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International Journal of Experimental Pathology logoLink to International Journal of Experimental Pathology
. 1995 Apr;76(2):149–155.

Mycobacterial granulomatous inflammation in the ulcerated caecum of indomethacin-treated rats.

A Anthony 1, A P Dhillon 1, H Fidler 1, J McFadden 1, O Billington 1, G Nygard 1, R E Pounder 1, A J Wakefield 1
PMCID: PMC1997147  PMID: 7786765

Abstract

We report mycobacterial granulomatous inflammation in the ulcerated caecum of rats that received indomethacin. Two groups of male rats were treated with dietary indomethacin 3 mg/kg/day or untreated diet for 3 weeks. Six out of 8 indomethacin treated rats showed both ulceration and inflammation of the caecal mucosa. Two of the rats showing caecal ulceration also showed distinct granulomatous inflammation of the caecal mucosa and acid-fast bacilli were identified within granulomata. None of the other indomethacin treated or control rats contained acid-fast bacilli within caecal tissue sections but they were present, in the same sections, within the lumen of most rats in both groups. Polymerase chain reaction analysis identified the mycobacterial 65 kDa GroEL gene within control and granulomatous caecal tissue. In a repeat of indomethacin administration to a third group of rats, culture of both non-granulomatous caecal tissue (containing histologically identified luminal acid-fast bacilli) and faecal samples for mycobacteria was negative.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bentley G., Webster J. H. Gastro-intestinal tuberculosis. A 10-year review. Br J Surg. 1967 Feb;54(2):90–96. doi: 10.1002/bjs.1800540203. [DOI] [PubMed] [Google Scholar]
  2. Davies M. H., Elias E., Acharya S., Cotton W., Faulder G. C., Fryer A. A., Strange R. C. GSTM1 null polymorphism at the glutathione S-transferase M1 locus: phenotype and genotype studies in patients with primary biliary cirrhosis. Gut. 1993 Apr;34(4):549–553. doi: 10.1136/gut.34.4.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fidler H. M., Rook G. A., Johnson N. M., McFadden J. Mycobacterium tuberculosis DNA in tissue affected by sarcoidosis. BMJ. 1993 Feb 27;306(6877):546–549. doi: 10.1136/bmj.306.6877.546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hermon-Taylor J. Causation of Crohn's disease: the impact of clusters. Gastroenterology. 1993 Feb;104(2):643–646. doi: 10.1016/0016-5085(93)90438-I. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Robinson H. J., Phares H. F., Graessle O. E. Effects of indomethacin on acute, subacute, and latent infections in mice and rats. J Bacteriol. 1968 Jul;96(1):6–13. doi: 10.1128/jb.96.1.6-13.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Saboor S. A., Johnson N. M., McFadden J. Detection of mycobacterial DNA in sarcoidosis and tuberculosis with polymerase chain reaction. Lancet. 1992 Apr 25;339(8800):1012–1015. doi: 10.1016/0140-6736(92)90535-b. [DOI] [PubMed] [Google Scholar]
  7. Sanderson J. D., Hermon-Taylor J. Mycobacterial diseases of the gut: some impact from molecular biology. Gut. 1992 Feb;33(2):145–147. doi: 10.1136/gut.33.2.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sanderson J. D., Moss M. T., Tizard M. L., Hermon-Taylor J. Mycobacterium paratuberculosis DNA in Crohn's disease tissue. Gut. 1992 Jul;33(7):890–896. doi: 10.1136/gut.33.7.890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Shah S., Thomas V., Mathan M., Chacko A., Chandy G., Ramakrishna B. S., Rolston D. D. Colonoscopic study of 50 patients with colonic tuberculosis. Gut. 1992 Mar;33(3):347–351. doi: 10.1136/gut.33.3.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stainsby K. J., Lowes J. R., Allan R. N., Ibbotson J. P. Antibodies to Mycobacterium paratuberculosis and nine species of environmental mycobacteria in Crohn's disease and control subjects. Gut. 1993 Mar;34(3):371–374. doi: 10.1136/gut.34.3.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tomasson H. O., Brennan M., Bass M. J. Tuberculosis and nonsteroidal anti-inflammatory drugs. Can Med Assoc J. 1984 Feb 1;130(3):275–278. [PMC free article] [PubMed] [Google Scholar]
  12. Vafiadis I., Boletis J., Papastathi H., Delladetsima J., Stathakis C., Hatzakis A., Kostakis A., Vosnides G. Hepatitis C virus infection among Greek renal transplant patients. Gut. 1993;34(2 Suppl):S57–S58. doi: 10.1136/gut.34.2_suppl.s57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Van Kruiningen H. J., Chiodini R. J., Thayer W. R., Coutu J. A., Merkal R. S., Runnels P. L. Experimental disease in infant goats induced by a Mycobacterium isolated from a patient with Crohn's disease. A preliminary report. Dig Dis Sci. 1986 Dec;31(12):1351–1360. doi: 10.1007/BF01299814. [DOI] [PubMed] [Google Scholar]
  14. Van Kruiningen H. J., Colombel J. F., Cartun R. W., Whitlock R. H., Koopmans M., Kangro H. O., Hoogkamp-Korstanje J. A., Lecomte-Houcke M., Devred M., Paris J. C. An in-depth study of Crohn's disease in two French families. Gastroenterology. 1993 Feb;104(2):351–360. doi: 10.1016/0016-5085(93)90401-W. [DOI] [PMC free article] [PubMed] [Google Scholar]

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