on adrenomedullary catecholamine release evoked by calcium reintroduction. Biochem Biophys Res Commun. 1984 May 16;120(3):851–857. doi: 10.1016/s0006-291x(84)80185-0. [DOI] [PubMed] [Google Scholar]
  22. Niggli V., Sigel E., Carafoli E. The purified Ca2+ pump of human erythrocyte membranes catalyzes an electroneutral Ca2+-H+ exchange in reconstituted liposomal systems. J Biol Chem. 1982 Mar 10;257(5):2350–2356. [PubMed] [Google Scholar]
  23. Orchardson R. The generation of nerve impulses in mammalian axons by changing the concentrations of the normal constituents of extracellular fluid. J Physiol. 1978 Feb;275:177–189. doi: 10.1113/jphysiol.1978.sp012184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schulz I. Bicarbonate transport in the exocrine pancreas. Ann N Y Acad Sci. 1980;341:191–209. doi: 10.1111/j.1749-6632.1980.tb47172.x. [DOI] [PubMed] [Google Scholar]
  25. Steinmetz P. R., Cohen L. H., Husted R. F., Mueller A. Functional organization of proton and bicarbonate transport in turtle urinary bladder. Ann N Y Acad Sci. 1980;341:77–89. doi: 10.1111/j.1749-6632.1980.tb47162.x. [DOI] [PubMed] [Google Scholar]
  26. Thesleff S. Aminopyridines and synaptic transmission. Neuroscience. 1980;5(8):1413–1419. doi: 10.1016/0306-4522(80)90002-0. [DOI] [PubMed] [Google Scholar]
  27. Thoenen H., Haefely W., Staehelin H. Potentiation by tetraethylammonium of the response of the cat spleen to postganglionic sympathetic nerve stimulation. J Pharmacol Exp Ther. 1967 Sep;157(3):532–540. [PubMed] [Google Scholar]
  28. Wakade A. R. A maximum contraction and substantial quantities of tritium can be obtained from tetraethylammonium-treated [3H]-noradrenaline preloaded, rat vas deferens in response to a single electrical shock. Br J Pharmacol. 1980 Mar;68(3):425–436. doi: 10.1111/j.1476-5381.1980.tb14556.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wakade A. R., Wakade T. D. Enhancement of 3H-noradrenaline overflow from cardiac sympathetic nerves by low Ca and tetraethylammonium. Naunyn Schmiedebergs Arch Pharmacol. 1981 Jul;316(4):273–277. doi: 10.1007/BF00501357. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES