Skip to main content
NCBI home page
International Journal of Experimental Pathology logoLink to International Journal of Experimental Pathology
. 1993 Dec;74(6):547–552.

Ultrastructure of calcified muscle fibres at the implantation site of demineralized bone matrix gelatin.

K Yamashita 1, T Takagi 1
PMCID: PMC2002258  PMID: 8292552

Abstract

To clarify the mechanism of calcification during new bone formation induced by demineralized bone matrix gelatin (BMG), BMG was implanted into the skeletal muscle of Sprague-Dawley (SD) rats and histological changes were examined 1,2,3,5,7 and 10 days later. From day 2 after implantation, calcification was observed in muscle fibres near the implanted BMG. Calcification of the BMG ('acellular mineral deposition') was first seen on day 3 and in both, calcification increased. The calcification of muscle fibre was first seen in the sarcoplasmic reticulum (SR) and then in mitochondria or lipid droplet-like structures of satellite cells. The muscle mineral deposition occurred without osteogenic cells and matrix vesicles in the same way as 'acellular mineral deposition'.

Full text

PDF
547

Images in this article

548Figure 1
on p.548
548Figure 2
on p.548
549Figure 3
on p.549
550Figure 4
on p.550
550Figure 5
on p.550
551Figure 6
on p.551
551Figure 7
on p.551
551Figure 8
on p.551

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonucci E., Sadun R. An electron microscope study on experimental calcification of skeletal muscle. Clin Orthop Relat Res. 1972;88:197–217. doi: 10.1097/00003086-197210000-00031. [DOI] [PubMed] [Google Scholar]
  2. Katsura N. Nanospace theory for biomineralization. Dent Jpn (Tokyo) 1990;27(1):57–63. [PubMed] [Google Scholar]
  3. McClure J., Gardner D. L. The production of calcification in connective tissue and skeletal muscle using various chemical compounds. Calcif Tissue Res. 1976 Dec 22;22(2):129–135. doi: 10.1007/BF02010352. [DOI] [PubMed] [Google Scholar]
  4. McClure J. Skeletal muscle calcergy. J Pathol. 1982 May;137(1):1–12. doi: 10.1002/path.1711370102. [DOI] [PubMed] [Google Scholar]
  5. McClure J. The production of heterotopic calcification by certain chemical salts. J Pathol. 1980 May;131(1):21–33. doi: 10.1002/path.1711310103. [DOI] [PubMed] [Google Scholar]
  6. Ownby C. L., Odell G. V. Pathogenesis of skeletal muscle necrosis induced by tarantula venom. Exp Mol Pathol. 1983 Jun;38(3):283–296. doi: 10.1016/0014-4800(83)90069-2. [DOI] [PubMed] [Google Scholar]
  7. SELLE R. W., URIST M. R. Calcium deposits and new bone formation in muscle in rabbits. J Surg Res. 1961 Jul;1:132–141. doi: 10.1016/s0022-4804(61)80010-3. [DOI] [PubMed] [Google Scholar]
  8. SPEER D. P., URIST M. R. EXPERIMENTAL INTRACELLULAR CALCIFICATION OF MUSCLE. Clin Orthop Relat Res. 1965 Mar-Apr;39:213–231. [PubMed] [Google Scholar]
  9. Sampath T. K., Muthukumaran N., Reddi A. H. Isolation of osteogenin, an extracellular matrix-associated, bone-inductive protein, by heparin affinity chromatography. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7109–7113. doi: 10.1073/pnas.84.20.7109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Urist M. R. Bone: formation by autoinduction. Science. 1965 Nov 12;150(3698):893–899. doi: 10.1126/science.150.3698.893. [DOI] [PubMed] [Google Scholar]
  11. Urist M. R., Huo Y. K., Brownell A. G., Hohl W. M., Buyske J., Lietze A., Tempst P., Hunkapiller M., DeLange R. J. Purification of bovine bone morphogenetic protein by hydroxyapatite chromatography. Proc Natl Acad Sci U S A. 1984 Jan;81(2):371–375. doi: 10.1073/pnas.81.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Van Vleet J. F., Hall B. V., Simon J. Vitamin E deficiency. A sequential light and electron microscopic study of skeletal muscle degeneration in weanling rabbits. Am J Pathol. 1968 May;52(5):1067–1079. [PMC free article] [PubMed] [Google Scholar]
  13. Wozney J. M., Rosen V., Celeste A. J., Mitsock L. M., Whitters M. J., Kriz R. W., Hewick R. M., Wang E. A. Novel regulators of bone formation: molecular clones and activities. Science. 1988 Dec 16;242(4885):1528–1534. doi: 10.1126/science.3201241. [DOI] [PubMed] [Google Scholar]
  14. Yamashita K., Horisaka Y., Okamoto Y., Yoshimura Y., Matsumoto N., Kawada J., Takagi T. Effect of bupivacaine on muscle tissues and new bone formation induced by demineralized bone matrix gelatin. Acta Anat (Basel) 1991;141(1):1–7. doi: 10.1159/000147090. [DOI] [PubMed] [Google Scholar]
  15. Yamashita K., Takagi T. Ultrastructural observation of calcification preceding new bone formation induced by demineralized bone matrix gelatin. Acta Anat (Basel) 1992;143(4):261–267. doi: 10.1159/000147260. [DOI] [PubMed] [Google Scholar]

Articles from International Journal of Experimental Pathology are provided here courtesy of Wiley

RESOURCES