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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Bone. 2007 Mar 21;41(1):68–76. doi: 10.1016/j.bone.2007.03.009

Figure 1. Medium PGE2 and alkaline phosphatase (ALP) activity in cultured calvarial osteoblasts from COX-2 WT and KO mice.

Figure 1

(A) Cumulative PGE2 measured at each medium change. (B) ALP activity calculated at 7 and 17 days of culture in WT cells. (C) ALP staining at 7 days of culture and Von Kossa staining at 21 days of culture. (D) ALP activity in cells cultured for 7 days. Cultures were treated with vehicle (Control), PGE2 (1 μM) or NS398 (0.1 μM). Bars are means ± SEM for n = 3 wells of cells. aSignificant effect of genotype, P<0.01. bSignificant effect of treatment, P<0.01. nd = not detectable.