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. 1987 Apr;55(4):421–426. doi: 10.1038/bjc.1987.82

Characterization of factor XIII containing-macrophages in lymph nodes with Hodgkin's disease.

R Adány, Z Nemes, L Muszbek
PMCID: PMC2001696  PMID: 3555591

Abstract

A large number of cells containing subunit a of blood coagulation Factor XIII (FXIII) was detected by immunoperoxidase staining in lymph nodes with Hodgkin's disease. These relatively large, multipolar, mononuclear cells were often found in the immediate vicinity of malignant Hodgkin's cells. Intensive characterization of these cells carried out by immunofluorescent and enzymecytochemical techniques in double- and triple-labelling systems on the same sections clearly demonstrated that they represent tumour-associated macrophages (TAMs). FXIII containing-cells showed alpha-naphtyl acetate esterase (ANAE) positivity, and were labelled by monoclonal anti-Leu M3 antibody, a monocyte/macrophage marker, but not at all or only very weakly by anti-HLA-DR. Neither alkaline phosphatase (ALP) nor adenosine triphosphatase (ATPase) activity could be detected in these cells and surprisingly, they were consistently negative for acid phosphatase (AcP) as well. The presence of FXIII subunit a in tumour-associated macrophages suggests that this cell type might have an important role in the stabilization of fibrin deposits around tumour cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adány R., Belkin A., Vasilevskaya T., Muszbek L. Identification of blood coagulation factor XIII in human peritoneal macrophages. Eur J Cell Biol. 1985 Jul;38(1):171–173. [PubMed] [Google Scholar]
  2. BECK E., DUCKERT F., ERNST M. The influence of fibrin stabilizing factor on the growth of fibroblasts in vitro and wound healing. Thromb Diath Haemorrh. 1961 Dec 15;6:485–491. [PubMed] [Google Scholar]
  3. Bohn H., Schwick H. G. Isolierung und Charakterisierung eines fibrinstabilisierenden Faktors aus menschlichen Plazenten. Arzneimittelforschung. 1971 Oct;21(10):1432–1439. [PubMed] [Google Scholar]
  4. Chapman H. A., Jr, Allen C. L., Stone O. L., Fair D. S. Human alveolar macrophages synthesize factor VII in vitro. Possible role in interstitial lung disease. J Clin Invest. 1985 Jun;75(6):2030–2037. doi: 10.1172/JCI111922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chilosi M., Pizzolo G., Menestrina F., Iannucci A. M., Bonetti F., Fiore-Donati L. Enzyme histochemistry on normal and pathologic paraffin-embedded lymphoid tissues. Am J Clin Pathol. 1981 Dec;76(6):729–736. doi: 10.1093/ajcp/76.6.729. [DOI] [PubMed] [Google Scholar]
  6. Dimitriu-Bona A., Burmester G. R., Waters S. J., Winchester R. J. Human mononuclear phagocyte differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol. 1983 Jan;130(1):145–152. [PubMed] [Google Scholar]
  7. Duckert F. Documentation of the plasma factor XIII deficiency in man. Ann N Y Acad Sci. 1972 Dec 8;202:190–199. doi: 10.1111/j.1749-6632.1972.tb16331.x. [DOI] [PubMed] [Google Scholar]
  8. Dvorak H. F., Senger D. R., Dvorak A. M. Fibrin as a component of the tumor stroma: origins and biological significance. Cancer Metastasis Rev. 1983;2(1):41–73. doi: 10.1007/BF00046905. [DOI] [PubMed] [Google Scholar]
  9. Edwards R. L., Rickles F. R., Cronlund M. Abnormalities of blood coagulation in patients with cancer. Mononuclear cell tissue factor generation. J Lab Clin Med. 1981 Dec;98(6):917–928. [PubMed] [Google Scholar]
  10. Evans R. Macrophages and neoplasms: new insights and their implication in tumor immunobiology. Cancer Metastasis Rev. 1982;1(3):227–239. doi: 10.1007/BF00046829. [DOI] [PubMed] [Google Scholar]
  11. Gordon S. G., Cross B. A. A factor X-activating cysteine protease from malignant tissue. J Clin Invest. 1981 Jun;67(6):1665–1671. doi: 10.1172/JCI110203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gordon S. G., Franks J. J., Lewis B. J. Comparison of procoagulant activities in extracts of normal and malignant human tissue. J Natl Cancer Inst. 1979 Apr;62(4):773–776. [PubMed] [Google Scholar]
  13. Gordon S. G., Franks J. J., Lewis B. Cancer procoagulant A: a factor X activating procoagulant from malignant tissue. Thromb Res. 1975 Feb;6(2):127–137. doi: 10.1016/0049-3848(75)90018-3. [DOI] [PubMed] [Google Scholar]
  14. Hansmann M. L., Kaiserling E. Electron-microscopic aspects of Hodgkin's disease. J Cancer Res Clin Oncol. 1981;101(1):135–148. doi: 10.1007/BF00405074. [DOI] [PubMed] [Google Scholar]
  15. Harris N. L., Dvorak A. M., Smith J., Dvorak H. F. Fibrin deposits in Hodgkin's disease. Am J Pathol. 1982 Jul;108(1):119–129. [PMC free article] [PubMed] [Google Scholar]
  16. Henriksson P., Becker S., Lynch G., McDonagh J. Identification of intracellular factor XIII in human monocytes and macrophages. J Clin Invest. 1985 Aug;76(2):528–534. doi: 10.1172/JCI112002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hopper K. E., Wood P. R., Nelson D. S. Macrophage heterogeneity. Vox Sang. 1979;36(5):257–274. doi: 10.1111/j.1423-0410.1979.tb04434.x. [DOI] [PubMed] [Google Scholar]
  18. Hunyadi J., Szegedi G., Szabo T., Ahmed A., Laki K. Increased cytotoxic sensitivity of YPC-1 tumor cells from mice treated with nitrosoureas. Cancer Res. 1981 May;41(5):1677–1681. [PubMed] [Google Scholar]
  19. Janossy G., Thomas J. A., Pizzolo G., Granger S. M., McLaughlin J., Habeshaw J. A., Stansfeld A. G., Sloane J. Immuno-histological diagnosis of lymphoproliferative diseases by selected combinations of antisera and monoclonal antibodies. Br J Cancer. 1980 Aug;42(2):224–242. doi: 10.1038/bjc.1980.222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Key M. E. Macrophages in cancer metastases and their relevance to metastatic growth. Cancer Metastasis Rev. 1983;2(1):75–88. doi: 10.1007/BF00046906. [DOI] [PubMed] [Google Scholar]
  21. Kiesselbach T. H., Wagner R. H. Demonstration of factor XIII in human megakaryocytes by a fluorescent antibody technique. Ann N Y Acad Sci. 1972 Dec 8;202:318–328. doi: 10.1111/j.1749-6632.1972.tb16344.x. [DOI] [PubMed] [Google Scholar]
  22. Lindahl U., Kolset S. O., Bøgwald J., Osterud B., Seljelid R. Studies, with a luminogenic peptide substrate, on blood coagulation factor X/Xa produced by mouse peritoneal macrophages. Biochem J. 1982 Aug 15;206(2):231–237. doi: 10.1042/bj2060231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Lorenzet R., Peri G., Locati D., Allavena P., Colucci M., Semeraro N., Mantovani A., Donati M. B. Generation of procoagulant activity by mononuclear phagocytes: a possible mechanism contributing to blood clotting activation within malignant tissues. Blood. 1983 Aug;62(2):271–273. [PubMed] [Google Scholar]
  24. Mueller J., Brun del Re G., Buerki H., Keller H. U., Hess M. W., Cottier H. Nonspecific acid esterase activity: a criterion for differentiation of T and B lymphocytes in mouse lymph nodes. Eur J Immunol. 1975 Apr;5(4):270–274. doi: 10.1002/eji.1830050411. [DOI] [PubMed] [Google Scholar]
  25. Muszbek L., Adány R., Szegedi G., Polgár J., Kávai M. Factor XIII of blood coagulation in human monocytes. Thromb Res. 1985 Feb 1;37(3):401–410. doi: 10.1016/0049-3848(85)90069-6. [DOI] [PubMed] [Google Scholar]
  26. Nemes Z., Thomázy V., Adány R., Muszbek L. Identification of histiocytic reticulum cells by the immunohistochemical demonstration of factor XIII (F-XIIIa) in human lymph nodes. J Pathol. 1986 Jun;149(2):121–132. doi: 10.1002/path.1711490207. [DOI] [PubMed] [Google Scholar]
  27. Osterud B., Lindahl U., Seljelid R. Macrophages produce blood coagulation factors. FEBS Lett. 1980 Oct 20;120(1):41–43. doi: 10.1016/0014-5793(80)81041-6. [DOI] [PubMed] [Google Scholar]
  28. Rickles F. R., Edwards R. L. Activation of blood coagulation in cancer: Trousseau's syndrome revisited. Blood. 1983 Jul;62(1):14–31. [PubMed] [Google Scholar]
  29. Schlager S. I., Dray S. Complete local tumor regression with antibody to fibrin Fragment E. J Immunol. 1975 Oct;115(4):976–981. [PubMed] [Google Scholar]
  30. Stein H., Bonk A., Tolksdorf G., Lennert K., Rodt H., Gerdes J. Immunohistologic analysis of the organization of normal lymphoid tissue and non-Hodgkin's lymphomas. J Histochem Cytochem. 1980 Aug;28(8):746–760. doi: 10.1177/28.8.7003001. [DOI] [PubMed] [Google Scholar]
  31. Stiller D., Katenkamp D. Intercellular substances in Hodgkin's lymphomas. Ultrastructural investigations. Virchows Arch A Pathol Anat Histol. 1978 Oct 18;380(1):81–90. doi: 10.1007/BF00432894. [DOI] [PubMed] [Google Scholar]
  32. van Dam-Mieras M. C., Muller A. D., van Deijk W. A., Hemker H. C. Clotting factors secreted by monocytes and macrophages: analytical considerations. Thromb Res. 1985 Jan 1;37(1):9–19. doi: 10.1016/0049-3848(85)90028-3. [DOI] [PubMed] [Google Scholar]

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