Yi-shin Su and J. Clark Lagarias (2007). Light-Independent Phytochrome Signaling Mediated by Dominant GAF Domain Tyrosine Mutants of Arabidopsis Phytochromes in Transgenic Plants. Plant Cell 19: 2124–2139.
Figure 3A should be labeled D instead of R.
Figure 3.
Phytochrome Chromophore Biosynthesis Is Required for the Gain-of-Function Activity of PHYBY276H.
(A) Transgenic plants with or without the hy1 mutation were grown in darkness for 6 d on sucrose-free media.
(B) Relative hypocotyl length of dark-grown seedlings in (A) normalized to that of Ler shows the differential activity of PHYBY276H in chromophore-replete phyA phyB and chromophore-deficient hy1 phyA phyB backgrounds. Values are mean ± sd (n = 50).
(C) Relative hypocotyl length of seedlings grown on sucrose-free media under 20 μmol m−2 s−1 continuous R. Mean hypocotyl lengths (± sd; n = 50) are normalized to those of dark-grown Ler seedlings. The shorter hypocotyl lengths of hy1/phyA, PHYB/hy1/phyA/phyB, and PHYBY276H/hy1/phyA/phyB plants compared with the hy1/phyA/phyB parent line indicate that sufficient bilin chromophore is present in the hy1 mutant background to maintain reduced but significant signaling activity of phyB and phyBY276H.
(D) Immunoblot analysis of PHYB protein level was performed as in Figure 1.
In Figure 7A, the labels have been corrected for the control lines. The control lines labeled phyB, phyA, phyA/phyB, and B have been changed to phyA, phyA/phyB, B#2, and B#14, respectively. The corrected figures appear below.
Figure 7.
Signaling Activities of phyBY276H and phyBY276Q Are Not Inhibited by Continuous FR Illumination.
(A) Comparative analysis of seedling development of 6-d-old plants grown under 20 μmol m−2 s−1 continuous FR. One representative line from transformation of phyA/phyB is shown.
(B) Comparative fluence response curves for hypocotyl growth indicate that phyBY276H-mediated growth suppression is FR light independent. Each data point represents the mean of 50 seedlings ± sd.
(C) Comparative analysis of seed germination phenotypes of the wild type and phyA phyB mutant transformed with genomic PHYBY276H or PHYBY276Q alleles.