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International Journal of Experimental Pathology logoLink to International Journal of Experimental Pathology
. 1992 Dec;73(6):741–750.

Induction of contact sensitivity by cell-associated immunocomplexes requires activation of the early complement components.

D Lio 1, G Sireci 1, F Gervasi 1, F Dieli 1, A Salerno 1
PMCID: PMC2002425  PMID: 1493103

Abstract

Lymph node cells collected from CBA/J mice 4 days after painting the skin with picryl chloride behave like antigen presenting cells and induce contact sensitivity when injected into naive recipient mice. The immunizing capacity of these '4-day' cells is due to T cells which carry on their membrane hapten-IgM immunocomplexes. Incubation of the cells with complement from mouse strains that express high C4 serum levels (C4H), abolishes their immunizing capacity. This effect is related to the activation of the early components of the classical complement pathway, as supported by experiments using C3 and C4-depleted or C5 and C6-genetically deficient mouse sera. The detection of different amounts of C3b and C4b on the surface of 4-day T cells after incubation with C4L and C4H sera supports the possibility that membrane bound activated complement components could modify the immunizing capacity of these cells. Results herein reported suggest that membrane-bound C3b and C4b are not per se inhibitory but interfere with the residual complement activating capacity of 4-day T cells. The role of complement activation by 4-day T cells is pivotal as complement depletion of recipient mice by cobra venom factor (CVF) inhibits the immunizing capacity of untreated 4-day T cells, while 4-day T cells treated with complement in vitro and injected together with C4a anaphylatoxin are able to immunize recipient mice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arvieux J., Yssel H., Colomb M. G. Antigen-bound C3b and C4b enhance antigen-presenting cell function in activation of human T-cell clones. Immunology. 1988 Oct;65(2):229–235. [PMC free article] [PubMed] [Google Scholar]
  2. Asherson G. L., Colizzi V., Watkins M. C. Immunogenic cells in the regional lymph nodes after painting with the contact sensitizers picryl chloride and oxazolone: evidence for the presence of IgM antibody on their surface. Immunology. 1983 Mar;48(3):561–569. [PMC free article] [PubMed] [Google Scholar]
  3. Bacle F., Haeffner-Cavaillon N., Laude M., Couturier C., Kazatchkine M. D. Induction of IL-1 release through stimulation of the C3b/C4b complement receptor type one (CR1, CD35) on human monocytes. J Immunol. 1990 Jan 1;144(1):147–152. [PubMed] [Google Scholar]
  4. Dieli F., Salerno A. Role of the fourth complement component (C4) in the regulation of contact sensitivity. I. Analysis in mice with high and low C4 levels. Cell Immunol. 1987 Apr 1;105(2):386–396. doi: 10.1016/0008-8749(87)90086-4. [DOI] [PubMed] [Google Scholar]
  5. Dieli F., Sireci G., Di Pace M. R., Salerno A. Role of the fourth complement component (C4) in the regulation of contact sensitivity. II. Qualitative differences between C4 molecules from high- and low-C4 mouse strains. Cell Immunol. 1989 Apr 1;119(2):243–251. doi: 10.1016/0008-8749(89)90241-4. [DOI] [PubMed] [Google Scholar]
  6. Dieli F., Sireci G., Lio D., Salerno A. Role of the fourth complement component (C4) in the regulation of contact sensitivity. III. In vivo effect of purified C4. Cell Immunol. 1989 Oct 1;123(1):236–243. doi: 10.1016/0008-8749(89)90283-9. [DOI] [PubMed] [Google Scholar]
  7. Dieli F., Sireci G., Lio D., Salerno A. The molecular basis of the low hemolytic activity of C4 molecules from low-C4 mice with IgM-coated erythrocytes. Eur J Immunol. 1989 Sep;19(9):1613–1618. doi: 10.1002/eji.1830190914. [DOI] [PubMed] [Google Scholar]
  8. Jungi T. W., Pepys M. B. Delayed hypersensitivity reactions to Listeria monocytogenes in rats decomplemented with cobra factor and in C5-deficient mice. Immunology. 1981 Jun;43(2):271–279. [PMC free article] [PubMed] [Google Scholar]
  9. Law S. K., Dodds A. W., Porter R. R. A comparison of the properties of two classes, C4A and C4B, of the human complement component C4. EMBO J. 1984 Aug;3(8):1819–1823. doi: 10.1002/j.1460-2075.1984.tb02052.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Paul L., Skanes V. M., Mayden J., Levine R. P. C4-mediated inhibition of immune precipitation and differences in inhibitory action of genetic variants, C4A3 and C4B1. Complement. 1988;5(3):110–119. doi: 10.1159/000463045. [DOI] [PubMed] [Google Scholar]
  11. Salerno A., Brai M., Dieli F., Colonna Romano G., Abrignani S., Colizzi V., Asherson G. L. Complement activation by cell-associated immune complexes in contact sensitivity. Cell Immunol. 1984 Dec;89(2):376–384. doi: 10.1016/0008-8749(84)90339-3. [DOI] [PubMed] [Google Scholar]

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