Skip to main content
NCBI home page
International Journal of Experimental Pathology logoLink to International Journal of Experimental Pathology
. 1992 Apr;73(2):231–239.

Comparative studies on inflammatory reactions induced by non-immunological and immunological stimuli in an air pouch and in a carboxymethyl cellulose (CMC)-induced inflammatory pouch.

M Isaji 1, J Naito 1
PMCID: PMC2001992  PMID: 1571282

Abstract

Inflammatory reactions were compared in the air pouch and the CMC pouch. Inflammation was induced by injection of CMC as a non-specific irritant, lipopolysaccharide (LPS) as an activator of macrophages, and methylated bovine serum albumin (m-BSA) as an inducer of delayed type hypersensitivity. There was no prominent difference in the inflammatory reactions following injection of 2% CMC solution into a 4-day-old air pouch and a 3-day-old CMC pouch. On the other hand, injection of 4 ml of 100 ng/ml LPS into each of the pouches enhanced the inflammatory reactions in the CMC pouch several-fold compared with those in the air pouch. A similar tendency was found in the case of an injection of 1 ml of 1 mg/ml m-BSA in rats sensitized with 1 mg of m-BSA. The enhanced inflammatory reactions induced by the injection of LPS or m-BSA were inhibited by dexamethasone, but not by indomethacin. These results indicate that the enhanced inflammatory reactions induced by CMC are related to lining tissue formation, which was a common characteristic in both pouches. Enhanced inflammatory reactions following injection of LPS and m-BSA were related to the activation of macrophages and newly formed blood vessels, which were not characteristic features in the air pouch, but were in the CMC pouch, in addition to the lining tissue. Cyclo-oxygenase products were not associated with the reactions.

Full text

PDF
231

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cater D. B., Wallington T. B. Inflammatory changes in newly formed vessels of carrageenin-induced granulomas after systemic 5-hydroxytryptamine, bradykinin, kallikrein, or lysolecithin. Br J Exp Pathol. 1968 Feb;49(1):74–80. [PMC free article] [PubMed] [Google Scholar]
  2. Chensue S. W., Otterness I. G., Higashi G. I., Forsch C. S., Kunkel S. L. Monokine production by hypersensitivity (Schistosoma mansoni egg) and foreign body (Sephadex bead)-type granuloma macrophages. Evidence for sequential production of IL-1 and tumor necrosis factor. J Immunol. 1989 Feb 15;142(4):1281–1286. [PubMed] [Google Scholar]
  3. Dinarello C. A., Endres S. Role for interleukin-1 in the pathogenesis of hypersensitivity diseases. J Cell Biochem. 1989 Mar;39(3):229–238. doi: 10.1002/jcb.240390303. [DOI] [PubMed] [Google Scholar]
  4. Hambleton P., Miller P. Studies on immunological air pouch inflammation in the rat. Int Arch Allergy Appl Immunol. 1988;87(1):70–75. doi: 10.1159/000234651. [DOI] [PubMed] [Google Scholar]
  5. Harmsen A. G. Role of alveolar macrophages in lipopolysaccharide-induced neutrophil accumulation. Infect Immun. 1988 Aug;56(8):1858–1863. doi: 10.1128/iai.56.8.1858-1863.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Isaji M., Momose Y., Naito J. Enhancement of inflammatory reactions in a non-immunological air pouch model in rats. Br J Exp Pathol. 1989 Dec;70(6):705–716. [PMC free article] [PubMed] [Google Scholar]
  7. Issekutz A. C., Megyeri P., Issekutz T. B. Role for macrophage products in endotoxin-induced polymorphonuclear leukocyte accumulation during inflammation. Lab Invest. 1987 Jan;56(1):49–59. [PubMed] [Google Scholar]
  8. Jirik F. R., Podor T. J., Hirano T., Kishimoto T., Loskutoff D. J., Carson D. A., Lotz M. Bacterial lipopolysaccharide and inflammatory mediators augment IL-6 secretion by human endothelial cells. J Immunol. 1989 Jan 1;142(1):144–147. [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Larrick J. W., Kunkel S. L. The role of tumor necrosis factor and interleukin 1 in the immunoinflammatory response. Pharm Res. 1988 Mar;5(3):129–139. doi: 10.1023/a:1015904721223. [DOI] [PubMed] [Google Scholar]
  11. Nosál R., Drábiková K., Pecivová J., Ondrias K. Membrane perturbing activity of beta-adrenoceptor blocking drugs in isolated rat mast cells. Agents Actions. 1989 Apr;27(1-2):36–38. doi: 10.1007/BF02222191. [DOI] [PubMed] [Google Scholar]
  12. Sedgwick A. D., Sin Y. M., Edwards J. C., Willoughby D. A. Increased inflammatory reactivity in newly formed lining tissue. J Pathol. 1983 Dec;141(4):483–495. doi: 10.1002/path.1711410406. [DOI] [PubMed] [Google Scholar]

Articles from International Journal of Experimental Pathology are provided here courtesy of Wiley

RESOURCES