Abstract
To investigate the role of interleukin-1 beta (IL-1 beta) in glomerulonephritis we studied the presence of IL-1 beta by in-situ hybridization in in-situ immune complex glomerulonephritis in the rat. Glomerulonephritis was induced in preimmunized rats by unilateral renal perfusion with cationized human IgG. In-situ hybridization was performed on frozen sections with a battery of four 30mer oligonucleotide DNA probes 3' end labelled with 35S-dATP. IL-1 beta mRNA was detectable in nephritic glomeruli at 6 and 24 hours after induction of glomerulonephritis. Signal was maximal at 48 hours and markedly reduced by 4 days. The peak of IL-1 beta transcription coincided with the major monocyte influx into glomeruli consistent with a role for IL-1 beta as a mediator of glomerular hypercellularity.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boswell J. M., Yui M. A., Burt D. W., Kelley V. E. Increased tumor necrosis factor and IL-1 beta gene expression in the kidneys of mice with lupus nephritis. J Immunol. 1988 Nov 1;141(9):3050–3054. [PubMed] [Google Scholar]
- Boswell J. M., Yui M. A., Endres S., Burt D. W., Kelley V. E. Novel and enhanced IL-1 gene expression in autoimmune mice with lupus. J Immunol. 1988 Jul 1;141(1):118–124. [PubMed] [Google Scholar]
- Cook H. T., Smith J., Salmon J. A., Cattell V. Functional characteristics of macrophages in glomerulonephritis in the rat. O2- generation, MHC class II expression, and eicosanoid synthesis. Am J Pathol. 1989 Feb;134(2):431–437. [PMC free article] [PubMed] [Google Scholar]
- Diamond J. R., Pesek I. Glomerular tumor necrosis factor and interleukin 1 during acute aminonucleoside nephrosis. An immunohistochemical study. Lab Invest. 1991 Jan;64(1):21–28. [PubMed] [Google Scholar]
- Dinarello C. A., Wolff S. M. The role of interleukin-1 in disease. N Engl J Med. 1993 Jan 14;328(2):106–113. doi: 10.1056/NEJM199301143280207. [DOI] [PubMed] [Google Scholar]
- Lan H. Y., Nikolic-Paterson D. J., Zarama M., Vannice J. L., Atkins R. C. Suppression of experimental crescentic glomerulonephritis by the interleukin-1 receptor antagonist. Kidney Int. 1993 Feb;43(2):479–485. doi: 10.1038/ki.1993.70. [DOI] [PubMed] [Google Scholar]
- Lovett D. H., Ryan J. L., Sterzel R. B. Stimulation of rat mesangial cell proliferation by macrophage interleukin 1. J Immunol. 1983 Dec;131(6):2830–2836. [PubMed] [Google Scholar]
- Lovett D. H., Sterzel R. B., Ryan J. L., Atkins E. Production of an endogenous pyrogen by glomerular mesangial cells. J Immunol. 1985 Feb;134(2):670–672. [PubMed] [Google Scholar]
- Matsumoto K., Hatano M. Production of interleukin 1 in glomerular cell cultures from rats with nephrotoxic serum nephritis. Clin Exp Immunol. 1989 Jan;75(1):123–128. [PMC free article] [PubMed] [Google Scholar]
- Sedor J. R., Nakazato Y., Konieczkowski M. Interleukin-1 and the mesangial cell. Kidney Int. 1992 Mar;41(3):595–599. doi: 10.1038/ki.1992.89. [DOI] [PubMed] [Google Scholar]
- Tipping P. G., Lowe M. G., Holdsworth S. R. Glomerular interleukin 1 production is dependent on macrophage infiltration in anti-GBM glomerulonephritis. Kidney Int. 1991 Jan;39(1):103–110. doi: 10.1038/ki.1991.13. [DOI] [PubMed] [Google Scholar]
- Tomosugi N. I., Cashman S. J., Hay H., Pusey C. D., Evans D. J., Shaw A., Rees A. J. Modulation of antibody-mediated glomerular injury in vivo by bacterial lipopolysaccharide, tumor necrosis factor, and IL-1. J Immunol. 1989 May 1;142(9):3083–3090. [PubMed] [Google Scholar]
- Werber H. I., Emancipator S. N., Tykocinski M. L., Sedor J. R. The interleukin 1 gene is expressed by rat glomerular mesangial cells and is augmented in immune complex glomerulonephritis. J Immunol. 1987 May 15;138(10):3207–3212. [PubMed] [Google Scholar]