Abstract
The present study was undertaken to examine changes in vascular ultrastructure of rats subjected to hypervitaminosis D with or without treatment with ethane-I-hydroxy-I, I-diphosphonate (EHDP). Five groups of rats were studied. Untreated rats were given 0.9% NaCl i.p. Sham-treated rats were given vehicle (corn oil). Treated rats were given ergocalciferol (75,000 IU i.p.) dissolved in vehicle with or without EHDP (5 mM/100 g body-weight i.p.). Rats which had been given ergocalciferol without EHDP developed hypercalcemia and demonstrated significant arterial calcinosis. A similar degree of calcinosis was not observed in rats given ergocalciferol with EHDP. EHDP appeared to inhibit arterial calcinosis; however, it did not affect plasma calcium levels. This suggests that EHDP might delay calcium influx into the cell and thereby prevent calcium overload. Our findings support the suggestion that EHDP therapy can be an effective treatment for the inhibition of dystrophic arterial calcinosis.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bonucci E. Fine structure and histochemistry of "calcifying globules" in epiphyseal cartilage. Z Zellforsch Mikrosk Anat. 1970;103(2):192–217. doi: 10.1007/BF00337312. [DOI] [PubMed] [Google Scholar]
- Daoud A. S., Frank A. S., Jarmolych J., Fritz K. E. The effect of ethane-1-hydroxy-1,1-diphosphonate (EHDP) on necrosis of atherosclerotic lesions. Atherosclerosis. 1987 Sep;67(1):41–48. doi: 10.1016/0021-9150(87)90263-2. [DOI] [PubMed] [Google Scholar]
- Guilland D. F., Fleisch H. The effect of in vivo treatment with EHDP and/or 1,25-DHCC on calcium uptake and release in isolated kidney mitochondria. Biochem Biophys Res Commun. 1974 Dec 11;61(3):906–911. doi: 10.1016/0006-291x(74)90241-1. [DOI] [PubMed] [Google Scholar]
- Haust M. D., Geer J. C. Mechanism of calcification in spontaneous aortic arteriosclerotic lesions of the rabbit. An electron microscopic study. Am J Pathol. 1970 Sep;60(3):329–346. [PMC free article] [PubMed] [Google Scholar]
- Hollander W., Paddock J., Nagraj S., Colombo M., Kirkpatrick B. Effects of anticalcifying and antifibrobrotic drugs on pre-established atherosclerosis in the rabbit. Atherosclerosis. 1979 May;33(1):111–123. doi: 10.1016/0021-9150(79)90202-8. [DOI] [PubMed] [Google Scholar]
- Levy R. J., Hawley M. A., Schoen F. J., Lund S. A., Liu P. Y. Inhibition by diphosphonate compounds of calcification of porcine bioprosthetic heart valve cusps implanted subcutaneously in rats. Circulation. 1985 Feb;71(2):349–356. doi: 10.1161/01.cir.71.2.349. [DOI] [PubMed] [Google Scholar]
- Potokar M., Schmidt-Dunker M. The inhibitory effect of new diphosphonic acids on aortic and kidney calcification in vivo. Atherosclerosis. 1978 Aug;30(4):313–320. doi: 10.1016/0021-9150(78)90124-7. [DOI] [PubMed] [Google Scholar]
- Schenk R., Merz W. A., Mühlbauer R., Russell R. G., Fleisch H. Effect of ethane-1-hydroxy-1,1-diphosphonate (EHDP) and dichloromethylene diphosphonate (Cl 2 MDP) on the calcification and resorption of cartilage and bone in the tibial epiphysis and metaphysis of rats. Calcif Tissue Res. 1973 Mar 12;11(3):196–214. doi: 10.1007/BF02547219. [DOI] [PubMed] [Google Scholar]
- Wagner W. D., Clarkson T. B., Foster J. Contrasting effects of ethane-1-hydroxy-1,1-diphosphonate (EHDP) on the regression of two types of dietary-induced atherosclerosis. Atherosclerosis. 1977 Aug;27(4):419–435. doi: 10.1016/0021-9150(77)90161-7. [DOI] [PubMed] [Google Scholar]
- Yellon D. M., Hearse D. J., Maxwell M. P., Chambers D. E., Downey J. M. Sustained limitation of myocardial necrosis 24 hours after coronary artery occlusion: verapamil infusion in dogs with small myocardial infarcts. Am J Cardiol. 1983 May 1;51(8):1409–1413. doi: 10.1016/0002-9149(83)90321-1. [DOI] [PubMed] [Google Scholar]