Skip to main content
British Journal of Cancer logoLink to British Journal of Cancer
. 1973 Apr;27(4):283–290. doi: 10.1038/bjc.1973.35

The Effects of Aflatoxin B1 in vivo on Membrane—Ribosome Association

D J Williams, R P Clark, B R Rabin
PMCID: PMC2008791  PMID: 4144962

Abstract

The effects of aflatoxin B1 on the endoplasmic reticulum of rat liver has been examined in vivo. Electron microscopy has shown a disorganization and degranulation of rough surfaced membrane under these conditions and evidence is presented that this is a primary effect of the toxin, and results from the direct attack of the aflatoxin on the steroid-dependent ribosome binding sites on the membrane. A technique is described by which the presence of degranulated rough membrane may be detected in microsomal preparations.

Full text

PDF
283

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABEL L. L., LEVY B. B., BRODIE B. B., KENDALL F. E. A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity. J Biol Chem. 1952 Mar;195(1):357–366. [PubMed] [Google Scholar]
  2. ARCOS J. C., CONNEY A. H., BUU-HOI N. P. Induction of microsomal enzyme synthesis by polycyclic aromatic hydrocarbons of different molecular sizes. J Biol Chem. 1961 May;236:1291–1296. [PubMed] [Google Scholar]
  3. BAGLIO C. M., FARBER E. CORRESPONDENCE BETWEEN RIBOSOME AGGREGATION PATTERNS IN RAT LIVER HOMOGENATES AND IN ELECTRON MICROGRAPHS FOLLOWING ADMINISTRATION OF ETHIONINE. J Mol Biol. 1965 Jun;12:466–467. doi: 10.1016/s0022-2836(65)80269-8. [DOI] [PubMed] [Google Scholar]
  4. BUTLER W. H., BARNES J. M. TOXIC EFFECTS OF GROUNDNUT MEAL CONTAINING AFLATOXIN TO RATS AND GUINEA-PIGS. Br J Cancer. 1963 Dec;17:699–710. doi: 10.1038/bjc.1963.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Blyth C. A., Freedman R. B., Rabin B. R. The effects of aflatoxin B1 on the sex-specific binding of steroid hormones to microsomal membranes of rat liver. Eur J Biochem. 1971 Jun 29;20(4):580–586. doi: 10.1111/j.1432-1033.1971.tb01430.x. [DOI] [PubMed] [Google Scholar]
  6. Butler W. H., Barnes J. M. Carcinogenic action of groundnut meal containing aflatoxin in rats. Food Cosmet Toxicol. 1968 Aug;6(2):135–141. doi: 10.1016/0015-6264(68)90194-6. [DOI] [PubMed] [Google Scholar]
  7. Butler W. H. Early hepatic parenchymal changes induced in the rat by aflatoxin B1. Am J Pathol. 1966 Jul;49(1):113–128. [PMC free article] [PubMed] [Google Scholar]
  8. Clifford J. I., Rees K. R., Stevens M. E. The effect of the aflatoxins B1, G1, and G2 on protein and nucleic acid synthesis in rat liver. Biochem J. 1967 Apr;103(1):258–261. doi: 10.1042/bj1030258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Flaks B. Changes in the fine structure of rat hepatocytes during the early phases of chronic 2-acetylaminofluorene intoxication. Chem Biol Interact. 1970 Aug;2(2):129–150. doi: 10.1016/0009-2797(70)90045-1. [DOI] [PubMed] [Google Scholar]
  10. Harley E. H., Rees K. R. A comparative study of the effect of aflatoxin B and actinomycin D on HeLa cells. Biochem J. 1969 Sep;114(2):289–298. doi: 10.1042/bj1140289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Harris C. C., Sporn M. B., Kaufman D. G., Smith J. M., Baker M. S., Saffiotti U. Acute ultrastructural effects of benzo[a]pyrene and ferric oxide the hamster tracheobronchial epithelium. Cancer Res. 1971 Dec;31(12):1977–1989. [PubMed] [Google Scholar]
  12. James D. W., Rabin B. R., Williams D. J. Role of steroid hormones in the interaction of polysomes with endoplasmic reticulum. Nature. 1969 Oct 25;224(5217):371–372. doi: 10.1038/224371a0. [DOI] [PubMed] [Google Scholar]
  13. Ketterer B., Holt S. J., Ross-Mansell P. The effect of a single intraperitoneal dose of the hepatocarcinogen 4-dimethylaminoazobenzene on the rough-surfaced endoplasmic reticulum of the liver of the rat. Biochem J. 1967 Jun;103(3):692–698. doi: 10.1042/bj1030692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Magee P. N., Swann P. F. Nitroso compounds. Br Med Bull. 1969 Sep;25(3):240–244. doi: 10.1093/oxfordjournals.bmb.a070711. [DOI] [PubMed] [Google Scholar]
  16. Orrenius S., Ericsson J. L. Enzyme-membrane relationship in phenobarbital induction of synthesis of drug-metabolizing enzyme system and proliferation of endoplasmic membranes. J Cell Biol. 1966 Feb;28(2):181–198. doi: 10.1083/jcb.28.2.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. PHILLIPS A. H., LANGDON R. G. Hepatic triphosphopyridine nucleotide-cytochrome c reductase: isolation, characterization, and kinetic studies. J Biol Chem. 1962 Aug;237:2652–2660. [PubMed] [Google Scholar]
  18. PORTER K. R., BRUNI C. An electron microscope study of the early effects of 3'-Me-DAB on rat liver cells. Cancer Res. 1959 Nov;19:997–1009. [PubMed] [Google Scholar]
  19. Pong R. S., Wogan G. N. Time course of alterations of rat liver polysome profiles induced by aflatoxin B 1 . Biochem Pharmacol. 1969 Oct;18(10):2357–2361. doi: 10.1016/0006-2952(69)90350-5. [DOI] [PubMed] [Google Scholar]
  20. Rolleston F. S. The binding of ribosomal subunits to endoplasmic reticulum membranes. Biochem J. 1972 Sep;129(3):721–731. doi: 10.1042/bj1290721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schabort J. C., Steyn M. Substrate and phenobarbital inducible aflatoxin-4-hydroxylation and aflatoxin metabolism by rat liver microsomes. Biochem Pharmacol. 1969 Sep;18(9):2241–2252. doi: 10.1016/0006-2952(69)90331-1. [DOI] [PubMed] [Google Scholar]
  22. Sunshine G. H., Williams D. J., Rabin B. R. Role for steroid hormones in the interaction of ribosomes with the endoplasmic membranes of rat liver. Nat New Biol. 1971 Mar 31;230(13):133–136. doi: 10.1038/newbio230133a0. [DOI] [PubMed] [Google Scholar]
  23. Svoboda D., Higginson J. A comparison of ultrastructural changes in rat liver due to chemical carcinogens. Cancer Res. 1968 Sep;28(9):1703–1733. [PubMed] [Google Scholar]
  24. Süss R., Blobel G., Pitot H. C. Rat liver and hepatoma polysome-membrane interaction in vitro. Biochem Biophys Res Commun. 1966 May 3;23(3):299–304. doi: 10.1016/0006-291x(66)90545-6. [DOI] [PubMed] [Google Scholar]
  25. Williams D. J., Gurari D., Rabin B. R. The effects of ribosomes on the activity of a membrane bound enzyme catalysing thiol-disulphide interchange. FEBS Lett. 1968 Dec;2(2):133–135. doi: 10.1016/0014-5793(68)80123-1. [DOI] [PubMed] [Google Scholar]
  26. Williams D. J., Rabin B. R. Distruption by carcinogens of the hormone dependent association of membranes with polysomes. Nature. 1971 Jul 9;232(5306):102–105. doi: 10.1038/232102a0. [DOI] [PubMed] [Google Scholar]
  27. Williams D. J., Rabin B. R., Kisilevsky R. Endoplasmic membrane degranulation in vivo as a result of ethionine intoxication. FEBS Lett. 1972 Oct 1;26(1):245–248. doi: 10.1016/0014-5793(72)80583-0. [DOI] [PubMed] [Google Scholar]
  28. Williams D. J., Rabin B. R. The effects of aflatoxin B(1) and steroid hormones on polysome binding to microsomal membranes as measured by the activity of an enzyme catalysing disulphide interchange. FEBS Lett. 1969 Jul;4(2):103–107. doi: 10.1016/0014-5793(69)80207-3. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES