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International Journal of Experimental Pathology logoLink to International Journal of Experimental Pathology
. 1995 Oct;76(5):317–330.

The cochlear lesion in experimental bacterial meningitis of the rabbit.

M P Osborne 1, S D Comis 1, M J Tarlow 1, J Stephen 1
PMCID: PMC1997190  PMID: 7488546

Abstract

Sensorineural hearing loss was studied in a rabbit model of experimental bacterial meningitis using electrophysiological and ultrastructural techniques. Hearing impairment was monitored by auditory brain-stem evoked responses (ABERs) and concomitant structural lesions were identified by both transmission (TEM) and scanning (SEM) electron microscopy. Meningitis was induced by intra-cerebrospinal fluid injection of either Escherichia coli (strain 2073 and type K-12) or Haemophilus influenzae type b. Auditory loss of approximately equal to 10 dB occurred in all rabbits by about 10 hours post infection and progressed in severity until by 20 h following infection, hearing losses up to and > 60 dB were obtained. At levels of hearing loss < 20 dB ultrastructural damage to the organ of Corti was barely detectable. With greater levels of hearing loss, patchy structural damage to hair cells, synaptic nerve terminals, supporting cells and inner spiral sulcus cells and cells of the stria vascularis was clearly evident. Bacteria were found in scala tympani, the basilar membrane, the organ of Corti, scala media, the spiral ligament and at the margin of the stria vascularis. Evidence of bleeding was found in some cochleas; erythrocytes were found in scala tympani, scala media, amongst hair cells and beneath the tectorial membrane. The results show that hearing loss is associated with bacterial invasion and damage to the organ of Corti and that the cause of hearing loss is likely to result from multiple lesions within the cochlea. Lesions to sensory cells almost certainly will produce permanent hearing loss. Lesions to supporting cells, nerve terminals and to stria vascularis may well produce only temporary hearing loss.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhatt S. M., Cabellos C., Nadol J. B., Jr, Halpin C., Lauretano A., Xu W. Z., Tuomanen E. The impact of dexamethasone on hearing loss in experimental pneumococcal meningitis. Pediatr Infect Dis J. 1995 Feb;14(2):93–96. doi: 10.1097/00006454-199502000-00002. [DOI] [PubMed] [Google Scholar]
  2. Bhatt S. M., Lauretano A., Cabellos C., Halpin C., Levine R. A., Xu W. Z., Nadol J. B., Jr, Tuomanen E. Progression of hearing loss in experimental pneumococcal meningitis: correlation with cerebrospinal fluid cytochemistry. J Infect Dis. 1993 Mar;167(3):675–683. doi: 10.1093/infdis/167.3.675. [DOI] [PubMed] [Google Scholar]
  3. Bhatt S., Halpin C., Hsu W., Thedinger B. A., Levine R. A., Tuomanen E., Nadol J. B., Jr Hearing loss and pneumococcal meningitis: an animal model. Laryngoscope. 1991 Dec;101(12 Pt 1):1285–1292. doi: 10.1002/lary.5541011206. [DOI] [PubMed] [Google Scholar]
  4. Blank A. L., Davis G. L., VanDeWater T. R., Ruben R. J. Acute Streptococcus pneumoniae meningogenic labyrinthitis. An experimental guinea pig model and literature review. Arch Otolaryngol Head Neck Surg. 1994 Dec;120(12):1342–1346. doi: 10.1001/archotol.1994.01880360040008. [DOI] [PubMed] [Google Scholar]
  5. Brookhouser P. E., Auslander M. C., Meskan M. E. The pattern and stability of postmeningitic hearing loss in children. Laryngoscope. 1988 Sep;98(9):940–948. doi: 10.1288/00005537-198809000-00007. [DOI] [PubMed] [Google Scholar]
  6. Comis S. D., Osborne M. P., Stephen J., Tarlow M. J., Hayward T. L., Mitchell T. J., Andrew P. W., Boulnois G. J. Cytotoxic effects on hair cells of guinea pig cochlea produced by pneumolysin, the thiol activated toxin of Streptococcus pneumoniae. Acta Otolaryngol. 1993 Mar;113(2):152–159. doi: 10.3109/00016489309135784. [DOI] [PubMed] [Google Scholar]
  7. Comis S. D., Osborne M. P., Tarlow M. J. The effect of bacterial endotoxin upon the morphology of the tectorial membrane and stereocilia in the guinea pig cochlea. Scanning Microsc. 1991 Dec;5(4):1129–1134. [PubMed] [Google Scholar]
  8. Corwin J. T., Warchol M. E. Auditory hair cells: structure, function, development, and regeneration. Annu Rev Neurosci. 1991;14:301–333. doi: 10.1146/annurev.ne.14.030191.001505. [DOI] [PubMed] [Google Scholar]
  9. Dodge P. R., Davis H., Feigin R. D., Holmes S. J., Kaplan S. L., Jubelirer D. P., Stechenberg B. W., Hirsh S. K. Prospective evaluation of hearing impairment as a sequela of acute bacterial meningitis. N Engl J Med. 1984 Oct 4;311(14):869–874. doi: 10.1056/NEJM198410043111401. [DOI] [PubMed] [Google Scholar]
  10. Fortnum H. M. Hearing impairment after bacterial meningitis: a review. Arch Dis Child. 1992 Sep;67(9):1128–1133. doi: 10.1136/adc.67.9.1128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Geiman B. J., Smith A. L. Dexamethasone and bacterial meningitis. A meta-analysis of randomized controlled trials. West J Med. 1992 Jul;157(1):27–31. [PMC free article] [PubMed] [Google Scholar]
  12. Guiscafré H., Benitez-Díaz L., Martínez M. C., Muñoz O. Reversible hearing loss after meningitis. Prospective assessment using auditory evoked responses. Ann Otol Rhinol Laryngol. 1984 May-Jun;93(3 Pt 1):229–232. doi: 10.1177/000348948409300308. [DOI] [PubMed] [Google Scholar]
  13. Henneford G. E., Lindsay J. R. Deaf-mutism due to meningogenic labyrinthitis. Laryngoscope. 1968 Feb;78(2):251–261. doi: 10.1288/00005537-196802000-00006. [DOI] [PubMed] [Google Scholar]
  14. Huang M., Dulon D., Schacht J. Outer hair cells as potential targets of inflammatory mediators. Ann Otol Rhinol Laryngol Suppl. 1990 Jun;148:35–38. doi: 10.1177/00034894900990s610. [DOI] [PubMed] [Google Scholar]
  15. Jewett D. L., Williston J. S. Auditory-evoked far fields averaged from the scalp of humans. Brain. 1971;94(4):681–696. doi: 10.1093/brain/94.4.681. [DOI] [PubMed] [Google Scholar]
  16. Kaplan S. L., Hawkins E. P., Kline M. W., Patrick G. S., Mason E. O., Jr Invasion of the inner ear by Haemophilus influenzae type b in experimental meningitis. J Infect Dis. 1989 May;159(5):923–930. doi: 10.1093/infdis/159.5.923. [DOI] [PubMed] [Google Scholar]
  17. Kay R. The site of the lesion causing hearing loss in bacterial meningitis: a study of experimental streptococcal meningitis in guinea-pigs. Neuropathol Appl Neurobiol. 1991 Dec;17(6):485–493. doi: 10.1111/j.1365-2990.1991.tb00751.x. [DOI] [PubMed] [Google Scholar]
  18. Levy S. B., Sullivan N., Gorbach S. L. Pathogenicity of conventional and debilitated Escherichia coli K12. Nature. 1978 Jul 27;274(5669):395–396. doi: 10.1038/274395a0. [DOI] [PubMed] [Google Scholar]
  19. Morrison D. C., Ryan J. L. Endotoxins and disease mechanisms. Annu Rev Med. 1987;38:417–432. doi: 10.1146/annurev.me.38.020187.002221. [DOI] [PubMed] [Google Scholar]
  20. Mustafa M. M., Ramilo O., Mertsola J., Risser R. C., Beutler B., Hansen E. J., McCracken G. H., Jr Modulation of inflammation and cachectin activity in relation to treatment of experimental Hemophilus influenzae type b meningitis. J Infect Dis. 1989 Nov;160(5):818–825. doi: 10.1093/infdis/160.5.818. [DOI] [PubMed] [Google Scholar]
  21. Naess A., Halstensen A., Nyland H., Pedersen S. H., Møller P., Borgmann R., Larsen J. L., Haga E. Sequelae one year after meningococcal disease. Acta Neurol Scand. 1994 Feb;89(2):139–142. doi: 10.1111/j.1600-0404.1994.tb01649.x. [DOI] [PubMed] [Google Scholar]
  22. Osborne M. P., Comis S. D. High resolution scanning electron microscopy of stereocilia in the cochlea of normal, postmortem, and drug-treated guinea pigs. J Electron Microsc Tech. 1990 Jul;15(3):245–260. doi: 10.1002/jemt.1060150305. [DOI] [PubMed] [Google Scholar]
  23. Osborne M. P., Comis S. D. Preparation of inner ear sensory hair bundles for high resolution scanning electron microscopy. Scanning Microsc. 1991 Jun;5(2):555–564. [PubMed] [Google Scholar]
  24. Rodriguez A. F., Kaplan S. L., Hawkins E. P., Mason E. O., Jr Hematogenous pneumococcal meningitis in the infant rat: description of a model. J Infect Dis. 1991 Dec;164(6):1207–1209. doi: 10.1093/infdis/164.6.1207. [DOI] [PubMed] [Google Scholar]
  25. Roos K. L. Dexamethasone and nonsteroidal anti-inflammatory agents in the treatment of bacterial meningitis. Clin Ther. 1990 Jul-Aug;12(4):290–289. [PubMed] [Google Scholar]
  26. Rosenhall U., Kankkunen A. Hearing alterations following meningitis. 2. Variable hearing. Ear Hear. 1981 Jul-Aug;2(4):170–176. doi: 10.1097/00003446-198107000-00006. [DOI] [PubMed] [Google Scholar]
  27. Sulc P., Hengstler B., Krinke G., O'Reilly T., Zak O. Experimental rabbit model of meningitis produced by Haemophilus influenzae serotype c. J Med Microbiol. 1992 May;36(5):312–317. doi: 10.1099/00222615-36-5-312. [DOI] [PubMed] [Google Scholar]
  28. Syrogiannopoulos G. A., Hansen E. J., Erwin A. L., Munford R. S., Rutledge J., Reisch J. S., McCracken G. H., Jr Haemophilus influenzae type b lipooligosaccharide induces meningeal inflammation. J Infect Dis. 1988 Feb;157(2):237–244. doi: 10.1093/infdis/157.2.237. [DOI] [PubMed] [Google Scholar]
  29. Tarlow M. J., Comis S. D., Osborne M. P. Endotoxin induced damage to the cochlea in guinea pigs. Arch Dis Child. 1991 Feb;66(2):181–184. doi: 10.1136/adc.66.2.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Tuomanen E., Tomasz A., Hengstler B., Zak O. The relative role of bacterial cell wall and capsule in the induction of inflammation in pneumococcal meningitis. J Infect Dis. 1985 Mar;151(3):535–540. doi: 10.1093/infdis/151.3.535. [DOI] [PubMed] [Google Scholar]
  31. Vienny H., Despland P. A., Lütschg J., Deonna T., Dutoit-Marco M. L., Gander C. Early diagnosis and evolution of deafness in childhood bacterial meningitis: a study using brainstem auditory evoked potentials. Pediatrics. 1984 May;73(5):579–586. [PubMed] [Google Scholar]
  32. Wald E. R., Kaplan S. L., Mason E. O., Jr, Sabo D., Ross L., Arditi M., Wiedermann B. L., Barson W., Kim K. S., Yogov R. Dexamethasone therapy for children with bacterial meningitis. Meningitis Study Group. Pediatrics. 1995 Jan;95(1):21–28. [PubMed] [Google Scholar]

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