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. Author manuscript; available in PMC: 2008 Apr 23.
Published in final edited form as: Tetrahedron. 2007 Apr 23;63(17):3601–3607. doi: 10.1016/j.tet.2007.01.074

Figure 9.

Figure 9

Titration of furan-labeled F-TAR2 duplex construct with the Tat12 peptide performed in Tris buffer containing 0.50 μM RNA (see Experimental Section for details). (A) Representative emission spectra for the titration of F-TAR2 duplex with Tat12 peptide. Fluorescence intensity decreased with increasing concentrations of peptide. (B) Fluorescence intensity is plotted against log of peptide concentration. ssRNA, T1 (red) and F-TAR2 duplex (blue). Note the lower emission intensity of the single stranded T1 oligonucleotide. (C) Curve fitting for the titration of duplex F-TAR2 with Tat12 peptide (see Experimental Section for additional details).