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. 1986 Feb;53(2):217–222. doi: 10.1038/bjc.1986.38

Quantitative cytospectrophotometric studies on protein thiols and reactive protein disulphides in samples of normal human uterine cervix and on samples obtained from patients with dysplasia or carcinoma-in-situ.

G Nöhammer, F Bajardi, C Benedetto, E Schauenstein, T F Slater
PMCID: PMC2001329  PMID: 3954943

Abstract

Quantitative microspectophotometric studies have been made on sections of human cervix after staining for reactive protein thiol-groups (PSHr), and the sum of protein thiols with so-called reactive protein disulphides (together abbreviated as TRPS). Measurements were made on normal epithelium, apparently normal epithelium adjacent to a pathological lesion, dysplastic epithelium, carcinoma-in-situ, and adjoining stroma. The numbers of cases studied were: normal healthy controls (53); patients with dysplasias (34) and patients with carcinoma-in-situ (29). In the normal control sections the ratio of PSHr in epithelium:stroma was approximately 2.7 and this ratio was strongly decreased in dysplasias (1.6) and carcinoma-in-situ (1.5); the 3 populations of values had sufficient overlap to prevent this measurement being an effective discriminator. No significant variations were observed with TRPS-values except with changes in the stroma adjacent to apparently normal epithelium. However, the ratio of PSHr:TRPS was effectively discriminatory when this double-staining ratio was calculated for epithelial values:stromal values. These results are discussed in relation to the importance of thiol-groups in cell division and cancer, and the biological implications of similar changes observed in neighbouring apparently normal epithelium.

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Selected References

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  1. Araki T., Nakae Y., Chikamori K., Katsura S., Yamada M. O. Micro-assay of sulfhydryl group content per tissue protein by tridensitometry. Cell Mol Biol. 1982;28(2):213–216. [PubMed] [Google Scholar]
  2. Aurelian L., Kessler I. I., Rosenshein N. B., Barbour G. Viruses and gynecologic cancers: herpesvirus protein (ICP 10/AG-4), a cervical tumor antigen that fulfills the criteria for a marker of carcinogenicity. Cancer. 1981 Jul 15;48(2 Suppl):455–471. doi: 10.1002/1097-0142(19810715)48:1+<455::aid-cncr2820481306>3.0.co;2-2. [DOI] [PubMed] [Google Scholar]
  3. Bajardi F., Benedetto C., Nöhammer G., Schauenstein E., Slater T. F. Histophotometrical investigations on the content of protein and protein thiols in the epithelium and stroma of the human uterine cervix. II. Intraepithelial neoplasia. Histochemistry. 1983;78(1):95–100. doi: 10.1007/BF00491115. [DOI] [PubMed] [Google Scholar]
  4. Bajardi F., Jüttner F., Smolle J. Korrespondierende Verhaltensweisen von Epithel und Stroma der Cervix uteri. Zentralbl Gynakol. 1983;105(5):257–264. [PubMed] [Google Scholar]
  5. Benedetto C., Bocci A., Dianzani M. U., Ghiringhello B., Slater T. F., Tomasi A., Vannini V. Electron spin resonance studies on normal human uterus and cervix and on benign and malignant uterine tumors. Cancer Res. 1981 Jul;41(7):2936–2942. [PubMed] [Google Scholar]
  6. Crum C. P., Ikenberg H., Richart R. M., Gissman L. Human papillomavirus type 16 and early cervical neoplasia. N Engl J Med. 1984 Apr 5;310(14):880–883. doi: 10.1056/NEJM198404053101403. [DOI] [PubMed] [Google Scholar]
  7. Harington J. S. The sulfhydryl group and carcinogenesis. Adv Cancer Res. 1967;10:247–309. doi: 10.1016/s0065-230x(08)60080-9. [DOI] [PubMed] [Google Scholar]
  8. Knock F. E., Galt R. M., Oester Y. T. Protein-sulfhydryl groups in cellular control mechanisms and cancer. J Am Geriatr Soc. 1967 Oct;15(10):882–899. doi: 10.1111/j.1532-5415.1967.tb02519.x. [DOI] [PubMed] [Google Scholar]
  9. Kosower N. S., Kosower E. M. The glutathione status of cells. Int Rev Cytol. 1978;54:109–160. doi: 10.1016/s0074-7696(08)60166-7. [DOI] [PubMed] [Google Scholar]
  10. Mannervik B., Axelsson K. Role of cytoplasmic thioltransferase in cellular regulation by thiol-disulphide interchange. Biochem J. 1980 Jul 15;190(1):125–130. doi: 10.1042/bj1900125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Millett J. A., Husain O. A., Bitensky L., Chayen J. Feulgen-hydrolysis profiles in cells exfoliated from the cervix uteri: a potential aid in the diagnosis of malignancy. J Clin Pathol. 1982 Mar;35(3):345–349. doi: 10.1136/jcp.35.3.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nöhammer G. Quantitative microspectrophotometrical determination of protein thiols and disulfides with 2,2'-dihydroxy-6,6'-dinaphthyldisulfide (DDD). The variety of DDD-staining methods demonstrated on Ehrlich ascites tumor cells. Histochemistry. 1982;75(2):219–250. doi: 10.1007/BF00496014. [DOI] [PubMed] [Google Scholar]
  13. Schauenstein E., Bajardi F., Benedetto C., Nöhammer G., Slater T. F. Histophotometrical investigations on the contents of protein and protein thiols of the epithelium and stroma of the human cervix. I. Cases with no apparent neoplastic alterations of the epithelium. Histochemistry. 1983;77(4):465–472. doi: 10.1007/BF00495801. [DOI] [PubMed] [Google Scholar]
  14. Sherbet G. V. The cell surface sulphydryl content of metastatic variants of B16 murine melanoma. Exp Cell Biol. 1983;51(3):140–147. doi: 10.1159/000163184. [DOI] [PubMed] [Google Scholar]
  15. Slater T. F., Bajardi F., Benedetto C., Bussolati G., Cianfano S., Dianzani M. U., Ghiringhello B., Nöhammer G., Rojanapo W., Schauenstein E. Protein thiols in normal and neoplastic human uterine cervix. FEBS Lett. 1985 Aug 5;187(2):267–271. doi: 10.1016/0014-5793(85)81256-4. [DOI] [PubMed] [Google Scholar]

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