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International Journal of Experimental Pathology logoLink to International Journal of Experimental Pathology
. 1993 Jun;74(3):291–297.

Cytokine release from human peripheral blood leucocytes incubated with endotoxin with and without prior infection with influenza virus: relevance to the sudden infant death syndrome.

J B Lundemose 1, H Smith 1, C Sweet 1
PMCID: PMC2002163  PMID: 8392861

Abstract

Previous work with a neonatal ferret model for human SIDS had indicated that inflammation caused by a combination of influenza virus and bacterial endotoxin may be a cause of human SIDS. To determine whether cytokines may be involved in this inflammatory response, levels of interleukin (IL)-1 beta, IL-6 and tumour necrosis factor (TNF)-alpha were examined, using ELISA assays, in culture supernatants of human peripheral blood leucocytes infected with influenza virus and subsequently incubated with endotoxin. Levels of TNF-alpha were increased compared to cells incubated with virus or endotoxin alone. Levels of IL-1 beta were also increased whereas levels of IL-6 were generally not enhanced. Cytokines appeared within 1-2 h of stimulation with virus or endotoxin and increased subsequently to reach maximum titres between 16 and 20 h post treatment. While levels of cytokine were much lower when determined using bioassays rather than ELISA assays, the pattern of increased yields from cells incubated with virus and endotoxin compared with either alone was still evident. The possible importance of these observations for SIDS victims is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Collie M. H., Rushton D. I., Sweet C., Smith H. Studies of influenza virus infection in newborn ferrets. J Med Microbiol. 1980 Nov;13(4):561–571. doi: 10.1099/00222615-13-4-561. [DOI] [PubMed] [Google Scholar]
  2. Gong J. H., Sprenger H., Hinder F., Bender A., Schmidt A., Horch S., Nain M., Gemsa D. Influenza A virus infection of macrophages. Enhanced tumor necrosis factor-alpha (TNF-alpha) gene expression and lipopolysaccharide-triggered TNF-alpha release. J Immunol. 1991 Nov 15;147(10):3507–3513. [PubMed] [Google Scholar]
  3. Helle M., Boeije L., Aarden L. A. Functional discrimination between interleukin 6 and interleukin 1. Eur J Immunol. 1988 Oct;18(10):1535–1540. doi: 10.1002/eji.1830181010. [DOI] [PubMed] [Google Scholar]
  4. Hinder F., Schmidt A., Gong J. H., Bender A., Sprenger H., Nain M., Gemsa D. Influenza A virus infects macrophages and stimulates release of tumor necrosis factor-alpha. Pathobiology. 1991;59(4):227–231. doi: 10.1159/000163651. [DOI] [PubMed] [Google Scholar]
  5. Husseini R. H., Collie M. H., Rushton D. I., Sweet C., Smith H. The role of naturally-acquired bacterial infection in influenza-related death in neonatal ferrets. Br J Exp Pathol. 1983 Oct;64(5):559–569. [PMC free article] [PubMed] [Google Scholar]
  6. Jakeman K. J., Rushton D. I., Smith H., Sweet C. Exacerbation of bacterial toxicity to infant ferrets by influenza virus: possible role in sudden infant death syndrome. J Infect Dis. 1991 Jan;163(1):35–40. doi: 10.1093/infdis/163.1.35. [DOI] [PubMed] [Google Scholar]
  7. Lee S., Barson A. J., Drucker D. B., Morris J. A., Telford D. R. Lethal challenge of gnotobiotic weanling rats with bacterial isolates from cases of sudden infant death syndrome (SIDS). J Clin Pathol. 1987 Dec;40(12):1393–1396. doi: 10.1136/jcp.40.12.1393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lonnemann G., Endres S., Van der Meer J. W., Cannon J. G., Koch K. M., Dinarello C. A. Differences in the synthesis and kinetics of release of interleukin 1 alpha, interleukin 1 beta and tumor necrosis factor from human mononuclear cells. Eur J Immunol. 1989 Sep;19(9):1531–1536. doi: 10.1002/eji.1830190903. [DOI] [PubMed] [Google Scholar]
  9. Matsuyama T., Sweet C., Collie M. H., Smith H. Aspects of virulence in ferrets exhibited by influenza virus recombinants of known genetic constitution. J Infect Dis. 1980 Mar;141(3):351–361. doi: 10.1093/infdis/141.3.351. [DOI] [PubMed] [Google Scholar]
  10. Mizel S. B., Oppenheim J. J., Rosentreich D. L. Characterization of lymphocyte-activating factor (LAF) produced by a macrophage cell line, P388D1. II. Biochemical characterization of LAF induced by activated T cells and LPS. J Immunol. 1978 May;120(5):1504–1508. [PubMed] [Google Scholar]
  11. Morris J. A., Haran D., Smith A. Hypothesis: common bacterial toxins are a possible cause of the sudden infant death syndrome. Med Hypotheses. 1987 Feb;22(2):211–222. doi: 10.1016/0306-9877(87)90145-9. [DOI] [PubMed] [Google Scholar]
  12. Nain M., Hinder F., Gong J. H., Schmidt A., Bender A., Sprenger H., Gemsa D. Tumor necrosis factor-alpha production of influenza A virus-infected macrophages and potentiating effect of lipopolysaccharides. J Immunol. 1990 Sep 15;145(6):1921–1928. [PubMed] [Google Scholar]
  13. Newton R. C. Human monocyte production of interleukin-1: parameters of the induction of interleukin-1 secretion by lipopolysaccharides. J Leukoc Biol. 1986 Mar;39(3):299–311. doi: 10.1002/jlb.39.3.299. [DOI] [PubMed] [Google Scholar]
  14. Roberts N. J., Jr, Horan P. K. Expression of viral antigens after infection of human lymphocytes, monocytes, and macrophages with influenza virus. J Infect Dis. 1985 Feb;151(2):308–313. doi: 10.1093/infdis/151.2.308. [DOI] [PubMed] [Google Scholar]
  15. Rodgers B. C., Mims C. A. Influenza virus replication in human alveolar macrophages. J Med Virol. 1982;9(3):177–184. doi: 10.1002/jmv.1890090304. [DOI] [PubMed] [Google Scholar]
  16. Scott D. J., Gardner P. S., McQuillin J., Stanton A. N., Downham M. A. Respiratory viruses and cot death. Br Med J. 1978 Jul 1;2(6129):12–13. doi: 10.1136/bmj.2.6129.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sonnabend O. A., Sonnabend W. F., Krech U., Molz G., Sigrist T. Continuous microbiological and pathological study of 70 sudden and unexpected infant deaths: toxigenic intestinal clostridium botulinum infection in 9 cases of sudden infant death syndrome. Lancet. 1985 Feb 2;1(8423):237–241. doi: 10.1016/s0140-6736(85)91025-6. [DOI] [PubMed] [Google Scholar]
  18. Spofford B. T., Daynes R. A., Granger G. A. Cell-mediated immunity in vitro: a highly sensitive assay for human lymphotoxin. J Immunol. 1974 Jun;112(6):2111–2116. [PubMed] [Google Scholar]
  19. Sweet C., Bird R. A., Howie A. J., Overton H. A., Coates D. M., Smith H. Further studies of the reasons for the lack of alveolar infection during influenza in ferrets. Br J Exp Pathol. 1985 Apr;66(2):217–231. [PMC free article] [PubMed] [Google Scholar]
  20. Tapp E., Jones D. M., Tobin J. O. Interpretation of respiratory tract histology in cot deaths. J Clin Pathol. 1975 Nov;28(11):899–904. doi: 10.1136/jcp.28.11.899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tinsley C. M., Coates D. M., Sweet C., Smith H. Differential production of endogenous pyrogen by human peripheral blood leucocytes following interaction with H3N2 or H1N1 influenza viruses of differing virulence. Microb Pathog. 1987 Jul;3(1):63–70. doi: 10.1016/0882-4010(87)90038-6. [DOI] [PubMed] [Google Scholar]
  22. Vacheron F., Rudent A., Perin S., Labarre C., Quero A. M., Guenounou M. Production of interleukin 1 and tumour necrosis factor activities in bronchoalveolar washings following infection of mice by influenza virus. J Gen Virol. 1990 Feb;71(Pt 2):477–479. doi: 10.1099/0022-1317-71-2-477. [DOI] [PubMed] [Google Scholar]
  23. Valdes-Dapena M. A. Sudden and unexpected death in infancy: a review of the world literature 1954-1966. Pediatrics. 1967 Jan;39(1):123–138. [PubMed] [Google Scholar]
  24. Zink P., Drescher J., Verhagen W., Flik J., Milbradt H. Serological evidence of recent influenza virus A (H 3 N 2) infections in forensic cases of the sudden infant death syndrome (SIDS). Arch Virol. 1987;93(3-4):223–232. doi: 10.1007/BF01310976. [DOI] [PubMed] [Google Scholar]

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