Abstract
The growth of ascitic mouse plasmacytoma 5563 has been monitored by 51Cr-RBC dilutions within the peritoneal cavity together with differential cell counts and protein measurements.
Following the intraperitoneal transplantation of one million tumour cells, there is a latency period of 4 days, possibly explained as only a 5% survival of the inoculum. This is followed by logarithmic growth from 4 to 8 days with a doubling time of 18 hours, always similar in different animals. Eventually after 8 days and after about 100 million cells an asymptote is reached which is due to a fistula-like communication between blood-stream and peritoneal cavity. This terminal phase (9-10 days) is rapidly followed by death of the mouse.
Reliable tumour cell counts can only be achieved between 4 and 8 days.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BASERGA R. Mitotic cycle of ascites tumor cells. Arch Pathol. 1963 Feb;75:156–161. [PubMed] [Google Scholar]
- Fakhri O., Hobbs J. R. The serum paraprotein level related to the number of plasmacytoma-5536 cells in C3H mice. Br J Cancer. 1970 Jun;24(2):395–397. doi: 10.1038/bjc.1970.46. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frindel E., Malaise E. P., Alpen E., Tubiana M. Kinetics of cell proliferation of an experimental tumor. Cancer Res. 1967 Jun;27(6):1122–1131. [PubMed] [Google Scholar]
- KLEIN G., REVESZ L. Quantitative studies on the multiplication of neoplastic cells in vivo. I. Growth curves of the Ehrlich and MC1M ascites tumors. J Natl Cancer Inst. 1953 Oct;14(2):229–277. doi: 10.1093/jnci/14.2.229. [DOI] [PubMed] [Google Scholar]
- Lala P. K., Patt H. M. Cytokinetic analysis of tumor growth. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1735–1742. doi: 10.1073/pnas.56.6.1735. [DOI] [PMC free article] [PubMed] [Google Scholar]