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Journal of Experimental Pathology (Oxford, England) logoLink to Journal of Experimental Pathology (Oxford, England)
. 1990 Feb;71(1):63–68.

Biliary lipid secretion during the prereplicative phase of rat liver regeneration.

F Perez-Barriocanal 1, J C Perez-Antona 1, P Regueiro 1, G R Villanueva 1, J J Marin 1
PMCID: PMC1998681  PMID: 2310616

Abstract

The prereplicative phase-related changes in spontaneous and taurocholate-induced biliary lipid secretion were studied in anaesthetized male Wistar rats (250 g). Rats underwent two-thirds hepatectomy 1, 6 or 12 h before starting to collect bile samples. As compared with non-hepatectomized rats, biliary lipid secretion was increased at 1 h after hepatectomy and then restored to values similar to the control group up to 12 h after hepatectomy. In separate experiments, taurocholate was infused (200 nmol/min/g calculated liver weight) through the jugular vein over 80 min. Both taurocholate-induced bile flow and bile acid output were similar in control and hepatectomized rats, regardless of the time of the prereplicative phase considered. By contrast, taurocholate-induced lecithin and cholesterol outputs were markedly modified. The former was lowered throughout the prereplicative phase, whereas the latter increased at 6 h and decreased at 12 h. In summary, these results indicate that shortly after hepatectomy bile acid-induced biliary lipid secretion is profoundly modified, probably due to changes in the plasma membrane involved in preparing the hepatocyte to enter the cell cycle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUCHER N. L. REGENERATION OF MAMMALIAN LIVER. Int Rev Cytol. 1963;15:245–300. doi: 10.1016/s0074-7696(08)61119-5. [DOI] [PubMed] [Google Scholar]
  2. Bolton C. H., Nicholls J. S., Heaton K. W. Estimation of cholesterol in bile: assessment of an enzymatic method. Clin Chim Acta. 1980 Aug 4;105(2):225–230. doi: 10.1016/0009-8981(80)90464-7. [DOI] [PubMed] [Google Scholar]
  3. Bruscalupi G., Curatola G., Lenaz G., Leoni S., Mangiantini M. T., Mazzanti L., Spagnuolo S., Trentalance A. Plasma membrane changes associated with rat liver regeneration. Biochim Biophys Acta. 1980 Apr 10;597(2):263–273. doi: 10.1016/0005-2736(80)90104-2. [DOI] [PubMed] [Google Scholar]
  4. Bucher N. L. Experimental aspects of hepatic regeneration. N Engl J Med. 1967 Sep 28;277(13):686–contd. doi: 10.1056/NEJM196709282771306. [DOI] [PubMed] [Google Scholar]
  5. Enrich C., Bachs O., Soriano M., Serratosa J. Changes in sinusoidal plasma membrane enzyme activities during the pre-replicative phase of liver regeneration. Biochim Biophys Acta. 1986 Oct 9;861(2):381–384. doi: 10.1016/0005-2736(86)90443-8. [DOI] [PubMed] [Google Scholar]
  6. Field F. J., Mathur S. N., LaBrecque D. R. Cholesterol metabolism in regenerating liver of the rat. Am J Physiol. 1985 Dec;249(6 Pt 1):G679–G684. doi: 10.1152/ajpgi.1985.249.6.G679. [DOI] [PubMed] [Google Scholar]
  7. Fukano S., Saitoh Y., Uchida K., Akiyoshi T., Takeda K. Bile acid metabolism in partially hepatectomized rats. Steroids. 1985 Mar-Apr;45(3-4):209–227. doi: 10.1016/0039-128x(85)90071-6. [DOI] [PubMed] [Google Scholar]
  8. Gärtner U., Stockert R. J., Morell A. G., Wolkoff A. W. Modulation of the transport of bilirubin and asialoorosomucoid during liver regeneration. Hepatology. 1981 Mar-Apr;1(2):99–106. doi: 10.1002/hep.1840010203. [DOI] [PubMed] [Google Scholar]
  9. JAMES A. T. Qualitative and quantitative determination of the fatty acids by gas-liquid chromatography. Methods Biochem Anal. 1960;8:1–59. doi: 10.1002/9780470110249.ch1. [DOI] [PubMed] [Google Scholar]
  10. Sanz A., Zamora S., López M. A., Esteller A. Relative changes in bile secretion during rat liver regeneration. Rev Esp Fisiol. 1983 Dec;39(4):455–459. [PubMed] [Google Scholar]
  11. Takayama M., Itoh S., Nagasaki T., Tanimizu I. A new enzymatic method for determination of serum choline-containing phospholipids. Clin Chim Acta. 1977 Aug 15;79(1):93–98. doi: 10.1016/0009-8981(77)90465-x. [DOI] [PubMed] [Google Scholar]
  12. Uesugi T., Bognacki J., Levine W. G. Biliary excretion of drugs in the rat during liver regeneration. Biochem Pharmacol. 1976 May 15;25(10):1187–1193. doi: 10.1016/0006-2952(76)90367-1. [DOI] [PubMed] [Google Scholar]
  13. WEINBREN K., BILLING B. H. Hepatic clearance of bilirubin as an index of cellular function in the regenerating rat liver. Br J Exp Pathol. 1956 Apr;37(2):199–204. [PMC free article] [PubMed] [Google Scholar]

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