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. 1972 Aug;26(4):304–314. doi: 10.1038/bjc.1972.41

The Effect of Cyclophosphamide on the Growth and Cellular Kinetics of a Transplantable Rat Fibrosarcoma

Sandra Peel, Diana M Cowen
PMCID: PMC2008649  PMID: 5071192

Abstract

The gross response of a transplantable fibrosarcoma (RIB5) in rats treated with a single dose of 10, 50, 100 or 200 mg/kg body weight of cyclophosphamide (CP) is reported. The variation in mitotic and tritiated thymidine (3H-TdR) labelling indices after a single dose of 100 mg/kg CP was studied. Examination of chromosome spreads has shown the time course of the visible damage to DNA caused by CP and a transient arrest of cells in G2 was demonstrated by microdensitometry. The effects of 100 mg/kg body weight CP given in 2 equal doses separated by various time intervals were examined in an attempt to relate tumour response to its altered cellular state at the time of the second dose.

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Selected References

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