Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1973 Apr;27(4):316–322. doi: 10.1038/bjc.1973.38

The Concentration of Free and Conjugated 3-Hydroxyanthranilic Acid in the Urine of Bladder Tumour Patients Before and After Therapy, Measured with an Enzymatic Method

F A G Teulings, W Fokkens, J G A H Kaalen, B van der Werf-Messing
PMCID: PMC2008782  PMID: 4701704

Abstract

The basal concentration of the tryptophan metabolite 3-hydroxyanthranilic acid (3OHA), which has carcinogenic properties, was measured with an enzymatic method of determination which allowed separate measurement of free and conjugated 3OHA. The concentration of free 3OHA in untreated bladder cancer patients was significantly (P <0·001) higher than in a healthy control group, but after local therapy the concentration was significantly lower than before treatment (P <0·01). The concentration of conjugated 3OHA was nearly constant in the three groups. It was concluded that other factors than a genetic determined abnormality might be operating in bladder cancer patients which could lead to an abnormal concentration of 3OHA in their urine.

Full text

PDF
316

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENASSI C. A., PERISSINOTTO B., ALLEGRI G. THE METABOLISM OF TRYPTOPHAN IN PATIENTS WITH BLADDER CANCER AND OTHER UROLOGICAL DISEASES. Clin Chim Acta. 1963 Nov;8:822–831. doi: 10.1016/0009-8981(63)90003-2. [DOI] [PubMed] [Google Scholar]
  2. BOYLAND E., WILLIAMS D. C. The metabolism of tryptophan. 2. The metabolism of tryptophan in patients suffering from cancer of the bladder. Biochem J. 1956 Nov;64(3):578–582. doi: 10.1042/bj0640578. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BROWN R. R., PRICE J. M. Quantitative studies on metabolites of tryptophan in the urine of the dog, cat, rat, and man. J Biol Chem. 1956 Apr;219(2):985–997. [PubMed] [Google Scholar]
  4. BROWN R. R., PRICE J. M., SATTER E. J., WEAR J. B. The metabolism of tryptophan in patients with bladder cancer. Acta Unio Int Contra Cancrum. 1960;16:299–303. [PubMed] [Google Scholar]
  5. Bryan G. T. The role of urinary tryptophan metabolites in the etiology of bladder cancer. Am J Clin Nutr. 1971 Jul;24(7):841–847. doi: 10.1093/ajcn/24.7.841. [DOI] [PubMed] [Google Scholar]
  6. DUNNING W. F., CURTIS M. R., MAUN M. E. The effect of added dietary tryptophane on the occurrence of 2-acetylaminofluorene-induced liver and bladder cancer in rats. Cancer Res. 1950 Jul;10(7):454–459. [PubMed] [Google Scholar]
  7. Heeley A. F. The effect of pyridoxine on tryptophan metabolism in phenylketonuria. Clin Sci. 1965 Dec;29(3):465–473. [PubMed] [Google Scholar]
  8. Kochen W., Hochberg K. Untersuchungen über den Tryptophan-Stoffwechsel beim Blasen-Carcinom. Z Krebsforsch. 1970;73(3):251–264. [PubMed] [Google Scholar]
  9. PRICE J. M., BROWN R. R. Studies on the etiology of carcinoma of the urinary bladder. Acta Unio Int Contra Cancrum. 1962;18:684–688. [PubMed] [Google Scholar]
  10. Radomski J. L., Glass E. M., Deichmann W. B. Transitional cell hyperplasia in the bladders of dogs fed DL-tryptophan. Cancer Res. 1971 Nov;31(11):1690–1694. [PubMed] [Google Scholar]
  11. Rose D. P. Aspects of tryptophan metabolism in health and disease: a review. J Clin Pathol. 1972 Jan;25(1):17–25. doi: 10.1136/jcp.25.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schievelbein H., Buchfink E. An enzymatic method for the estimation of free and conjugated 3-hydroxyanthranilic acid in urine. Clin Chim Acta. 1967 Nov;18(2):291–295. doi: 10.1016/0009-8981(67)90171-4. [DOI] [PubMed] [Google Scholar]
  13. Watanabe M., Minegishi K. Studies on carcinogenic tryptophan metabolites. II. Enzymatic formation and hydrolysis of sulfuric ester of 3-hydroxyanthranilic acid. Biochem Pharmacol. 1972 May 1;21(9):1347–1356. doi: 10.1016/0006-2952(72)90296-1. [DOI] [PubMed] [Google Scholar]
  14. Watanabe M., Okubo K., Tamura Z. Studies on carcinogenic tryptophan metabolites. I. Enzymatic formation and hydrolysis of glucuronide of 3-hydroxyanthranilic acid. Biochem Pharmacol. 1972 May 1;21(9):1337–1346. doi: 10.1016/0006-2952(72)90295-x. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES