Abstract
We have succeeded in inducing progressive splenomegaly by inoculating 9L-glioma cells into the back subcutaneous regions of Fischer rats. The progressive splenomegaly was histologically characterized by progressively increased extramedullary haematopoiesis. Immunohistochemically Leu 7 positive cells were present in the enlarged spleen, suggesting that the spleen possessed natural killer (NK) cells. However, the splenic NK cell activity obtained in a 4h-51Cr release assay at 5 weeks after inoculation was below 5%, suggesting that the cytotoxic function of the NK cells was depressed. Bone marrow morphologically appeared not to be suppressed. Although the exact cause and mechanism of the progressive splenomegaly with prominent extramedullary haematopoiesis and severe suppression of the splenic NK cell activity remains speculative at present, it could be a unique manifestation of a systemic host-reaction reflected by the 9L-glioma continuously growing in the subcutaneous regions. This experiment could be a useful approach to the study of splenic and other reticuloendothelial roles in host-immunity in the tumour bearing state.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Abo T., Balch C. M. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol. 1981 Sep;127(3):1024–1029. [PubMed] [Google Scholar]
- Benda P., Someda K., Messer J., Sweet W. H. Morphological and immunochemical studies of rat glial tumors and clonal strains propagated in culture. J Neurosurg. 1971 Mar;34(3):310–323. doi: 10.3171/jns.1971.34.3.0310. [DOI] [PubMed] [Google Scholar]
- Elgert K. D., Farrar W. L. Suppressor cell activity in tumor-bearing mice. I. Dualistic inhibition by suppressor T lymphocytes and macrophages. J Immunol. 1978 Apr;120(4):1345–1353. [PubMed] [Google Scholar]
- Falk S., Seipelt G., Müller H., Stutte H. J. Immunohistochemical assessment of splenic lymphocyte and macrophage subpopulations in patients with gastric cancer. Cancer. 1989 Oct 15;64(8):1646–1651. doi: 10.1002/1097-0142(19891015)64:8<1646::aid-cncr2820640815>3.0.co;2-e. [DOI] [PubMed] [Google Scholar]
- Fujimoto S., Greene M. I., Sehon A. H. Regualtion of the immune response to tumor antigens. I. Immunosuppressor cells in tumor-bearing hosts. J Immunol. 1976 Mar;116(3):791–799. [PubMed] [Google Scholar]
- Fujimoto S., Greene M. I., Sehon A. H. Regulation of the immune response to tumor antigens. II. The nature of immunosuppressor cells in tumor-bearing hosts. J Immunol. 1976 Mar;116(3):800–806. [PubMed] [Google Scholar]
- Imaya H., Matsuura H., Kudo M., Nakazawa S. Suppression of splenic natural killer cell activity in rats with brain tumors. Neurosurgery. 1988 Jul;23(1):23–26. doi: 10.1227/00006123-198807000-00005. [DOI] [PubMed] [Google Scholar]
- Lanier L. L., Phillips J. H., Hackett J., Jr, Tutt M., Kumar V. Natural killer cells: definition of a cell type rather than a function. J Immunol. 1986 Nov 1;137(9):2735–2739. [PubMed] [Google Scholar]
- Sibbitt W. L., Jr, Bankhurst A. D., Jumonville A. J., Saiki J. H., Saiers J. H., Doberneck R. C. Defects in natural killer cell activity and interferon response in human lung carcinoma and malignant melanoma. Cancer Res. 1984 Feb;44(2):852–856. [PubMed] [Google Scholar]
- Steinhauer E. H., Doyle A. T., Reed J., Kadish A. S. Natural killer cytotoxic factor induction by K562 cells in patients with advanced cancer: correlation with production of interferon. J Natl Cancer Inst. 1985 Dec;75(6):1017–1023. [PubMed] [Google Scholar]
- Wrann M., Bodmer S., de Martin R., Siepl C., Hofer-Warbinek R., Frei K., Hofer E., Fontana A. T cell suppressor factor from human glioblastoma cells is a 12.5-kd protein closely related to transforming growth factor-beta. EMBO J. 1987 Jun;6(6):1633–1636. doi: 10.1002/j.1460-2075.1987.tb02411.x. [DOI] [PMC free article] [PubMed] [Google Scholar]