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. 1987 Oct;56(4):383–388. doi: 10.1038/bjc.1987.211

Stromal sensitivity to radiation and hyperthermia.

S A Hill 1, K A Smith 1, J Denekamp 1
PMCID: PMC2001813  PMID: 2446644

Abstract

The influence on stroma of heat alone, X-rays alone or the combined treatment, has been studied using the tumour bed effect (TBE) as an assay. Ca NT cells have been implanted into previously treated subcutaneous sites as an angiogenic stimulus. The vascular damage is then assessed by the reduced tumour growth rate, which results from inadequate vascular proliferation. A range of X-ray doses was used and large alterations in latent period for growth to 2 mm diameter were followed by smaller alterations in the growth rate of established tumours. A dose response relationship was seen for latency (0-20 Gy) and for growth rate (0-16 Gy). A range of subcutaneous temperatures was obtained by immersion in a water bath for 60 minutes at 40 degrees, 41.5 degrees, 43 degrees or 44.5 degrees C. A slight retardation of tumour growth was seen after 41.5 degrees C, but an unexpected acceleration resulted from the highest heat treatment. Combined heat and X-ray treatments showed thermal sensitization of the X-ray induced TBE at 41.5 degrees C, with a reversal at higher temperatures. At 43 degrees C and 44.5 degrees C a mild thermal burn was induced and this appeared to elicit neovascularisation that could be utilized by the implanted tumour cells. Delayed implantation of tumour cells (at 4 weeks instead of 1 day) abolished this effect.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barendsen G. W., Broerse J. J. Experimental radiotherapy of a rat rhabdomyosarcoma with 15 MeV neutrons and 300 kV x-rays. I. Effects of single exposures. Eur J Cancer. 1969 Sep;5(4):373–391. doi: 10.1016/0014-2964(69)90051-6. [DOI] [PubMed] [Google Scholar]
  2. Clifton K. H., Jirtle R. Mammary carcinoma cell population growth in preirradiated and unirradiated transplant sites. Viable tumor growth, vascularity, and the tumor-bed effect. Radiology. 1975 Nov;117(2):459–465. doi: 10.1148/117.2.459. [DOI] [PubMed] [Google Scholar]
  3. Denekamp J. Cell kinetics and radiation biology. Int J Radiat Biol Relat Stud Phys Chem Med. 1986 Feb;49(2):357–380. doi: 10.1080/09553008514552591. [DOI] [PubMed] [Google Scholar]
  4. Dewhirst M. W., Sim D. A., Wilson S., DeYoung D., Parsells J. L. Correlation between initial and long-term responses of spontaneous pet animal tumors to heat and radiation or radiation alone. Cancer Res. 1983 Dec;43(12 Pt 1):5735–5741. [PubMed] [Google Scholar]
  5. Dudar T. E., Jain R. K. Differential response of normal and tumor microcirculation to hyperthermia. Cancer Res. 1984 Feb;44(2):605–612. [PubMed] [Google Scholar]
  6. Emami B., Song C. W. Physiological mechanisms in hyperthermia: a review. Int J Radiat Oncol Biol Phys. 1984 Feb;10(2):289–295. doi: 10.1016/0360-3016(84)90015-4. [DOI] [PubMed] [Google Scholar]
  7. Fajardo L. F., Egbert B., Marmor J., Hahn G. M. Effects of hyperthermia in a malignant tumor. Cancer. 1980 Feb;45(3):613–623. doi: 10.1002/1097-0142(19800201)45:3<613::aid-cncr2820450331>3.0.co;2-e. [DOI] [PubMed] [Google Scholar]
  8. Fajardo L. F., Schreiber A. B., Kelly N. I., Hahn G. M. Thermal sensitivity of endothelial cells. Radiat Res. 1985 Aug;103(2):276–285. [PubMed] [Google Scholar]
  9. Hewitt H. B., Blake E. R. The growth of transplanted murine tumours in pre-irradiated sites. Br J Cancer. 1968 Dec;22(4):808–824. doi: 10.1038/bjc.1968.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hobson B., Denekamp J. Endothelial proliferation in tumours and normal tissues: continuous labelling studies. Br J Cancer. 1984 Apr;49(4):405–413. doi: 10.1038/bjc.1984.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ito H., Barkley T., Jr, Peters L. J., Milas L. Modification of tumor response to cyclophosphamide and irradiation by preirradiation of the tumor bed: prolonged growth delay but reduced curability. Int J Radiat Oncol Biol Phys. 1985 Mar;11(3):547–553. doi: 10.1016/0360-3016(85)90186-5. [DOI] [PubMed] [Google Scholar]
  12. Law M. P., Ahier R. G., Field S. B. The response of the mouse ear to heat applied alone or combined with X rays. Br J Radiol. 1978 Feb;51(602):132–138. doi: 10.1259/0007-1285-51-602-132. [DOI] [PubMed] [Google Scholar]
  13. Marmor J. B., Hilerio F. J., Hahn G. M. Tumor eradication and cell survival after localized hyperthermia induced by ultrasound. Cancer Res. 1979 Jun;39(6 Pt 1):2166–2171. [PubMed] [Google Scholar]
  14. Morris C. C., Myers R., Field S. B. The response of the rat tail to hyperthermia. Br J Radiol. 1977 Aug;50(596):576–580. doi: 10.1259/0007-1285-50-596-576. [DOI] [PubMed] [Google Scholar]
  15. Reinhold H. S., Endrich B. Tumour microcirculation as a target for hyperthermia. Int J Hyperthermia. 1986 Apr-Jun;2(2):111–137. doi: 10.3109/02656738609012389. [DOI] [PubMed] [Google Scholar]
  16. Rofstad E. K., Brustad T. Primary and secondary cell death in human melanoma xenografts following hyperthermic treatment. Cancer Res. 1986 Jan;46(1):355–361. [PubMed] [Google Scholar]
  17. STENSTROM K. W., VERMUND H., MOSSER D. G., MARVIN J. F. Effects of roentgen irradiation on the tumor bed. I. The inhibiting action of local pretransplantation roentgen irradiation (1500 r alpha) on the growth of mouse mammary carcinoma. Radiat Res. 1955 Apr;2(2):180–191. [PubMed] [Google Scholar]
  18. Sheldon P. W., Hill S. A. Hypoxic cell radiosensitizers and local control by X-ray of a transplanted tumour in mice. Br J Cancer. 1977 Jun;35(6):795–808. doi: 10.1038/bjc.1977.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Song C. W., Kang M. S., Rhee J. G., Levitt S. H. Vascular damage and delayed cell death in tumours after hyperthermia. Br J Cancer. 1980 Feb;41(2):309–312. doi: 10.1038/bjc.1980.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Thomlinson R. H., Craddock E. A. The gross response of an experimental tumour to single doses of x-rays. Br J Cancer. 1967 Mar;21(1):108–123. doi: 10.1038/bjc.1967.10. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Urano M., Cunningham M. Insignificant tumor bed effect after pretransplantation hyperthermia. Cancer Res. 1980 Jan;40(1):26–28. [PubMed] [Google Scholar]
  22. Van Den Brenk H. A., Stone M., Kelly H., Orton C., Sharpington C. Promotion of growth of tumour cells in acutely inflamed tissues. Br J Cancer. 1974 Sep;30(3):246–260. doi: 10.1038/bjc.1974.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Walker A., Wheldon T. E., Brunton G. F., Abdelaal A. S. Contrasting regrowth delay responses of a murine tumour to isocurative hyperthermia or X irradiation. Br J Radiol. 1982 Oct;55(658):780–782. doi: 10.1259/0007-1285-55-658-780. [DOI] [PubMed] [Google Scholar]
  24. Wheldon T. E., Hingston E. C. Differential effect of hyperthermia and x-irradiation on regrowth rate and tumour-bed effect for a rat sarcoma. Br J Cancer. 1982 Feb;45(2):265–271. doi: 10.1038/bjc.1982.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wondergem J., Begg A. C., Haveman J. Effects of hyperthermia and X-irradiation on mouse stromal tissue. Int J Radiat Biol Relat Stud Phys Chem Med. 1986 Jul;50(1):65–76. doi: 10.1080/09553008614550451. [DOI] [PubMed] [Google Scholar]

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