Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1986 Dec;54(6):989–998. doi: 10.1038/bjc.1986.271

Kinetic studies on a murine sarcoma and an analysis of apoptosis.

C E Sarraf, I D Bowen
PMCID: PMC2001609  PMID: 3801293

Abstract

A stathmokinetic method has been used to determine the cell cycle parameters, particularly the potential tumour doubling time, of a murine fat pad sarcoma. Additional information has been obtained by determining the percentage of labelled mitoses (PLM). A technique which simultaneously demonstrates autoradiographically labelled S phase nuclei and histochemically localized acid phosphatase activity has also been used at light microscope level to compare these parameters: acid phosphatase activity was demonstrated in tumour cells and macrophages. Single cell deletion by apoptosis has been investigated as distinct from necrosis. Condensed, dying apoptotic cells, have been found in proliferative areas of tumour that are not under physiological stress. The analysis of apoptosis indicated a previously unsuspected variation in apoptotic activity with tumour weight. Cell death by apoptosis initially rose as the tumour grew, but after the tumour reached a threshold weight it declined dramatically, and finally remained stable. This may reflect an initial attempt at autoregulation of tumour size which ultimately fails. Apoptosis was estimated to account for an average of 7% of the total cell loss rate in this tumour.

Full text

PDF
989

Images in this article

991Figure 1
on p.991
992Figure 2
on p.992
992Figure 3
on p.992
992Figure 4
on p.992

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aherne W. A., Challoner D. A reappraisal of the stathmokinetic technique. II. Metaphase degeneration and its correction. Virchows Arch B Cell Pathol Incl Mol Pathol. 1983;42(1):111–118. doi: 10.1007/BF02890374. [DOI] [PubMed] [Google Scholar]
  2. Bellamy D., Hinsull S. M. Density-dependent mitosis in the Walker 256 carcinoma and the influence of host age on growth. Eur J Cancer. 1978 Jul;14(7):747–751. doi: 10.1016/0014-2964(78)90003-8. [DOI] [PubMed] [Google Scholar]
  3. Bellamy D., Hinsull S. M., Phillips J. G. Factors controlling growth and age involution of the rat thymus. Age Ageing. 1976 Feb;5(1):12–19. doi: 10.1093/ageing/5.1.12. [DOI] [PubMed] [Google Scholar]
  4. Bowen I. D., Lewis G. H. Acid phosphatase activity and cell death in mouse thymus. Histochemistry. 1980 Feb;65(2):173–179. doi: 10.1007/BF00493166. [DOI] [PubMed] [Google Scholar]
  5. Carswell E. A., Old L. J., Kassel R. L., Green S., Fiore N., Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3666–3670. doi: 10.1073/pnas.72.9.3666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Columbano A., Ledda-Columbano G. M., Rao P. M., Rajalakshmi S., Sarma D. S. Occurrence of cell death (apoptosis) in preneoplastic and neoplastic liver cells. A sequential study. Am J Pathol. 1984 Sep;116(3):441–446. [PMC free article] [PubMed] [Google Scholar]
  7. Denekamp J. Vascular endothelium as the vulnerable element in tumours. Acta Radiol Oncol. 1984;23(4):217–225. doi: 10.3109/02841868409136015. [DOI] [PubMed] [Google Scholar]
  8. Kerr J. F., Wyllie A. H., Currie A. R. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer. 1972 Aug;26(4):239–257. doi: 10.1038/bjc.1972.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lewis G. H., Bowen I. D. A methacrylate embedding technique for combined autoradiography and acid phosphatase histochemistry. Histochem J. 1985 Apr;17(4):467–475. doi: 10.1007/BF01003206. [DOI] [PubMed] [Google Scholar]
  10. Loewenstein J., Gallily R. Studies on the mechanism of macrophage-mediated tumor cell lysis induced by Mycoplasma orale. Isr J Med Sci. 1984 Sep;20(9):895–897. [PubMed] [Google Scholar]
  11. MENDELSOHN M. L. Autoradiographic analysis of cell proliferation in spontaneous breast cancer of C3H mouse. I. Growth and survival of cells labeled with tritiated thymidine. J Natl Cancer Inst. 1960 Sep;25:485–500. [PubMed] [Google Scholar]
  12. PUCK T. T., STEFFEN J. LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. I. A METHOD FOR LOCALIZING METABOLIC EVENTS WITHIN THE LIFE CYCLE, AND ITS APPLICATION TO THE ACTION OF COLCEMIDE AND SUBLETHAL DOSES OF X-IRRADIATION. Biophys J. 1963 Sep;3:379–397. doi: 10.1016/s0006-3495(63)86828-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Russell J. H., Masakowski V., Rucinsky T., Phillips G. Mechanisms of immune lysis. III. Characterization of the nature and kinetics of the cytotoxic T lymphocyte-induced nuclear lesion in the target. J Immunol. 1982 May;128(5):2087–2094. [PubMed] [Google Scholar]
  14. Searle J., Collins D. J., Harmon B., Kerr J. F. The spontaneous occurrence of apoptosis in squamous carcinomas of the uterine cervix. Pathology. 1973 Apr;5(2):163–169. doi: 10.3109/00313027309060831. [DOI] [PubMed] [Google Scholar]
  15. Sylvén B., Niemi M. Histochemical evidence of cell death in transplanted tumours. Virchows Arch B Cell Pathol. 1972;10(2):127–133. doi: 10.1007/BF02899722. [DOI] [PubMed] [Google Scholar]
  16. Tannock I. F. Population kinetics of carcinoma cells, capillary endothelial cells, and fibroblasts in a transplanted mouse mammary tumor. Cancer Res. 1970 Oct;30(10):2470–2476. [PubMed] [Google Scholar]
  17. Tannock I. F. The relation between cell proliferation and the vascular system in a transplanted mouse mammary tumour. Br J Cancer. 1968 Jun;22(2):258–273. doi: 10.1038/bjc.1968.34. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WEAVER J. A. Changes induced in the thymus and lymph nodes of the rat by the administration of cortisone and sex hormones and by other procedures. J Pathol Bacteriol. 1955 Jan-Apr;69(1-2):133–139. doi: 10.1002/path.1700690119. [DOI] [PubMed] [Google Scholar]
  19. Weinberg J. B., Haney A. F. Spontaneous tumor cell killing by human blood monocytes and human peritoneal macrophages: lack of alteration by endotoxin or quenchers of reactive oxygen species. J Natl Cancer Inst. 1983 Jun;70(6):1005–1010. [PubMed] [Google Scholar]
  20. Wright N. A., Appleton D. R. The metaphase arrest technique. A critical review. Cell Tissue Kinet. 1980 Nov;13(6):643–663. doi: 10.1111/j.1365-2184.1980.tb00503.x. [DOI] [PubMed] [Google Scholar]
  21. Wyllie A. H. Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature. 1980 Apr 10;284(5756):555–556. doi: 10.1038/284555a0. [DOI] [PubMed] [Google Scholar]
  22. Wyllie A. H., Morris R. G., Smith A. L., Dunlop D. Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis. J Pathol. 1984 Jan;142(1):67–77. doi: 10.1002/path.1711420112. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES