Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1987 Jul;56(1):45–48. doi: 10.1038/bjc.1987.150

Cholestyramine promotes 7,12-dimethylbenzanthracene induced mammary cancer in Wistar rats.

M F Melhem, H F Gabriel, E D Eskander, K N Rao
PMCID: PMC2001672  PMID: 3113472

Abstract

The promotion of 7,12-dimethylbenzanthracene (DMBA) induced mammary cancer in Wistar rats by a 4% cholestyramine (CHST) diet was investigated. The rats, 50 days of age, were divided into six groups. First two groups were given an intragastric dose of 0.8 ml of corn oil whereas the remaining four groups were given a single intragastric dose of 5 mg of DMBA dissolved in 0.8 ml of corn oil. After 1 week on laboratory chow the first two groups and two groups treated with DMBA were fed a control diet and the two remaining groups treated with DMBA were fed a 4% CHST diet. Half the animals were killed at 100 days and the remainder at 200 days. A detailed histologic examination of grossly normal mammary tissue as well as any tumour mass was made for each rat. The serum lipids were extracted and the individual neutral lipid composition was determined. In rats treated with DMBA and fed a 4% CHST diet, the incidence of malignant tumours increased by 5 fold, and the tumour weight by 12 fold. In addition, the serum total lipids, cholesterol esters and triglycerides decreased significantly when compared with rats fed a control diet. These results suggest that CHST diet promotes DMBA induced mammary cancers in Wistar rats.

Full text

PDF
45

Images in this article

47Figure 1
on p.47

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen J. M., Dietschy J. M. Regulation of sterol synthesis in 15 tissues of rat. II. Role of rat and human high and low density plasma lipoproteins and of rat chylomicron remnants. J Biol Chem. 1977 Jun 10;252(11):3652–3659. [PubMed] [Google Scholar]
  2. Asano T., Pollard M., Madsen D. C. Effects of cholestyramine on 1,2-dimethylhydrazine-induced enteric carcinoma in germfree rats. Proc Soc Exp Biol Med. 1975 Dec;150(3):780–785. doi: 10.3181/00379727-150-39124. [DOI] [PubMed] [Google Scholar]
  3. Beuving L. J., Faulkin L. J., Jr, DeOme K. B., Bergs V. V. Hyperplastic lesions in the mammary glands of Sprague-Dawley rats after 7,12-dimethyl-benz[a]anthracene treatment. J Natl Cancer Inst. 1967 Sep;39(3):423–429. [PubMed] [Google Scholar]
  4. Brand S. J., Morgan R. G. Stimulation of pancreatic secretion and growth in the rat after feeding cholestyramine. Gastroenterology. 1982 Oct;83(4):851–859. [PubMed] [Google Scholar]
  5. Brown M. S., Kovanen P. T., Goldstein J. L. Regulation of plasma cholesterol by lipoprotein receptors. Science. 1981 May 8;212(4495):628–635. doi: 10.1126/science.6261329. [DOI] [PubMed] [Google Scholar]
  6. Cohen L. A., Thompson D. O., Choi K., Karmali R. A., Rose D. P. Dietary fat and mammary cancer. II. Modulation of serum and tumor lipid composition and tumor prostaglandins by different dietary fats: association with tumor incidence patterns. J Natl Cancer Inst. 1986 Jul;77(1):43–51. [PubMed] [Google Scholar]
  7. Coleman P. S., Lavietes B. B. Membrane cholesterol, tumorigenesis, and the biochemical phenotype of neoplasia. CRC Crit Rev Biochem. 1981;11(4):341–393. doi: 10.1080/10409238109104421. [DOI] [PubMed] [Google Scholar]
  8. Graham S. Dietary factors in the prevention of cancer. Transplant Proc. 1984 Apr;16(2):392–400. [PubMed] [Google Scholar]
  9. HUFF J. W., GILFILLAN J. L., HUNT V. M. EFFECT OF CHOLESTYRAMINE, A BILE ACID-BINDING POLYMER ON PLASMA CHOLESTEROL AND FECAL BILE ACID EXCRETION IN THE RAT. Proc Soc Exp Biol Med. 1963 Nov;114:352–355. doi: 10.3181/00379727-114-28674. [DOI] [PubMed] [Google Scholar]
  10. Nigro N. D., Bhadrachari N., Chomchai C. A rat model for studying colonic cancer: effect of cholestyramine on induced tumors. Dis Colon Rectum. 1973 Nov-Dec;16(6):438–443. doi: 10.1007/BF02588866. [DOI] [PubMed] [Google Scholar]
  11. Pani P., Dessi S., Rao K. N., Batetta B., Laconi E. Changes in serum and hepatic cholesterol in lead-induced liver hyperplasia. Toxicol Pathol. 1984;12(2):162–167. doi: 10.1177/019262338401200208. [DOI] [PubMed] [Google Scholar]
  12. Ramesha C. S., Paul R., Ganguly J. Effect of dietary unsaturated oils on the biosynthesis of cholesterol, and on biliary and fecal excretion of cholesterol and bile acids in rats. J Nutr. 1980 Nov;110(11):2149–2158. doi: 10.1093/jn/110.11.2149. [DOI] [PubMed] [Google Scholar]
  13. Rao K. N., Katyal S. L., Iammarino R. M., Lombardi B. Acute hemorrhagic pancreatic necrosis in mice. Alterations of pancreatic lipase activity, pancreas lipids, and serum lipoproteins. Digestion. 1980;20(5):314–322. doi: 10.1159/000198453. [DOI] [PubMed] [Google Scholar]
  14. Rao K. N., Kottapally S., Eskander E. D., Shinozuka H., Dessi S., Pani P. Acinar cell carcinoma of rat pancreas: regulation of cholesterol esterification. Br J Cancer. 1986 Aug;54(2):305–310. doi: 10.1038/bjc.1986.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rao K. N., Kottapally S., Shinozuka H. Acinar cell carcinoma of rat pancreas: mechanism of deregulation of cholesterol metabolism. Toxicol Pathol. 1984;12(1):62–68. doi: 10.1177/019262338401200110. [DOI] [PubMed] [Google Scholar]
  16. Rao K. N., Kottapally S., Shinozuka H. Lipid composition and 3-hydroxy-3-methylglutaryl-CoA reductase activity of acinar cell carcinoma of rat pancreas. Biochim Biophys Acta. 1983 Aug 23;759(1-2):74–80. doi: 10.1016/0304-4165(83)90191-5. [DOI] [PubMed] [Google Scholar]
  17. Rao K. N., Shinozuka H., Kunz H. W., Gill T. J., 3rd Enhanced susceptibility to a chemical carcinogen in rats carrying MHC-linked genes influencing development (GRC). Int J Cancer. 1984 Jul 15;34(1):113–120. doi: 10.1002/ijc.2910340120. [DOI] [PubMed] [Google Scholar]
  18. Report of the American Institute of Nurtition ad hoc Committee on Standards for Nutritional Studies. J Nutr. 1977 Jul;107(7):1340–1348. doi: 10.1093/jn/107.7.1340. [DOI] [PubMed] [Google Scholar]
  19. Rogers A. E., Conner B., Boulanger C., Lee S. Mammary tumorigenesis in rats fed diets high in lard. Lipids. 1986 Apr;21(4):275–280. doi: 10.1007/BF02536412. [DOI] [PubMed] [Google Scholar]
  20. Siperstein M. D. Role of cholesterogenesis and isoprenoid synthesis in DNA replication and cell growth. J Lipid Res. 1984 Dec 15;25(13):1462–1468. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES