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Journal of Experimental Pathology (Oxford, England) logoLink to Journal of Experimental Pathology (Oxford, England)
. 1990 Jun;71(3):337–358.

Histopathology of N-methyl-N-nitrosourea-induced mesenchymal tumours of the rat urinary bladder.

E Kunze 1, F Ruschitzka 1, K Schwalbe 1
PMCID: PMC1998692  PMID: 2164825

Abstract

The present study reports the induction, histopathology, immunocytochemistry, growth pattern and proliferative behaviour of mesenchymal tumours of the urinary bladder of rats induced by a single intravesical dose (5 mg/kg/body weight) of N-methyl-N-nitrosourea (MNU). In 14 of 283 female Wistar rats (incidence: 4.9%). 16 non-epithelial tumours had developed after an experimental period of 14 months. The most common histological type induced was of fibrohistiocytic origin (eight benign-appearing and three malignant fibrous histiocytomas). Furthermore, two pure histiocytomas (one benign and one malignant) and three capillary and cavernous haemangiomas were produced. Since no metastases occurred and no clear-cut distinction between a merely expansive and a truly invasive growth was possible, the diagnosis of malignancy was based on the degree of cellular atypia and the mitotic activity. The benign-appearing fibrous histiocytomas showed a mean mitotic index of 0.06% and the malignant fibrous histiocytomas of 0.34%. The mitotic activity of the malignant histiocytoma was threefold (0.78%) as high as in the benign-appearing histiocytoma (0.25%). There exist close morphological similarities between the induced mesenchymal tumours in the rat and those occurring in the human bladder. Although the spectrum of histological types of mesenchymal tumours seen in the rat bladder was narrower than that encountered in man, MNU seems suitable for further studying the histogenesis, histopathology and biology of experimentally induced non-epithelial bladder neoplasms to gain information for a better understanding of the pathogenesis of human disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Althoff J., Lijinsky W. Urinary bladder neoplasms in Syrian hamsters after administration of N-nitroso-N-methyl-N-dodecylamine. Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1977 Dec 15;90(3):227–231. doi: 10.1007/BF00284296. [DOI] [PubMed] [Google Scholar]
  2. Anderson J. D., Scardino P., Smith R. B. Inflammatory fibrous histiocytoma presenting as a renal pelvic and bladder mass. J Urol. 1977 Sep;118(3):470–471. doi: 10.1016/s0022-5347(17)58065-9. [DOI] [PubMed] [Google Scholar]
  3. Brooks P. T., Scally J. K. Bladder neurofibromas causing ureteric obstruction in Von Recklinghausen's disease. Clin Radiol. 1985 Sep;36(5):537–538. doi: 10.1016/s0009-9260(85)80215-4. [DOI] [PubMed] [Google Scholar]
  4. CHOPRA R. P., KHANNA S. D., SAIGAL R. K. HEMANGIOMA OF THE BLADDER: A CASE REPORT. J Urol. 1965 Jul;94:56–57. doi: 10.1016/S0022-5347(17)63568-7. [DOI] [PubMed] [Google Scholar]
  5. Chitiyo M. E. Primary osteogenic sarcoma of the urinary bladder. J Pathol. 1973 Sep;111(1):53–56. doi: 10.1002/path.1711110109. [DOI] [PubMed] [Google Scholar]
  6. DEAN A. L., ASH J. E. Study of the bladder tumors in the registry of the American Urological Association. J Urol. 1950 Apr;63(4):618–624. doi: 10.1016/S0022-5347(17)68806-2. [DOI] [PubMed] [Google Scholar]
  7. Deerberg F., Rehm S., Jostmeyer H. H. Spontaneous urinary bladder tumors in DA/Han rats: a feasible model of human bladder cancer. J Natl Cancer Inst. 1985 Dec;75(6):1113–1121. [PubMed] [Google Scholar]
  8. Frith C. H. Morphologic classification of inflammatory, nonspecific, and proliferative lesions of the urinary bladder of mice. Invest Urol. 1979 May;16(6):435–444. [PubMed] [Google Scholar]
  9. Fuleihan F. M., Cordonnier J. J. Hemangioma of the bladder: report of a case and review of the literature. J Urol. 1969 Nov;102(5):581–585. doi: 10.1016/s0022-5347(17)62204-3. [DOI] [PubMed] [Google Scholar]
  10. Goodman A. J., Greaney M. G. Malignant fibrous histiocytoma of the bladder. Br J Urol. 1985 Feb;57(1):106–107. doi: 10.1111/j.1464-410x.1985.tb08996.x. [DOI] [PubMed] [Google Scholar]
  11. Gottesman J. E., Seale R. H. Cavernous haemangioma of the bladder. Br J Urol. 1983 Aug;55(4):450–451. [PubMed] [Google Scholar]
  12. Hard G. C., Butler W. H. Ultrastructural analysis of renal mesenchymal tumor induced in the rat by dimethylnitrosamine. Cancer Res. 1971 Mar;31(3):348–365. [PubMed] [Google Scholar]
  13. Harrison G. S. Malignant fibrous histiocytoma of the bladder. Br J Urol. 1986 Aug;58(4):457–458. doi: 10.1111/j.1464-410x.1986.tb09107.x. [DOI] [PubMed] [Google Scholar]
  14. Hendry W. F., Vinnicombe J. Haemangioma of bladder in children and young adults. Br J Urol. 1971 Jun;43(3):309–316. doi: 10.1111/j.1464-410x.1971.tb12046.x. [DOI] [PubMed] [Google Scholar]
  15. Henriksen O. B., Mogensen P., Engelholm A. J. Inflammatory fibrous histiocytoma of the urinary bladder: clinicopathological report of a case. Acta Pathol Microbiol Immunol Scand A. 1982 Sep;90(5):333–337. doi: 10.1111/j.1699-0463.1982.tb00103_90a.x. [DOI] [PubMed] [Google Scholar]
  16. Hicks R. M., Chowaniec J. Experimental induction, histology, and ultrastructure of hyperplasia and neoplasia of the urinary bladder epithelium. Int Rev Exp Pathol. 1978;18:199–280. [PubMed] [Google Scholar]
  17. Karol J. B., Eason A. A., Tanagho E. A. Fibrous histiocytoma of bladder. Urology. 1977 Dec;10(6):593–595. doi: 10.1016/0090-4295(77)90113-3. [DOI] [PubMed] [Google Scholar]
  18. Kroes R., Garbis-Berkvens J. M., de Vries T., van Nesselrooy H. J. Histopathological profile of a Wistar rat stock including a survey of the literature. J Gerontol. 1981 May;36(3):259–279. doi: 10.1093/geronj/36.3.259. [DOI] [PubMed] [Google Scholar]
  19. Kunze E., Albrecht H., Wöltjen H. H., Schauer A. Die reparative Regeneration des Rattenurothels nach partieller Cystektomie und ihre Bedeutung für die Carcinogenese. J Cancer Res Clin Oncol. 1979 Oct;95(2):159–175. doi: 10.1007/BF00401010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kunze E. Development of urinary bladder cancer in the rat. Curr Top Pathol. 1979;67:145–232. doi: 10.1007/978-3-642-67292-7_3. [DOI] [PubMed] [Google Scholar]
  21. Kunze E., Graewe T., Scherber S., Weber J., Gellhar P. Cell cycle dependence of N-methyl-N-nitrosourea-induced tumour development in the proliferating, partially resected rat urinary bladder. Br J Exp Pathol. 1989 Apr;70(2):125–142. [PMC free article] [PubMed] [Google Scholar]
  22. Kunze E., Weber J., Gellhar P., Graewe T., Scherber S. Synchronization of stimulated urothelial proliferation. Experimental models for cell cycle specific testing of bladder carcinogens. J Cancer Res Clin Oncol. 1987;113(1):8–14. doi: 10.1007/BF00389960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mills S. E., Bova G. S., Wick M. R., Young R. H. Leiomyosarcoma of the urinary bladder. A clinicopathologic and immunohistochemical study of 15 cases. Am J Surg Pathol. 1989 Jun;13(6):480–489. doi: 10.1097/00000478-198906000-00004. [DOI] [PubMed] [Google Scholar]
  24. Numoto S., Yoshida M., Otsuka H. Bladder carcinogenesis in mice induced by N-butyl-N-(4-hydroxybutyl) nitrosamine and N-ethyl-N-(4-hydroxybutyl)-nitrosamine with reference to the effect of cyclophosphamide. Gan. 1981 Oct;72(5):647–654. [PubMed] [Google Scholar]
  25. Pamukcu A. M., Price J. M., Bryan G. T. Naturally occurring and braken-fern-induced bovine urinary bladder tumors. Clinical and morphological characteristics. Vet Pathol. 1976;13(2):110–122. doi: 10.1177/030098587601300205. [DOI] [PubMed] [Google Scholar]
  26. RICHES E. W. A case of cavernous haemangioma of the bladder. Br J Urol. 1951 Sep;23(3):204–208. doi: 10.1111/j.1464-410x.1951.tb03613.x. [DOI] [PubMed] [Google Scholar]
  27. Rosi P., Selli C., Carini M., Rosi M. F. Myxoid liposarcoma of the bladder. J Urol. 1983 Sep;130(3):560–561. doi: 10.1016/s0022-5347(17)51304-x. [DOI] [PubMed] [Google Scholar]
  28. SILBAR J. D., SILBAR S. J. Leiomyosarcoma of bladder: three case reports and a review of literature. J Urol. 1955 Jan;73(1):103–111. doi: 10.1016/S0022-5347(17)67372-5. [DOI] [PubMed] [Google Scholar]
  29. Seo I. S., Clark S. A., McGovern F. D., Clark D. L., Johnson E. H. Leimyosarcoma of the urinary bladder. 13 years after cyclophosphamide therapy for Hodgkin's disease. Cancer. 1985 Apr 1;55(7):1597–1603. doi: 10.1002/1097-0142(19850401)55:7<1597::aid-cncr2820550731>3.0.co;2-0. [DOI] [PubMed] [Google Scholar]
  30. Squire R. A., Brinkhous K. M., Peiper S. C., Firminger H. I., Mann R. B., Strandberg J. D. Histiocytic sarcoma with a granuloma-like component occurring in a large colony of Sprague-Dawley rats. Am J Pathol. 1981 Oct;105(1):21–30. [PMC free article] [PubMed] [Google Scholar]
  31. Stanley K. E., Jr Hemangioma-lymphangioma of the bladder in a child: report of a case with associated hemangiomas of the external genitalia. J Urol. 1966 Jul;96(1):51–54. doi: 10.1016/S0022-5347(17)63203-8. [DOI] [PubMed] [Google Scholar]
  32. Stearns M. M., Mitchell A. D., Powell N. E., Wood W. G., Mebust W. K. Fibrous histiocytoma of the bladder. J Urol. 1976 Jan;115(1):114–115. doi: 10.1016/s0022-5347(17)59090-4. [DOI] [PubMed] [Google Scholar]
  33. Stroup R. M., Chang Y. C. Angiosarcoma of the bladder: a case report. J Urol. 1987 May;137(5):984–985. doi: 10.1016/s0022-5347(17)44323-0. [DOI] [PubMed] [Google Scholar]
  34. Swartz D. A., Johnson D. E., Ayala A. G., Watkins D. L. Bladder leiomyosarcoma: a review of 10 cases with 5-year followup. J Urol. 1985 Feb;133(2):200–202. doi: 10.1016/s0022-5347(17)48880-x. [DOI] [PubMed] [Google Scholar]
  35. Swenberg J. A., Koestner A., Wechsler W., Brunden M. N., Abe H. Differential oncogenic effects of methylnitrosourea. J Natl Cancer Inst. 1975 Jan;54(1):89–96. doi: 10.1093/jnci/54.1.89. [DOI] [PubMed] [Google Scholar]
  36. Turner A. G. Malignant fibrous histiocytoma involving the bladder. Br J Urol. 1985 Apr;57(2):237–238. doi: 10.1111/j.1464-410x.1985.tb06433.x. [DOI] [PubMed] [Google Scholar]
  37. VANBUSKIRK K. E., CLARK P. K., SNOGA J. R., ATTWOOD C. NEUROFIBROMA OF THE BLADDER: CASE REPORT AND REVIEW OF THE LITERATURE. J Urol. 1964 Mar;91:241–245. doi: 10.1016/s0022-5347(17)64099-0. [DOI] [PubMed] [Google Scholar]
  38. Ward J. M., Kulwich B. A., Reznik G., Berman J. J. Malignant fibrous histiocytoma. An unusual neoplasm of soft-tissue origin in the rat that is different from the human counterpart. Arch Pathol Lab Med. 1981 Jun;105(6):313–316. [PubMed] [Google Scholar]
  39. Winfield H. N., Catalona W. J. An isolated plexiform neurofibroma of the bladder. J Urol. 1985 Sep;134(3):542–543. doi: 10.1016/s0022-5347(17)47285-5. [DOI] [PubMed] [Google Scholar]
  40. Young R. H., Rosenberg A. E. Osteosarcoma of the urinary bladder. Report of a case and review of the literature. Cancer. 1987 Jan 1;59(1):174–178. doi: 10.1002/1097-0142(19870101)59:1<174::aid-cncr2820590133>3.0.co;2-v. [DOI] [PubMed] [Google Scholar]

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