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International Journal of Experimental Pathology logoLink to International Journal of Experimental Pathology
. 1995 Feb;76(1):13–19.

Comparison of splenectomy effects as an indication for host response to growth of primary and metastatic tumour cells in two murine tumour systems.

M Michowitz 1, N Donin 1, J Sinai 1, J Leibovici 1
PMCID: PMC1997145  PMID: 7734336

Abstract

The tumour-host relation is of extreme complexity; moreover, it may change during tumour progression. Information regarding this relation may be of importance in appreciating the efficiency of immunotherapy. In the present study, the effect of splenectomy on tumour growth in two murine tumour progression models, the Lewis lung carcinoma and AKR lymphoma, was examined. The effect of spleen ablation on growth of cells derived from primary and metastatic tumour cells was tested. The data obtained showed a differential effect of splenectomy on the growth of primary versus metastatic tumour cells in the two tumour systems, indicating a differential host response induced by the two cell types and/or a differential tumour cell sensitivity to immune reactions. Surprisingly, the spleen appeared to have a defensive role against metastatic tumour cells and a growth enhancing influence on primary tumour cells. The instability of the tumour-host relation may have important implications for the chances of immunotherapy to serve as an efficient cancer treatment.

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Selected References

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  1. Adams D. O. Effector mechanisms of cytolytically activated macrophages. I. Secretion of neutral proteases and effect of protease inhibitors. J Immunol. 1980 Jan;124(1):286–292. [PubMed] [Google Scholar]
  2. Bosslet K., Schirrmacher V. Escape of metastasizing clonal tumor cell variants from tumor-specific cytolytic T lymphocytes. J Exp Med. 1981 Aug 1;154(2):557–562. doi: 10.1084/jem.154.2.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burnet F. M. The concept of immunological surveillance. Prog Exp Tumor Res. 1970;13:1–27. doi: 10.1159/000386035. [DOI] [PubMed] [Google Scholar]
  4. Doherty P. C., Knowles B. B., Wettstein P. J. Immunological surveillance of tumors in the context of major histocompatibility complex restriction of T cell function. Adv Cancer Res. 1984;42:1–65. doi: 10.1016/s0065-230x(08)60455-8. [DOI] [PubMed] [Google Scholar]
  5. Erb P., Baselgia L., Gasser M., Honegger A., Loeffler H. Effect of splenectomy on the growth of adenovirus 12 tumors in hamsters. Experientia. 1974 Aug 15;30(8):943–944. doi: 10.1007/BF01938376. [DOI] [PubMed] [Google Scholar]
  6. Faraci R. P., Marrone J. C., Lesser G. R., Ketcham A. S. The effect of splenectomy on tumor immunity and the metastatic spread of a murine reticulum cell sarcoma. Panminerva Med. 1975 Mar;17(3):59–64. [PubMed] [Google Scholar]
  7. Fisher B., Brown A., Wolmark N., Fisher E. R., Redmond C., Wickerham D. L., Margolese R., Dimitrov N., Pilch Y., Glass A. Evaluation of the worth of corynebacterium parvum in conjunction with chemotherapy as adjuvant treatment for primary breast cancer. Eight-year results from the National Surgical Adjuvant Breast and Bowel Project B-10. Cancer. 1990 Jul 15;66(2):220–227. doi: 10.1002/1097-0142(19900715)66:2<220::aid-cncr2820660205>3.0.co;2-6. [DOI] [PubMed] [Google Scholar]
  8. Gershon R. K., Carter R. L. Factors controlling concomitant immunity in tumor-bearing hamsters: effects of prior splenectomy and tumor removal. J Natl Cancer Inst. 1969 Sep;43(3):533–543. [PubMed] [Google Scholar]
  9. Gorelik E., Fogel M., Feldman M., Segal S. Differences in resistance of metastatic tumor cells and cells from local tumor growth to cytotoxicity of natural killer cells. J Natl Cancer Inst. 1979 Dec;63(6):1397–1404. [PubMed] [Google Scholar]
  10. Grimm E. A., Mazumder A., Zhang H. Z., Rosenberg S. A. Lymphokine-activated killer cell phenomenon. Lysis of natural killer-resistant fresh solid tumor cells by interleukin 2-activated autologous human peripheral blood lymphocytes. J Exp Med. 1982 Jun 1;155(6):1823–1841. doi: 10.1084/jem.155.6.1823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Herberman R. B., Ortaldo J. R. Natural killer cells: their roles in defenses against disease. Science. 1981 Oct 2;214(4516):24–30. doi: 10.1126/science.7025208. [DOI] [PubMed] [Google Scholar]
  12. Ioachim H. L. The stromal reaction of tumors: an expression of immune surveillance. J Natl Cancer Inst. 1976 Sep;57(3):465–475. doi: 10.1093/jnci/57.3.465. [DOI] [PubMed] [Google Scholar]
  13. Israël L., Depierre A., Edelstein R., Cros-Decam J., Maury P. Effect of intranodular B.C.G. in 22 melanoma patients. Panminerva Med. 1975 May-Jun;17(5-6):187–188. [PubMed] [Google Scholar]
  14. Jones J. A., Robinson G., Rees R. C., Baldwin R. W. Immune response of the draining and distal lymph nodes during the progressive grwoth of a chemically-induced transplantable rat hepatoma. Int J Cancer. 1978 Feb 15;21(2):171–178. doi: 10.1002/ijc.2910210208. [DOI] [PubMed] [Google Scholar]
  15. Kerbel R. S. Implications of immunological heterogeneity of tumours. Nature. 1979 Aug 2;280(5721):358–360. doi: 10.1038/280358a0. [DOI] [PubMed] [Google Scholar]
  16. Klefström P., Gröhn P., Heinonen E., Holsti L., Holsti P. Adjuvant postoperative radiotherapy, chemotherapy, and immunotherapy in stage III breast cancer. II. 5-year results and influence of levamisole. Cancer. 1987 Sep 1;60(5):936–942. doi: 10.1002/1097-0142(19870901)60:5<936::aid-cncr2820600504>3.0.co;2-p. [DOI] [PubMed] [Google Scholar]
  17. Leibovici J., Michowitz M., Argaman H. Change in the role of the spleen from protective to harmful following tumor progression in AKR lymphoma. Invasion Metastasis. 1990;10(5):289–300. [PubMed] [Google Scholar]
  18. Mathé G., Pouillart P., Lapeyraque F. Active immunotherapy of L1210 leukaemia applied after the graft of tumour cells. Br J Cancer. 1969 Dec;23(4):814–824. doi: 10.1038/bjc.1969.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Michowitz M., Kopel S., Hoenig S., Leibovici J. Effect of splenectomy on treatment of Lewis lung carcinoma by an immunomodulatory polysaccharide and a cytotoxic agent. J Surg Oncol. 1987 Apr;34(4):268–271. doi: 10.1002/jso.2930340412. [DOI] [PubMed] [Google Scholar]
  20. Milas L., Mujagic H. The effect of splenectomy on fibrosarcoma "metastases" in lungs of mice. Int J Cancer. 1973 Jan 15;11(1):186–190. doi: 10.1002/ijc.2910110122. [DOI] [PubMed] [Google Scholar]
  21. Mosley J. G., Sadler T. E., Castro J. E. Effects of amputation and Corynebacterium parvum on tumour metastases in mice. Br J Cancer. 1978 Apr;37(4):571–575. doi: 10.1038/bjc.1978.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Nelson D. S., Kearney R. Macrophages and lymphoid tissues in mice with concomitant tumour immunity. Br J Cancer. 1976 Sep;34(3):221–226. doi: 10.1038/bjc.1976.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Old L. J., Boyse E. A. Antigens of tumors and leukemias induced by viruses. Fed Proc. 1965 Sep-Oct;24(5):1009–1017. [PubMed] [Google Scholar]
  24. PREHN R. T., MAIN J. M. Immunity to methylcholanthrene-induced sarcomas. J Natl Cancer Inst. 1957 Jun;18(6):769–778. [PubMed] [Google Scholar]
  25. Prehn R. T., Prehn L. M. The flip side of tumor immunity. Arch Surg. 1989 Jan;124(1):102–106. doi: 10.1001/archsurg.1989.01410010112022. [DOI] [PubMed] [Google Scholar]
  26. Prehn R. T. Tumor progression and homeostasis. Adv Cancer Res. 1976;23:203–236. doi: 10.1016/s0065-230x(08)60547-3. [DOI] [PubMed] [Google Scholar]
  27. Rojas A. F., Feierstein J. N., Mickiewicz E., Glait H., Olivari A. J. Levamisole in advanced human breast cancer. Lancet. 1976 Jan 31;1(7953):211–215. doi: 10.1016/s0140-6736(76)91337-4. [DOI] [PubMed] [Google Scholar]
  28. Sato N., Michaelides M. C., Wallack M. K. Characterization of tumorigenicity, mortality, metastasis, and splenomegaly of two cultured murine colon lines. Cancer Res. 1981 Jun;41(6):2267–2272. [PubMed] [Google Scholar]
  29. Sato N., Michaelides M. C., Wallack M. K. Effect of splenectomy on the growth of murine colon tumors. J Surg Oncol. 1983 Feb;22(2):73–76. doi: 10.1002/jso.2930220202. [DOI] [PubMed] [Google Scholar]
  30. Treves A. J., Cohen I. R., Feldman M. A syngeneic metastatic tumor model in mice: the natural immune response of the host and its manipulation. Isr J Med Sci. 1976 Apr-May;12(4-5):369–384. [PubMed] [Google Scholar]
  31. Vose B. M., Moore M. Human tumor-infiltrating lymphocytes: a marker of host response. Semin Hematol. 1985 Jan;22(1):27–40. [PubMed] [Google Scholar]
  32. Witting C., Hultsch E. Effect of splenectomy on B- and T-lymphocytes and a benzpyrene-induced murine sarcoma. Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1978 Oct 30;92(3):267–274. doi: 10.1007/BF00461649. [DOI] [PubMed] [Google Scholar]
  33. Yamashina K., Fulton A., Heppner G. Differential sensitivity of metastatic versus nonmetastatic mammary tumor cells to macrophage-mediated cytostasis. J Natl Cancer Inst. 1985 Oct;75(4):765–770. [PubMed] [Google Scholar]

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