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Hyperpolarization-activated chloride currents in parental cells and cells overexpressing ClC-2 are pH-dependent. Current–voltage plots of HACCs generated by parental and S2 cells at four different bath pH values (n = 3–10 for each pH value tested). Error bars indicate the standard error of the mean. Asterisks indicate a significant difference (P < 0.05) in current amplitude at −160 mV from pH 7.45 by anova and Bonferroni ad hoc tests.
