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International Journal of Experimental Pathology logoLink to International Journal of Experimental Pathology
. 1993 Dec;74(6):573–582.

A murine model of experimental autoimmune lens-induced uveitis using Klebsiella O3 lipopolysaccharide as a potent immunological adjuvant.

T Yokochi 1, Y Fujii 1, I Nakashima 1, J Asai 1, M Kiuchi 1, K Kojima 1, N Kato 1
PMCID: PMC2002260  PMID: 8292555

Abstract

Experimental autoimmune uveitis and finally panophthalmitis could be produced in mice by repeated immunization of syngeneic eyeball extract mixed with Klebsiella O3 lipopolysaccharide (KO3 LPS) as a powerful immunological adjuvant. No ocular lesions were produced in mice given eyeball extract emulsified in complete Freund's adjuvant (CFA), KO3 LPS alone or eyeball extract alone. Histopathological changes in the ocular lesions at the early stage after the second or tertiary immunization were characterized by infiltration with inflammatory cells in the ciliary body and iris. The iridocyclitis was followed by extensive infiltration of polymorphonuclear leucocytes (PMN) into the cornea, lens and the surrounding tissues after repeated immunization. Finally, these areas were replaced by granulomatous tissues infiltrated with mononuclear cells. On the other hand, the structure of the retina and sclera was partially preserved. Those mice exhibited production of autoantibodies and development of the delayed-type hypersensitivity (DTH) to syngeneic eyeball extract. Moreover, ocular lesions could be produced in normal recipient mice by transfer of sensitized lymphocytes from hyperimmunized mice. Therefore, it was suggested that the ocular lesions produced by repeated immunization with the mixture of eyeball extract and KO3 LPS were due to the autoimmune mechanism. This might be useful to model immunological phenomena in the pathogenesis of human phacoantigenic uveitis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fujii Y., Kato N., Kito J., Asai J., Yokochi T. Experimental autoimmune adrenalitis: a murine model for Addison's disease. Autoimmunity. 1992;12(1):47–52. doi: 10.3109/08916939209146129. [DOI] [PubMed] [Google Scholar]
  2. Fujii Y., Yokochi T., Nakashima I., Asai J., Kato N. A new mouse model for autoimmune orchitis. Med Microbiol Immunol. 1991;180(1):1–14. doi: 10.1007/BF00191695. [DOI] [PubMed] [Google Scholar]
  3. Howes E. L., Jr, McKay D. G. Comparison of the ocular effects of circulating endotoxin and immune complexes: role of vasoactive amines. J Immunol. 1975 Feb;114(2 Pt 2):734–737. [PubMed] [Google Scholar]
  4. Iwase K., Fujii Y., Nakashima I., Kato N., Fujino Y., Kawashima H., Mochizuki M. A new method for induction of experimental autoimmune uveoretinitis (EAU) in mice. Curr Eye Res. 1990 Mar;9(3):207–216. doi: 10.3109/02713689009044515. [DOI] [PubMed] [Google Scholar]
  5. Kato N., Ohta M., Kido N., Naito S., Nakashima I., Nagase F., Yokochi T. Strong adjuvanticity of bacterial lipopolysaccharides possessing the homopolysaccharides consisting of mannose as the O-specific polysaccharide chains. Med Microbiol Immunol. 1985;174(1):1–14. [PubMed] [Google Scholar]
  6. Kato T., Yamada H., Yokochi T., Nakashima I., Kato N. Experimental autoimmune hemolytic anemia in mice induced by repeated immunization with ultrasonicated syngeneic erythrocytes together with Klebsiella O3 lipopolysaccharide. Nihon Ketsueki Gakkai Zasshi. 1984 Jul;47(4):838–845. [PubMed] [Google Scholar]
  7. Kuriki J., Murakami H., Kakumu S., Sakamoto N., Yokochi T., Nakashima I., Kato N. Experimental autoimmune hepatitis in mice after immunization with syngeneic liver proteins together with the polysaccharide of Klebsiella pneumoniae. Gastroenterology. 1983 Mar;84(3):596–603. [PubMed] [Google Scholar]
  8. Marak G. E., Jr, Font R. L., Czawlytko L. N., Alepa F. P. Experimental lens-induced granulomatous endophthalmitis: preliminary histopathologic observations. Exp Eye Res. 1974 Oct;19(4):311–316. doi: 10.1016/0014-4835(74)90014-1. [DOI] [PubMed] [Google Scholar]
  9. Nakashima I., Yokochi T., Kato N., Asai J. Microbial adjuvant and autoimmunity. II. Production of lesions in mice immunized with syngeneic tissue extracts together with the capsular polysaccharide of Klebsiella pneumoniae. Microbiol Immunol. 1977;21(5):279–288. doi: 10.1111/j.1348-0421.1977.tb00288.x. [DOI] [PubMed] [Google Scholar]
  10. Ohta M., Mori M., Hasegawa T., Nagase F., Nakashima I., Naito S., Kato N. Further Studies of the polysaccharide of Klebsiella pneumoniae possessing strong adjuvanticity. I. Production of the adjuvant polysaccharide by noncapsulated mutant. Microbiol Immunol. 1981;25(9):939–948. doi: 10.1111/j.1348-0421.1981.tb00098.x. [DOI] [PubMed] [Google Scholar]
  11. Yamaki K., Ohta M., Nakashima I., Noda A., Asai J., Kato N. Microbial adjuvant and autoimmunity. IV. Production of lesions in the exocrine pancreas of mice by repeated injection of syngeneic pancreatic extract together with the capsular polysaccharide of Klebsiella pneumoniae. Microbiol Immunol. 1980;24(10):945–956. doi: 10.1111/j.1348-0421.1980.tb02900.x. [DOI] [PubMed] [Google Scholar]
  12. Yokochi T., Fukada M., Kawai M., Zhang Y. H., Jiang G. Z., Takahashi K. Novel adjuvant action of lipopolysaccharides that possess mannose homopolysaccharides as O-specific polysaccharides on immune responses to nonimmunogenic autoantigens in mice. Infect Immun. 1992 Nov;60(11):4953–4956. doi: 10.1128/iai.60.11.4953-4956.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Yokochi T., Ikeda H., Inoue Y., Kimura Y., Ito H., Fujii Y., Kato N. Characterization of autoantigens relevant to experimental autoimmune orchitis (EAO) in mice immunized with a mixture of syngeneic testis homogenate and Klebsiella O3 lipopolysaccharide. Am J Reprod Immunol. 1990 Jan-Feb;22(1-2):42–48. doi: 10.1111/j.1600-0897.1990.tb01030.x. [DOI] [PubMed] [Google Scholar]
  14. Yokochi T., Inoue Y., Kimura Y., Kato N. Strong interaction of lipopolysaccharides possessing the mannose homopolysaccharides with complement and its relation to adjuvant action. J Immunol. 1990 Apr 15;144(8):3106–3110. [PubMed] [Google Scholar]
  15. Yokochi T., Inoue Y., Yokoo J., Kimura Y., Kato N. Retention of bacterial lipopolysaccharide at the site of subcutaneous injection. Infect Immun. 1989 Jun;57(6):1786–1791. doi: 10.1128/iai.57.6.1786-1791.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yokochi T., Nakashima I., Kato N., Asai J. Microbial adjuvant and autoimmunity. III. Histological studies of development of experimental autoimmune thyroiditis in mice immunized with syngeneic thyroid extract together with the capsular polysaccharide of Klebsiella pneumoniae. Microbiol Immunol. 1978;22(10):619–630. doi: 10.1111/j.1348-0421.1978.tb00412.x. [DOI] [PubMed] [Google Scholar]
  17. Yokochi T., Oka K., Iwata H., Miyadai T., Kimura Y., Kato N. Characterization of autoantigens relevant to autoimmune ophthalmitis and thyroiditis in mice immunized with the syngeneic tissue extracts and Klebsiella O3 lipopolysaccharide. Microbiol Immunol. 1987;31(10):1025–1032. doi: 10.1111/j.1348-0421.1987.tb01335.x. [DOI] [PubMed] [Google Scholar]

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