Abstract
The activity of RNA polymerase has been determined in the nuclear fraction of normal mouse ovaries, 60-day-old preneoplastic intrasplenic ovarian grafts, and ovarian tumours developed after 7 months of ovary grafting into the spleen. In preneoplastic grafts, RNA polymerase activity corresponds to that of normal ovaries, while in ovarian tumours, the enzyme value was 2-3 times higher. Oestradiol injected for 10 days, acting as depressant of the host pituitary gonadotrophic potency, decreased the enzyme level in the grafts, whereas no change was observed in similarly treated tumours. These facts indicate that hormonal mechanisms regulating the genetic nuclear expression are operating only in the 2-month-old preneoplastic ovarian cell, while autonomy from these regulating mechanisms is achieved by 7-month-old tumour cells.
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- BRUZZONE S., BRANCATELLI A. Effect of estradiol on phosphorylase activity of intrasplenic ovarian graft in C57Bl mice. Nature. 1962 Aug 25;195:810–811. doi: 10.1038/195810a0. [DOI] [PubMed] [Google Scholar]
- Furth J. J., Ho P., Hopper D. K. The enzymatic synthesis of ribonucleic acid in animal tissue II. Comparison of the enzyme from normal bovine lymph node and bovine lymphosarcoma tissue. Cancer Res. 1966 Mar;26(3):435–437. [PubMed] [Google Scholar]
- GORSKI J. EARLY ESTROGEN EFFECTS ON THE ACTIVITY OF UTERINE RIBONUCLEIC ACID POLYMERASE. J Biol Chem. 1964 Mar;239:889–892. [PubMed] [Google Scholar]
- HANCOCK R. L., ZELIS R. F., SHAW M., WILLIAMS-ASHMAN H. G. Incorporation of ribonucleoside triphosphates into ribonucleic acid by nuclei of the prostate gland. Biochim Biophys Acta. 1962 Jan 22;55:257–260. doi: 10.1016/0006-3002(62)90967-8. [DOI] [PubMed] [Google Scholar]
- MILLER O. J., PFEIFFER C. A. Demonstration of increased gonadotrophic hormone production in castrated mice with intrasplenic ovarian grafts. Proc Soc Exp Biol Med. 1950 Oct;75(1):178–181. doi: 10.3181/00379727-75-18137. [DOI] [PubMed] [Google Scholar]
- Nakagawa S., White A. Acute decrease in RNA polymerase activity of rat thymus in response to cortisol injection. Proc Natl Acad Sci U S A. 1966 Apr;55(4):900–904. doi: 10.1073/pnas.55.4.900. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smellie R. M. Biochemistry of deoxyribonucleic acid and ribonucleic acid replication. Br Med Bull. 1965 Sep;21(3):195–202. doi: 10.1093/oxfordjournals.bmb.a070395. [DOI] [PubMed] [Google Scholar]
- TSUKADA K., LIEBERMAN I. LIVER NUCLEAR RIBONUCLEIC ACID POLYMERASE FORMED AFTER PARTIAL HEPATECTOMY. J Biol Chem. 1965 Apr;240:1731–1736. [PubMed] [Google Scholar]
- Van Dyke K., Katzman P. A. Effect of pregnant mare's serum (PMS) on RNA polymerase activity and protein synthesis in ovaries of immature rats. Endocrinology. 1968 Jul;83(1):107–112. doi: 10.1210/endo-83-1-107. [DOI] [PubMed] [Google Scholar]