Abstract
The presence of Y bodies and similar fluorescent chromocentres in the interphase cells of 73 benign and malignant neoplastic lesions of males, and 69 of females, has been assessed in preparations stained with quinacrine dihydrochloride. In the male, Y bodies were usually present, although none was seen in 16 of the 65 malignant tumours. Sometimes the Y body was present in duplicate, for example, in some regions of a benign polyp of the colon and generally in 10 of the malignant tumours. The series includes 5 seminomata and 12 malignant testicular teratomata, all of which were positive for Y bodies.
The tumours of females failed to show similar bodies, with 5 exceptions: one of 13 carcinomata of the ovary showed a body resembling the Y body in about half the cells (however, a similar body was seen in lymphocytes from this case) while a further carcinoma of probable ovarian origin, and 3 of 13 ovarian dermoids, showed a similar body though in less than 20% of the cells.
Although quinacrine fluorescence studies on interphase tumour cells may be of value in suggesting the presence or absence of Y chromosomes, it is desirable that these studies be supplemented by the investigation of the fluorescence pattern of the metaphase chromosomes.
Full text
PDF






Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Akesson H. O., Forssman H., Wahlström J. Fluorescence study of interphase nuclei and double Y chromosomes. Hereditas. 1971;69(2):213–216. doi: 10.1111/j.1601-5223.1971.tb02435.x. [DOI] [PubMed] [Google Scholar]
- Atkin N. B. Triple sex chromatin, and other sex chromatin anomalies, in tumours of females. Br J Cancer. 1967 Mar;21(1):40–47. doi: 10.1038/bjc.1967.6. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Atkin N. B. Y chromosomes and quinacrine fluorescence technique. Br Med J. 1970 Oct 10;4(5727):118–118. doi: 10.1136/bmj.4.5727.118. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BERENBAUM M. C., BROWN I. N. DOSE-RESPONSE RELATIONSHIPS FOR AGENTS INHIBITING THE IMMUNE RESPONSE. Immunology. 1964 Jan;7:65–71. [PMC free article] [PubMed] [Google Scholar]
- Bagshawe K. D., Currie G. A. Immunogenicity of L 1210 murine leukaemia cells after treatment with neuraminidase. Nature. 1968 Jun 29;218(5148):1254–1255. doi: 10.1038/2181254a0. [DOI] [PubMed] [Google Scholar]
- Baker M. C., Atkin N. B. Chromosome abnormalities in polyps and carcinomas of the large bowel. Proc R Soc Med. 1970;63 (Suppl):9–10. [PMC free article] [PubMed] [Google Scholar]
- Bekesi J. G., St-Arneault G., Holland J. F. Increase of leukemia L1210 immunogenicity by Vibrio cholerae neuraminidase treatment. Cancer Res. 1971 Dec;31(12):2130–2132. [PubMed] [Google Scholar]
- Cormack D. Effect of enzymatic removal of cell surface sialic acid on the adherence of Walker 256 tumor cells to mesothelial membrane. Cancer Res. 1970 May;30(5):1459–1466. [PubMed] [Google Scholar]
- Currie G. A., Bagshawe K. D. The role of sialic acid in antigenic expression: further studies of the Landschütz ascites tumour. Br J Cancer. 1968 Dec;22(4):843–853. doi: 10.1038/bjc.1968.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Currie G. A., Bagshawe K. D. Tumour specific immunogenicity of methylcholanthrene-induced sarcoma cells after incubation in neuraminidase. Br J Cancer. 1969 Mar;23(1):141–149. doi: 10.1038/bjc.1969.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leiter J., Abbott B. J., Schepartz S. A. Screening data from the Cancer Chemotherapy National Service Center Screening Laboratories. XXI. Can Res Suppl. 1964 Dec;:1093–1353. [PubMed] [Google Scholar]
- Litton L. E., Hollander D. H., Borgaonkar D. S., Frost J. K. Y-chromatin of interphase cancer cells, a preliminary study. Acta Cytol. 1972 Sep-Oct;16(5):404–407. [PubMed] [Google Scholar]
- Mihich E. Combined effects of chemotherapy and immunity against leukemia L1210 in DBA-2 mice. Cancer Res. 1969 Apr;29(4):848–854. [PubMed] [Google Scholar]
- Pearson P. L., Bobrow M., Vosa C. G. Technique for identifying Y chromosomes in human interphase nuclei. Nature. 1970 Apr 4;226(5240):78–80. doi: 10.1038/226078a0. [DOI] [PubMed] [Google Scholar]
- ROSENBERG L. T., TACHIBANA D. K. Activity of mouse complement. J Immunol. 1962 Dec;89:861–867. [PubMed] [Google Scholar]
- Ray P. K., Gewurz H., Simmons R. L. Complement sensitivity of neuraminidase-treated lymphoid cells. Transplantation. 1971 Oct;12(4):327–329. doi: 10.1097/00007890-197110000-00018. [DOI] [PubMed] [Google Scholar]
- Robinson J. A., Buckton K. E. Quinacrine fluorescence of variant and abnormal human Y chromosomes. Chromosoma. 1971;35(3):342–352. doi: 10.1007/BF00326283. [DOI] [PubMed] [Google Scholar]
- Sanford B. H. An alteration in tumor histocompatibility induced by neuraminidase. Transplantation. 1967 Sep 5;5(5):1273–1279. doi: 10.1097/00007890-196709000-00005. [DOI] [PubMed] [Google Scholar]
- Sanford B. H., Codington J. F. Further studies on the effect of neuraminidase on tumor cell transplantability. Tissue Antigens. 1971;1(4):153–161. doi: 10.1111/j.1399-0039.1971.tb00092.x. [DOI] [PubMed] [Google Scholar]
- Sethi K. K., Brandis H. In vitro cytotoxicity of normal serum factor(s) on neuraminidase-treated Ehrlich ascites tumor cells and murine leukemia L 1210 cells. Z Immunitatsforsch Exp Klin Immunol. 1972 May;143(4):426–429. [PubMed] [Google Scholar]
- Skipper H. E., Schabel F. M., Jr, Wilcox W. S. Experimental evaluation of potential anticancer agents. XXI. Scheduling of arabinosylcytosine to take advantage of its S-phase specificity against leukemia cells. Cancer Chemother Rep. 1967 Jun;51(3):125–165. [PubMed] [Google Scholar]
- Woodruff J. J., Gesner B. M. The effect of neuraminidase on the fate of transfused lymphocytes. J Exp Med. 1969 Mar 1;129(3):551–567. doi: 10.1084/jem.129.3.551. [DOI] [PMC free article] [PubMed] [Google Scholar]