Skip to main content
NCBI home page
International Journal of Experimental Pathology logoLink to International Journal of Experimental Pathology
. 1993 Jun;74(3):299–307.

Aluminium inhalation in sheep silicosis.

R Bégin 1, A Cantin 1, S Massé 1, A Dufresne 1, G Perreault 1, P Sébastien 1
PMCID: PMC2002168  PMID: 8334079

Abstract

In prior studies, we and others have documented a significant reduction by surface chemistry modification in the biological activity of quartz. We further documented that aluminium lactate inhalation one month after quartz exposure significantly suppressed the silica-induced alveolitis, reduced the pathological process and decreased the retention of quartz in the lung tissue. In the present study, we evaluated the efficacy of aluminium inhalation in altering the silicosis process after disease was recognized by standard chest radiography. Twenty-four sheep were enrolled in the study. The 14 silica exposed sheep had an abnormal chest radiograph of the ILO category 1 or above after 3 years of 100 mg Minusil-5 in 100-ml saline intratracheal injections. Ten control sheep were exposed to saline intratracheal injections. All sheep were evaluated at 3-month intervals by chest radiography, lung function and lung lavage. At month 36 of the study, all 14 sheep had an abnormal chest radiograph while the radiographs of controls remained normal. The sheep with silicosis had significantly reduced lung functions and increased cellularity, phospholipids and hyaluronan. These changes persisted during the next 12 months without exposure or treatment. At month 48 and at monthly intervals after, for 12 months, aerosol inhalations were carried out with saline alone for control and seven silicotic sheep and with 100 mg of aluminium lactate in 10 ml saline generated with a Bird Mark 8 pressure ventilator for the other seven silicotic sheep. All sheep were evaluated at 3-month intervals by chest radiography, lung function and lung lavage.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
299

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  2. Brody A. R., Roe M. W., Evans J. N., Davis G. S. Deposition and translocation of inhaled silica in rats. Quantification of particle distribution, macrophage participation, and function. Lab Invest. 1982 Dec;47(6):533–542. [PubMed] [Google Scholar]
  3. Bégin R. O., Cantin A. M., Boileau R. D., Bisson G. Y. Spectrum of alveolitis in quartz-exposed human subjects. Chest. 1987 Dec;92(6):1061–1067. doi: 10.1378/chest.92.6.1061. [DOI] [PubMed] [Google Scholar]
  4. Bégin R., Cantin A., Massé S. Recent advances in the pathogenesis and clinical assessment of mineral dust pneumoconioses: asbestosis, silicosis and coal pneumoconiosis. Eur Respir J. 1989 Nov;2(10):988–1001. [PubMed] [Google Scholar]
  5. Bégin R., Dufresne A., Cantin A., Possmayer F., Sébastien P., Fabi D., Bilodeau G., Martel M., Bisson D., Pietrowski B. Quartz exposure, retention, and early silicosis in sheep. Exp Lung Res. 1989 May;15(3):409–428. doi: 10.3109/01902148909087868. [DOI] [PubMed] [Google Scholar]
  6. Bégin R., Massé S., Sébastien P., Martel M., Bossé J., Dubois F., Geoffroy M., Labbé J. Sustained efficacy of aluminum to reduce quartz toxicity in the lung. Exp Lung Res. 1987;13(2):205–222. doi: 10.3109/01902148709064319. [DOI] [PubMed] [Google Scholar]
  7. Bégin R., Massé S., Sébastien P., Martel M., Geoffroy M., Labbé J. Late aluminum therapy reduces the cellular activities of simple silicosis in the sheep model. J Leukoc Biol. 1987 May;41(5):400–406. doi: 10.1002/jlb.41.5.400. [DOI] [PubMed] [Google Scholar]
  8. Bégin R., Possmayer F., Ormseth M. A., Martel M., Cantin A., Massé S. Effect of aluminum inhalation on alveolar phospholipid profiles in experimental silicosis. Lung. 1989;167(2):107–115. doi: 10.1007/BF02714937. [DOI] [PubMed] [Google Scholar]
  9. Cantin A., Allard C., Bégin R. Increased alveolar plasminogen activator in early asbestosis. Am Rev Respir Dis. 1989 Mar;139(3):604–609. doi: 10.1164/ajrccm/139.3.604. [DOI] [PubMed] [Google Scholar]
  10. Cantin A., Dubois F., Bégin R. Lung exposure to mineral dusts enhances the capacity of lung inflammatory cells to release superoxide. J Leukoc Biol. 1988 Apr;43(4):299–303. doi: 10.1002/jlb.43.4.299. [DOI] [PubMed] [Google Scholar]
  11. Davis G. S. Pathogenesis of silicosis: current concepts and hypotheses. Lung. 1986;164(3):139–154. doi: 10.1007/BF02713638. [DOI] [PubMed] [Google Scholar]
  12. Dubois F., Bégin R., Cantin A., Massé S., Martel M., Bilodeau G., Dufresne A., Perreault G., Sébastien P. Aluminum inhalation reduces silicosis in a sheep model. Am Rev Respir Dis. 1988 May;137(5):1172–1179. doi: 10.1164/ajrccm/137.5.1172. [DOI] [PubMed] [Google Scholar]
  13. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  14. Finkelstein M., Kusiak R., Suranyi G. Mortality among miners receiving workmen's compensation for silicosis in Ontario: 1940-1975. J Occup Med. 1982 Sep;24(9):663–667. doi: 10.1097/00043764-198209000-00012. [DOI] [PubMed] [Google Scholar]
  15. Gold E. W. A simple spectrophotometric method for estimating glycosaminoglycan concentrations. Anal Biochem. 1979 Oct 15;99(1):183–188. doi: 10.1016/0003-2697(79)90061-7. [DOI] [PubMed] [Google Scholar]
  16. Gordon S., Cohn Z. A. Bacille Calmette-Guérin infection in the mouse. Regulation of macrophage plasminogen activator by T lymphocytes and specific antigen. J Exp Med. 1978 Apr 1;147(4):1175–1188. doi: 10.1084/jem.147.4.1175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Killingsworth L. M., Savory J. Manual nephelometric methods for immunochemical determination of immunoglobulins IgG, IgA, and IgM in human serum. Clin Chem. 1972 Apr;18(4):335–339. [PubMed] [Google Scholar]
  18. Laurent U. B., Tengblad A. Determination of hyaluronate in biological samples by a specific radioassay technique. Anal Biochem. 1980 Dec;109(2):386–394. doi: 10.1016/0003-2697(80)90665-x. [DOI] [PubMed] [Google Scholar]
  19. Marchalonis J. J. An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochem J. 1969 Jun;113(2):299–305. doi: 10.1042/bj1130299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pick E., Keisari Y. Superoxide anion and hydrogen peroxide production by chemically elicited peritoneal macrophages--induction by multiple nonphagocytic stimuli. Cell Immunol. 1981 Apr;59(2):301–318. doi: 10.1016/0008-8749(81)90411-1. [DOI] [PubMed] [Google Scholar]
  21. Rouser G., Fkeischer S., Yamamoto A. Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids. 1970 May;5(5):494–496. doi: 10.1007/BF02531316. [DOI] [PubMed] [Google Scholar]
  22. Tengblad A. Affinity chromatography on immobilized hyaluronate and its application to the isolation of hyaluronate binding properties from cartilage. Biochim Biophys Acta. 1979 Jun 19;578(2):281–289. doi: 10.1016/0005-2795(79)90158-2. [DOI] [PubMed] [Google Scholar]
  23. ULMER D. D., VALLEE B. L., WACKER W. E. Metalloenzymes and myocardial infarction. II. Malic and lactic dehydrogenase activities and zinc concentrations in serum. N Engl J Med. 1956 Sep 6;255(10):450–456. doi: 10.1056/NEJM195609062551001. [DOI] [PubMed] [Google Scholar]
  24. Westerholm P. Silicosis. Observations on a case register. Scand J Work Environ Health. 1980;6 (Suppl 2):1–86. [PubMed] [Google Scholar]
  25. Ziskind M., Jones R. N., Weill H. Silicosis. Am Rev Respir Dis. 1976 May;113(5):643–665. doi: 10.1164/arrd.1976.113.5.643. [DOI] [PubMed] [Google Scholar]

Articles from International Journal of Experimental Pathology are provided here courtesy of Wiley

RESOURCES