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International Journal of Experimental Pathology logoLink to International Journal of Experimental Pathology
. 1993 Jun;74(3):243–250.

Asbestos fibre length-dependent detachment injury to alveolar epithelial cells in vitro: role of a fibronectin-binding receptor.

K Donaldson 1, B G Miller 1, E Sara 1, J Slight 1, R C Brown 1
PMCID: PMC2002156  PMID: 8392859

Abstract

A short and a long fibre sample of amosite asbestos were tested for their effects on cells of the human Type 2 alveolar epithelial cell-line A549 in vitro. The long amosite sample was found to cause a rapid detachment of the epithelial cells live from their substratum. At the highest dose, on average 28% of the cells present were detached in this way. Studies on the mechanism of the detachment injury showed that it did not involve oxidants since it was not ameliorated by scavengers of active oxygen species. Neither was the effect reduced by treatment of the fibres with the iron chelator Desferal. Treatments reported to increase the interaction between fibres and cells, serum and poly-L-lysine, did not influence the detachment injury, nor did lung lining fluid. Conversely, the fibronectin tripeptide RGD alone could cause detachment which suggested that a fibronectin-binding integrin was involved. This receptor could be reduced in activity by long fibre exposure, leading to detachment. The detaching effect of fibre could be mimicked by the protein kinase C activator PMA, and so the second messenger system of the cell could also be involved. This type of injury could be important in the pathology associated with exposure to long fibres.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adamson I. Y., Bowden D. H. Response of mouse lung to crocidolite asbestos. 1. Minimal fibrotic reaction to short fibres. J Pathol. 1987 Jun;152(2):99–107. doi: 10.1002/path.1711520206. [DOI] [PubMed] [Google Scholar]
  2. Adamson I. Y., Hedgecock C., Bowden D. H. Epithelial cell-fibroblast interactions in lung injury and repair. Am J Pathol. 1990 Aug;137(2):385–392. [PMC free article] [PubMed] [Google Scholar]
  3. Adamson I. Y., Young L., Bowden D. H. Relationship of alveolar epithelial injury and repair to the induction of pulmonary fibrosis. Am J Pathol. 1988 Feb;130(2):377–383. [PMC free article] [PubMed] [Google Scholar]
  4. Albelda S. M. Endothelial and epithelial cell adhesion molecules. Am J Respir Cell Mol Biol. 1991 Mar;4(3):195–203. doi: 10.1165/ajrcmb/4.3.195. [DOI] [PubMed] [Google Scholar]
  5. Brody A. R., Overby L. H. Incorporation of tritiated thymidine by epithelial and interstitial cells in bronchiolar-alveolar regions of asbestos-exposed rats. Am J Pathol. 1989 Jan;134(1):133–140. [PMC free article] [PubMed] [Google Scholar]
  6. Brody A. R., Roe M. W., Evans J. N., Davis G. S. Deposition and translocation of inhaled silica in rats. Quantification of particle distribution, macrophage participation, and function. Lab Invest. 1982 Dec;47(6):533–542. [PubMed] [Google Scholar]
  7. Brody A. R., Soler P., Basset F., Haschek W. M., Witschi H. Epithelial-mesenchymal associations of cells in human pulmonary fibrosis and in BHT-oxygen-induced fibrosis in mice. Exp Lung Res. 1981 Aug;2(3):207–220. doi: 10.3109/01902148109052316. [DOI] [PubMed] [Google Scholar]
  8. Brown G. M., Cowie H., Davis J. M., Donaldson K. In vitro assays for detecting carcinogenic mineral fibres: a comparison of two assays and the role of fibre size. Carcinogenesis. 1986 Dec;7(12):1971–1974. doi: 10.1093/carcin/7.12.1971. [DOI] [PubMed] [Google Scholar]
  9. Brown R. C., Carthew P., Hoskins J. A., Sara E., Simpson C. F. Surface modification can affect the carcinogenicity of asbestos. Carcinogenesis. 1990 Oct;11(10):1883–1885. doi: 10.1093/carcin/11.10.1883. [DOI] [PubMed] [Google Scholar]
  10. Brown R. C., Sara E. A., Hoskins J. A., Evans C. E. Factors affecting the interaction of asbestos fibres with mammalian cells: a study using cells in suspension. Ann Occup Hyg. 1991 Feb;35(1):25–34. doi: 10.1093/annhyg/35.1.25. [DOI] [PubMed] [Google Scholar]
  11. Davis J. M., Addison J., Bolton R. E., Donaldson K., Jones A. D., Smith T. The pathogenicity of long versus short fibre samples of amosite asbestos administered to rats by inhalation and intraperitoneal injection. Br J Exp Pathol. 1986 Jun;67(3):415–430. [PMC free article] [PubMed] [Google Scholar]
  12. Donaldson K., Brown G. M., Brown D. M., Bolton R. E., Davis J. M. Inflammation generating potential of long and short fibre amosite asbestos samples. Br J Ind Med. 1989 Apr;46(4):271–276. doi: 10.1136/oem.46.4.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Donaldson K., Slight J., Brown G. M., Bolton R. E. The ability of inflammatory bronchoalveolar leucocyte populations elicited with microbes or mineral dust to injure alveolar epithelial cells and degrade extracellular matrix in vitro. Br J Exp Pathol. 1988 Jun;69(3):327–338. [PMC free article] [PubMed] [Google Scholar]
  14. Gellert A. R., Perry D., Langford J. A., Riches P. G., Rudd R. M. Asbestosis. Bronchoalveolar lavage fluid proteins and their relationship to pulmonary epithelial permeability. Chest. 1985 Nov;88(5):730–735. doi: 10.1378/chest.88.5.730. [DOI] [PubMed] [Google Scholar]
  15. Goodglick L. A., Kane A. B. Cytotoxicity of long and short crocidolite asbestos fibers in vitro and in vivo. Cancer Res. 1990 Aug 15;50(16):5153–5163. [PubMed] [Google Scholar]
  16. Goodglick L. A., Kane A. B. Role of reactive oxygen metabolites in crocidolite asbestos toxicity to mouse macrophages. Cancer Res. 1986 Nov;46(11):5558–5566. [PubMed] [Google Scholar]
  17. Hesterberg T. W., Barrett J. C. Dependence of asbestos- and mineral dust-induced transformation of mammalian cells in culture on fiber dimension. Cancer Res. 1984 May;44(5):2170–2180. [PubMed] [Google Scholar]
  18. Hobson J., Wright J. L., Churg A. Active oxygen species mediate asbestos fiber uptake by tracheal epithelial cells. FASEB J. 1990 Oct;4(13):3135–3139. doi: 10.1096/fasebj.4.13.2170219. [DOI] [PubMed] [Google Scholar]
  19. Kamp D. W., Dunne M., Anderson J. A., Weitzman S. A., Dunn M. M. Serum promotes asbestos-induced injury to human pulmonary epithelial cells. J Lab Clin Med. 1990 Sep;116(3):289–297. [PubMed] [Google Scholar]
  20. Lieber M., Smith B., Szakal A., Nelson-Rees W., Todaro G. A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int J Cancer. 1976 Jan 15;17(1):62–70. doi: 10.1002/ijc.2910170110. [DOI] [PubMed] [Google Scholar]
  21. McGavran P. D., Brody A. R. Chrysotile asbestos inhalation induces tritiated thymidine incorporation by epithelial cells of distal bronchioles. Am J Respir Cell Mol Biol. 1989 Sep;1(3):231–235. doi: 10.1165/ajrcmb/1.3.231. [DOI] [PubMed] [Google Scholar]
  22. Moalli P. A., MacDonald J. L., Goodglick L. A., Kane A. B. Acute injury and regeneration of the mesothelium in response to asbestos fibers. Am J Pathol. 1987 Sep;128(3):426–445. [PMC free article] [PubMed] [Google Scholar]
  23. Mossman B. T., Kessler J. B., Ley B. W., Craighead J. E. Interaction of crocidolite asbestos with hamster respiratory mucosa in organ culture. Lab Invest. 1977 Feb;36(2):131–139. [PubMed] [Google Scholar]
  24. Mossman B. T., Marsh J. P. Evidence supporting a role for active oxygen species in asbestos-induced toxicity and lung disease. Environ Health Perspect. 1989 May;81:91–94. doi: 10.1289/ehp.898191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Osornio-Vargas A. R., Hernández-Rodríguez N. A., Yáez-Buruel A. G., Ussler W., Overby L. H., Brody A. R. Lung cell toxicity experimentally induced by a mixed dust from Mexicali, Baja California, Mexico. Environ Res. 1991 Oct;56(1):31–47. doi: 10.1016/s0013-9351(05)80107-0. [DOI] [PubMed] [Google Scholar]
  26. Rickard K. A., Shoji S., Spurzem J. R., Rennard S. I. Attachment characteristics of bovine bronchial epithelial cells to extracellular matrix components. Am J Respir Cell Mol Biol. 1991 May;4(5):440–448. doi: 10.1165/ajrcmb/4.5.440. [DOI] [PubMed] [Google Scholar]
  27. Rom W. N., Travis W. D., Brody A. R. Cellular and molecular basis of the asbestos-related diseases. Am Rev Respir Dis. 1991 Feb;143(2):408–422. doi: 10.1164/ajrccm/143.2.408. [DOI] [PubMed] [Google Scholar]
  28. Scheule R. K., Holian A. IgG specifically enhances chrysotile asbestos-stimulated superoxide anion production by the alveolar macrophage. Am J Respir Cell Mol Biol. 1989 Oct;1(4):313–318. doi: 10.1165/ajrcmb/1.4.313. [DOI] [PubMed] [Google Scholar]
  29. Shatos M. A., Doherty J. M., Marsh J. P., Mossman B. T. Prevention of asbestos-induced cell death in rat lung fibroblasts and alveolar macrophages by scavengers of active oxygen species. Environ Res. 1987 Oct;44(1):103–116. doi: 10.1016/s0013-9351(87)80090-7. [DOI] [PubMed] [Google Scholar]
  30. Warheit D. B., Chang L. Y., Hill L. H., Hook G. E., Crapo J. D., Brody A. R. Pulmonary macrophage accumulation and asbestos-induced lesions at sites of fiber deposition. Am Rev Respir Dis. 1984 Feb;129(2):301–310. [PubMed] [Google Scholar]
  31. Woodworth C. D., Mossman B. T., Craighead J. E. Comparative effects of fibrous and nonfibrous minerals on cells and liposomes. Environ Res. 1982 Feb;27(1):190–205. doi: 10.1016/0013-9351(82)90070-6. [DOI] [PubMed] [Google Scholar]

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