Abstract
Experiments were performed on cultured Chinese hamster ovary cells exposed to haematoporphyrin derivative (HpD) plus light, yielding survival rates of 40-100%. [3H]-thymidine, [3H]-tryptophan and [14C]-lysine incorporation were used to quantitate DNA and protein synthesis in surviving cells after exposure. Multiple experiments demonstrated 78% reduction in DNA synthesis during the first day after exposure to 20 micrograms ml-1 HpD plus 1140 Jm-2 light followed by progressive recovery to the normal rate after 4-6 days. Protein synthesis was somewhat less sensitive dropping by 54% initially and fully recovering by day 4. Although this cell line has a normal cycle time averaging approximately 15 h, cell division was rarely observed among lone surviving cells until 72 h after exposure. No inhibition was observed in cells exposed to HpD in the dark. These results indicate that photoactivated HpD has a wide spectrum of reversible nuclear and cytoplasmic effects even at sublethal doses. This is consistent with the notion that clinical photodynamic therapy is not likely to result in chronic morbidity.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bellnier D. A., Dougherty T. J. Membrane lysis in Chinese hamster ovary cells treated with hemtoporphyrin derivative plus light. Photochem Photobiol. 1982 Jul;36(1):43–47. doi: 10.1111/j.1751-1097.1982.tb04338.x. [DOI] [PubMed] [Google Scholar]
- Christensen T., Feren K., Moan J., Pettersen E. Photodynamic effects of haematoporphyrin derivative on synchronized and asynchronous cells of different origin. Br J Cancer. 1981 Nov;44(5):717–724. doi: 10.1038/bjc.1981.258. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Christensen T. Multiplication of human NHIK 3025 cells exposed to porphyrins in combination with light. Br J Cancer. 1981 Sep;44(3):433–439. doi: 10.1038/bjc.1981.202. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dougherty T. J. Photoradiation therapy. Urology. 1984 Mar;23(3 Suppl):61–64. doi: 10.1016/s0090-4295(84)80119-3. [DOI] [PubMed] [Google Scholar]
- Evensen J. F., Moan J. Photodynamic action and chromosomal damage: a comparison of haematoporphyrin derivative (HpD) and light with X-irradiation. Br J Cancer. 1982 Mar;45(3):456–465. doi: 10.1038/bjc.1982.74. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gibson S. L., Hilf R. Photosensitization of mitochondrial cytochrome c oxidase by hematoporphyrin derivative and related porphyrins in vitro and in vivo. Cancer Res. 1983 Sep;43(9):4191–4197. [PubMed] [Google Scholar]
- Gomer C. J., Rucker N., Banerjee A., Benedict W. F. Comparison of mutagenicity and induction of sister chromatid exchange in Chinese hamster cells exposed to hematoporphyrin derivative photoradiation, ionizing radiation, or ultraviolet radiation. Cancer Res. 1983 Jun;43(6):2622–2627. [PubMed] [Google Scholar]
- LIPSON R. L., BALDES E. J., OLSEN A. M. The use of a derivative of hematoporhyrin in tumor detection. J Natl Cancer Inst. 1961 Jan;26:1–11. [PubMed] [Google Scholar]
- Moan J., Johannessen J. V., Christensen T., Espevik T., McGhie J. B. Porphyrin-sensitized photoinactivation of human cells in vitro. Am J Pathol. 1982 Nov;109(2):184–192. [PMC free article] [PubMed] [Google Scholar]
- Moan J., McGhie J., Jacobsen P. B. Photodynamic effects on cells in vitro exposed to hematoporphyrin derivative and light. Photochem Photobiol. 1983 Jun;37(6):599–604. doi: 10.1111/j.1751-1097.1983.tb04527.x. [DOI] [PubMed] [Google Scholar]
- Moan J., Pettersen E. O., Christensen T. The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin. Br J Cancer. 1979 Apr;39(4):398–407. doi: 10.1038/bjc.1979.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moan J., Waksvik H., Christensen T. DNA single-strand breaks and sister chromatid exchanges induced by treatment with hematoporphyrin and light or by x-rays in human NHIK 3025 cells. Cancer Res. 1980 Aug;40(8 Pt 1):2915–2918. [PubMed] [Google Scholar]
- Weishaupt K. R., Gomer C. J., Dougherty T. J. Identification of singlet oxygen as the cytotoxic agent in photoinactivation of a murine tumor. Cancer Res. 1976 Jul;36(7 Pt 1):2326–2329. [PubMed] [Google Scholar]