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International Journal of Experimental Pathology logoLink to International Journal of Experimental Pathology
. 1991 Aug;72(4):397–405.

Immunocytochemical investigation of the tissue of origin of two lung cancer cell lines.

M R Daniel 1, H E Burnett 1
PMCID: PMC2001968  PMID: 1883740

Abstract

The constitutive cell types of the two lung cancer cell lines CaLu1 and CaLu3 have been investigated by immunocytochemical staining with markers for cytokeratins, vimentin, carcinoembryonic antigen and the epithelial cell epitope recognized by the monoclonal antibody Ber-EP4. The cells of both lines reacted with vimentin, with CAM 5.2, which is a marker for cytokeratins 8, 18 and 19, and with a specific marker for cytokeratin 19. CaLu3 cultures showed patchy staining, and CaLu1 none, when treated with a monoclonal antibody reactive with cytokeratins 13, 14 and 17. CaLu3 cells all reacted strongly with Ber-EP4 and the antibody to carcinoembryonic antigen, whereas CaLu1 gave a negative reaction with both these reagents. These results indicate that the CaLu3 line has retained antigens characteristic of some bronchial adenocarcinomata, and that the CaLu1 line was derived from a mesothelioma rather than a pleural metastasis of a squamous carcinoma.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartek J., Durban E. M., Hallowes R. C., Taylor-Papadimitriou J. A subclass of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins. J Cell Sci. 1985 Apr;75:17–33. doi: 10.1242/jcs.75.1.17. [DOI] [PubMed] [Google Scholar]
  2. Blobel G. A., Moll R., Franke W. W., Kayser K. W., Gould V. E. The intermediate filament cytoskeleton of malignant mesotheliomas and its diagnostic significance. Am J Pathol. 1985 Nov;121(2):235–247. [PMC free article] [PubMed] [Google Scholar]
  3. Blobel G. A., Moll R., Franke W. W., Vogt-Moykopf I. Cytokeratins in normal lung and lung carcinomas. I. Adenocarcinomas, squamous cell carcinomas and cultured cell lines. Virchows Arch B Cell Pathol Incl Mol Pathol. 1984;45(4):407–429. doi: 10.1007/BF02889883. [DOI] [PubMed] [Google Scholar]
  4. Broers J. L., Ramaekers F. C., Rot M. K., Oostendorp T., Huysmans A., van Muijen G. N., Wagenaar S. S., Vooijs G. P. Cytokeratins in different types of human lung cancer as monitored by chain-specific monoclonal antibodies. Cancer Res. 1988 Jun 1;48(11):3221–3229. [PubMed] [Google Scholar]
  5. Castor C. W., Naylor B. Characteristics of normal and malignant human mesothelial cells studied in vitro. Lab Invest. 1969 May;20(5):437–443. [PubMed] [Google Scholar]
  6. Gatter K. C., Dunnill M. S., Pulford K. A., Heryet A., Mason D. Y. Human lung tumours: a correlation of antigenic profile with histological type. Histopathology. 1985 Aug;9(8):805–823. doi: 10.1111/j.1365-2559.1985.tb02868.x. [DOI] [PubMed] [Google Scholar]
  7. Gatter K. C., Dunnill M. S., Van Muijen G. N., Mason D. Y. Human lung tumours may coexpress different classes of intermediate filaments. J Clin Pathol. 1986 Sep;39(9):950–954. doi: 10.1136/jcp.39.9.950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ghosh A. K., Gatter K. C., Dunnill M. S., Mason D. Y. Immunohistological staining of reactive mesothelium, mesothelioma, and lung carcinoma with a panel of monoclonal antibodies. J Clin Pathol. 1987 Jan;40(1):19–25. doi: 10.1136/jcp.40.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Golombick T., Dansey R., Bezwoda W. R., Rosendorff J. Establishment and characterization of two new human ovarian cancer cell lines UWOV1 and UWOV2 and a subline UWOV2 (Sf) growing in serum-free conditions: growth characteristics, biochemical, and cytogenetic studies. In Vitro Cell Dev Biol. 1990 May;26(5):447–454. doi: 10.1007/BF02624086. [DOI] [PubMed] [Google Scholar]
  10. Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
  11. Latza U., Niedobitek G., Schwarting R., Nekarda H., Stein H. Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelial. J Clin Pathol. 1990 Mar;43(3):213–219. doi: 10.1136/jcp.43.3.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Leighton J., Estes L. W., Mansukhani S., Brada Z. A cell line derived from normal dog kidney (MDCK) exhibiting qualities of papillary adenocarcinoma and of renal tubular epithelium. Cancer. 1970 Nov;26(5):1022–1028. doi: 10.1002/1097-0142(197011)26:5<1022::aid-cncr2820260509>3.0.co;2-m. [DOI] [PubMed] [Google Scholar]
  13. Okamura A., Ohkawa J., Fujisawa H., Sashikata T. Clinicopathological study on the relationship between serum-CEA and tissue-CEA of resected lung cancer cases. Acta Pathol Jpn. 1984 Nov;34(6):1209–1219. doi: 10.1111/j.1440-1827.1984.tb00549.x. [DOI] [PubMed] [Google Scholar]
  14. Osborn M., Debus E., Weber K. Monoclonal antibodies specific for vimentin. Eur J Cell Biol. 1984 May;34(1):137–143. [PubMed] [Google Scholar]
  15. Shimada T., Nakamura F. Dome formation of keratin-containing agranular cells from rat anterior pituitary gland in vitro. In Vitro Cell Dev Biol. 1988 Dec;24(12):1193–1200. doi: 10.1007/BF02624189. [DOI] [PubMed] [Google Scholar]
  16. Virtanen I., Lehto V. P., Lehtonen E., Vartio T., Stenman S., Kurki P., Wager O., Small J. V., Dahl D., Badley R. A. Expression of intermediate filaments in cultured cells. J Cell Sci. 1981 Aug;50:45–63. doi: 10.1242/jcs.50.1.45. [DOI] [PubMed] [Google Scholar]

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