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. 1973 Feb;27(2):173–182. doi: 10.1038/bjc.1973.21

Human Peripheral Lung Tumours: Light and Electron Microscopic Correlation

Franco Mollo, Maria G Canese, Onofrio Campobasso
PMCID: PMC2008850  PMID: 4348471

Abstract

Thirteen human peripheral lung tumours have been studied in both light and electron microscopy. They were classified as epidermoid carcinoma, mucus-secreting cell adenocarcinoma, and alveolar cell adenocarcinoma, the latter made up of granular pneumocytes. Alveolar cell cancer, as defined by ultrastructural features, could assume different gross histological patterns in light microscopy, and therefore electron microscopy is required for its identification.

Since neither squamous nor mucous metaplasia was observed in any alveolar cell tumour, it is tentatively suggested that all peripheral lung tumours which lack these features may be derived from granular pneumocytes, irrespective of whether they appear to be adenocarcinomata or large cell carcinomata when examined by light microscopy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adamson J. S., Senior R. M., Merrill T. Alveolar cell carcinoma. An electron microscopic study. Am Rev Respir Dis. 1969 Oct;100(4):550–557. doi: 10.1164/arrd.1969.100.4.550. [DOI] [PubMed] [Google Scholar]
  2. Berkheiser S. W. Carcinoma in situ of the lung of peripheral (bronchiolar) origin. Am J Clin Pathol. 1966 Sep;46(3):315–321. doi: 10.1093/ajcp/46.3.315. [DOI] [PubMed] [Google Scholar]
  3. Brooks R. E. Pulmonary adenoma of strain A mice: an electron microscopic study. J Natl Cancer Inst. 1968 Sep;41(3):719–742. [PubMed] [Google Scholar]
  4. Campobasso O. The characteristics of peripheral lung tumours that suggest their bronchiolo-alveolar origin. Br J Cancer. 1968 Dec;22(4):655–662. doi: 10.1038/bjc.1968.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coalson J. J., Mohr J. A., Pirtle J. K., Dee A. L., Rhoades E. R. Electron microscopy of neoplasms in the lung with special emphasis on the alveolar cell carcinoma. Am Rev Respir Dis. 1970 Feb;101(2):181–197. doi: 10.1164/arrd.1970.101.2.181. [DOI] [PubMed] [Google Scholar]
  6. FRIEDBERG E. C. GIANT-CELL CARCINOMA OF THE LUNG: A DEDIFFERENTIATED ADENOCARCINOMA. Cancer. 1965 Feb;18:259–264. doi: 10.1002/1097-0142(196502)18:2<259::aid-cncr2820180219>3.0.co;2-f. [DOI] [PubMed] [Google Scholar]
  7. Flaks B., Flaks A. Fine structure of murine pulmonary adenomata induced by carcinogen treatment in organ culture. Cancer Res. 1969 Oct;29(10):1781–1789. [PubMed] [Google Scholar]
  8. Geller S. A., Toker C. Pulmonary adenomatosis and peripheral adenocarcinoma of the lung. An ultrastructural demonstration of common morphologic features. Arch Pathol. 1969 Aug;88(2):148–154. [PubMed] [Google Scholar]
  9. Greene J. G., Brown A. L., Jr, Divertie M. B. Fine structure of squamous cell carcinoma of the lung. Mayo Clin Proc. 1969 Feb;44(2):85–95. [PubMed] [Google Scholar]
  10. Hattori S., Matsuda M., Tateishi R., Terazawa T. Electron microscopic studies on human lung cancer cells. Gan. 1967 Jun;58(3):283–290. [PubMed] [Google Scholar]
  11. Herman D. L., Bullock W. K., Waken J. K. Giant cell adenocarcinoma of the lung. Cancer. 1966 Oct;19(10):1337–1346. doi: 10.1002/1097-0142(196610)19:10<1337::aid-cncr2820191003>3.0.co;2-3. [DOI] [PubMed] [Google Scholar]
  12. Johnston W. W., Ginn F. L., Amatulli J. M. Light and electron microscopic observations on malignant cells in cerebrospinal fluid from metastatic alveolar cell carcinoma. Acta Cytol. 1971 Jul-Aug;15(4):365–371. [PubMed] [Google Scholar]
  13. KLAERNER P., GIESEKING R. [On the ultrastructure of lung tumors in mice]. Z Krebsforsch. 1960;64:7–21. [PubMed] [Google Scholar]
  14. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lisa J. R., Trinidad S., Rosenblatt M. B. Site of origin, histogenesis, and cytostructure of bronchogenic carcinoma. Am J Clin Pathol. 1965 Oct;44(4):375–384. doi: 10.1093/ajcp/44.4.375. [DOI] [PubMed] [Google Scholar]
  16. MEYER E. C., LIEBOW A. A. RELATIONSHIP OF INTERSTITIAL PNEUMONIA HONEYCOMBING AND ATYPICAL EPITHELIAL PROLIFERATION TO CANCER OF THE LUNG. Cancer. 1965 Mar;18:322–351. doi: 10.1002/1097-0142(196503)18:3<322::aid-cncr2820180310>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  17. Mollo F., Canese M. G., Stramignoni A. Nuclear sheets in epithelial and connective tissue cells. Nature. 1969 Mar 1;221(5183):869–870. doi: 10.1038/221869a0. [DOI] [PubMed] [Google Scholar]
  18. Nagaishi C., Okada Y., Daido S., Genka K., Ikeda S., Kitano M. Electron microscopic observations of the human lung cancer. Exp Med Surg. 1965;23(2):177–202. [PubMed] [Google Scholar]
  19. Nash G., Langlinais P. C., Greenawald K. A. Alveolar cell carcinoma: does it exist? Cancer. 1972 Feb;29(2):322–326. doi: 10.1002/1097-0142(197202)29:2<322::aid-cncr2820290209>3.0.co;2-t. [DOI] [PubMed] [Google Scholar]
  20. Nisbet D. I., Mackay J. M., Smith W., Gray E. W. Ultrastructure of sheep pulmonary adenomatosis (Jaagsiekte). J Pathol. 1971 Mar;103(3):157–162. doi: 10.1002/path.1711030304. [DOI] [PubMed] [Google Scholar]
  21. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Razzuk M. A., Race G. J., Lynn J. A., Martin J. A., Urschel H. C., Jr, Paulson D. L. Observations on ultrastructural morphology of bronchogenic carcinoma. J Thorac Cardiovasc Surg. 1970 Apr;59(4):581–587. [PubMed] [Google Scholar]
  24. SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. SASAKI M., HAYASHI N., YAMORI T. ELECTRON MICROSCOPIC STUDIES ON HUMAN PULMONARY CARCINOMA. Gan. 1964 Apr;55:109–115. [PubMed] [Google Scholar]
  26. SCHULZ H. Some new observations on the submicroscopic pathology of the lung. Pulmonary adenomatosis and fat embolism. Lab Invest. 1963 Jun;12:616–627. [PubMed] [Google Scholar]
  27. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]

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