Abstract
Flow cytometry showed that the enzyme lactate dehydrogenase (LDH-5) binds to peritoneal macrophages. The degree of binding appears to be genetically controlled and varies depending on the inbred strain of mouse. The rate at which LDH-5 is cleared from the circulation correlates with the degree of binding to macrophages. These findings suggest that the binding of LDH-5 to macrophages may serve as an in-vitro index of enzyme clearance.
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Selected References
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- Hayashi T., Ozaki M., Mori I., Saito M., Itoh T., Yamamoto H. Enhanced clearance of lactic dehydrogenase-5 in severe combined immunodeficiency (SCID) mice: effect of lactic dehydrogenase virus on enzyme clearance. Int J Exp Pathol. 1992 Apr;73(2):173–181. [PMC free article] [PubMed] [Google Scholar]
- Hayashi T., Salata K., Kingman A., Notkins A. L. Regulation of enzyme levels in the blood. Influence of environmental and genetic factors on enzyme clearance. Am J Pathol. 1988 Sep;132(3):503–511. [PMC free article] [PubMed] [Google Scholar]
- Mahy B. W., Rowson K. E., Parr C. W. Studies on the mechanism of action of Riley virus. IV. The reticuloendothelial system and impaired plasma enzyme clearance in infected mice. J Exp Med. 1967 Feb 1;125(2):277–288. doi: 10.1084/jem.125.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NOTKINS A. L. LACTIC DEHYDROGENASE VIRUS. Bacteriol Rev. 1965 Jun;29:143–160. doi: 10.1128/br.29.2.143-160.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NOTKINS A. L., SCHEELE C. IMPAIRED CLEARANCE OF ENZYMES IN MICE INFECTED WITH THE LACTIC DEHYDROGENASE AGENT. J Natl Cancer Inst. 1964 Oct;33:741–749. [PubMed] [Google Scholar]
- Nakayama H., Hayashi T., Salata K. F., Notkins A. L. Flow cytometry to identify cell types to which enzymes bind. Effect of lactic dehydrogenase virus on enzyme binding. J Biol Chem. 1990 Aug 25;265(24):14355–14357. [PubMed] [Google Scholar]
- Notkins A. L. Enzymatic and immunologic alterations in mice infected with lactic dehydrogenase virus. Am J Pathol. 1971 Sep;64(3):733–746. [PMC free article] [PubMed] [Google Scholar]
- Sinke J., Bouma J. M., Kooistra T., Gruber M. Endocytosis and breakdown of 125I-labelled lactate dehydrogenase isoenzyme M4 by rat liver and spleen in vivo. Biochem J. 1979 Apr 15;180(1):1–9. doi: 10.1042/bj1800001. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smit M. J., Duursma A. M., Bouma J. M., Gruber M. Receptor-mediated endocytosis of lactate dehydrogenase M4 by liver macrophages: a mechanism for elimination of enzymes from plasma. Evidence for competition by creatine kinase MM, adenylate kinase, malate, and alcohol dehydrogenase. J Biol Chem. 1987 Sep 25;262(27):13020–13026. [PubMed] [Google Scholar]