PERSPECTIVE. For the article “Single-Molecule Chemistry and Biology Special Feature: New directions in singlemolecule imaging and analysis,” by W. E. Moerner, which appeared in issue 31, July 31, 2007, of Proc Natl Acad Sci USA (104:12596–12602; first published July 30, 2007; 10.1073 /pnas.0610081104), the author notes that, due to a printer's error, Fig. 3A appeared incorrectly. The online version has been corrected. The corrected figure and its legend appear below. This error does not affect the conclusions of the article.
Fig. 3.
Overview of superresolution imaging. (A) Schematic of a tightly focused laser beam (blue) of diffraction-limited diameter ≈200 nm irradiating a cell. One molecule is in the focal volume, which emits fluorescence (red). (B) Wide-field fluorescence image of a bacterial cell (red) containing a single protein fusion between the bacterial actin MreB and EYFP (mountain). Acquisition time, 100 ms. (Scale bar, 0.5 μm.) (C) Fluorescence PALM image of PA-GFP molecules on a glass substrate, with green regions showing the approximate blur region of diffraction-limited imaging and yellow dots showing the actual detected positions of the single molecules. [Reproduced with permission from ref. 78 (Copyright 2006, Biophysical Society).] (D) Confocal (Left) and STED (Right) images of a neurofilament in a human neuroblastoma cell labeled by immunofluorescence. [Reproduced with permission from ref. 88 (Copyright 2006, National Academy of Sciences).]