Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1973 Nov;28(5):389–399. doi: 10.1038/bjc.1973.164

Immunogenicity of Rat Hepatoma Membrane Fractions

R W Baldwin, M J Embleton, M Moore
PMCID: PMC2008927  PMID: 4357132

Abstract

The principal expression of immunity elicited in syngeneic rats immunized with rat hepatoma membrane fractions was the development of a tumour specific antibody response. This antibody was demonstrable by membrane immunofluorescence staining of viable hepatoma cells in suspension and the sera exhibited complement dependent cytotoxicity for cultured hepatoma cells. In the absence of complement, however, membrane immune sera were highly “blocking”, protecting plated hepatoma cells from attack by sensitized lymph node cells. The cell mediated immune response elicited by hepatoma membrane immunization was weak, as evaluated by the colony inhibitory activity of lymph node cells for hepatoma cells in vitro or the adoptive transfer of immunity with peritoneal exudate cells. Correlated with this overall pattern of immune response, membrane immunization did not elicit tumour rejection reactions. These findings are relevant to current views that humoral factors operate antagonistically to limit cell mediated immunity to tumours. A further relevant feature was the observation that membrane immunization, eliciting a prominent humoral immune reaction, conditioned the recipients so that they subsequently failed to elicit a tumour rejection immunity on treatment with irradiated tumour cells.

Full text

PDF
389

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander P., Bensted J., Delorme E. J., Hall J. G., Hodgett J. The cellular immune response to primary sarcomata in rats. II. Abnormal responses of nodes draining the tumour. Proc R Soc Lond B Biol Sci. 1969 Nov 18;174(1035):237–251. doi: 10.1098/rspb.1969.0090. [DOI] [PubMed] [Google Scholar]
  2. Baldwin R. W., Barker C. R. Demonstration of tumour-specific humoral antibody against aminoazo dye-induced rat hepatomata. Br J Cancer. 1967 Dec;21(4):793–800. doi: 10.1038/bjc.1967.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baldwin R. W., Barker C. R., Embleton M. J., Glaves D., Moore M., Pimm M. V. Demonstration of cell-surface antigens on chemically induced tumors. Ann N Y Acad Sci. 1971 Jun 21;177:268–278. doi: 10.1111/j.1749-6632.1971.tb35056.x. [DOI] [PubMed] [Google Scholar]
  4. Baldwin R. W., Barker C. R. Tumour-specific antigenicity of aminoazo-dye-induced rat hepatomas. Int J Cancer. 1967 Jul 15;2(4):355–364. doi: 10.1002/ijc.2910020410. [DOI] [PubMed] [Google Scholar]
  5. Baldwin R. W., Bowen J. G., Price M. R. Detection of circulating hepatoma D23 antigen and immune complexes in tumour bearer serum. Br J Cancer. 1973 Jul;28(1):16–24. doi: 10.1038/bjc.1973.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Baldwin R. W., Embleton M. J. Demonstration by colony inhibition methods of cellular and humoral immune reactions to tumour-specific antigens associated with aminoazo-dye-induced rat hepatomas. Int J Cancer. 1971 Jan 15;7(1):17–25. doi: 10.1002/ijc.2910070103. [DOI] [PubMed] [Google Scholar]
  7. Baldwin R. W., Embleton M. J. Detection and isolation of tumour-specific antigen associated with a spontaneously arising rat mammary carcinoma. Int J Cancer. 1970 Nov 15;6(3):373–382. doi: 10.1002/ijc.2910060307. [DOI] [PubMed] [Google Scholar]
  8. Baldwin R. W., Embleton M. J., Robins R. A. Cellular and humoral immunity to rat hepatoma-specific antigens correlated with tumour status. Int J Cancer. 1973 Jan 15;11(1):1–10. doi: 10.1002/ijc.2910110102. [DOI] [PubMed] [Google Scholar]
  9. Baldwin R. W., Harris J. R., Price M. R. Fractionation of plasma membrane-associated tumor-specific antigen from an aminoazo dye-induced rat hepatoma. Int J Cancer. 1973 Mar 15;11(2):385–397. doi: 10.1002/ijc.2910110216. [DOI] [PubMed] [Google Scholar]
  10. Baldwin R. W., Moore M. Isolation of membrane-associated tumour-specific antigen from an aminoazo-dye-induced rat hepatoma. Int J Cancer. 1969 Nov 15;4(6):753–760. doi: 10.1002/ijc.2910040602. [DOI] [PubMed] [Google Scholar]
  11. Baldwin R. W., Moore M. Isolation of membrane-associated tumour-specific antigens from rat hepatomas induced by aminoazo dye. Nature. 1968 Oct 19;220(5164):287–289. doi: 10.1038/220287a0. [DOI] [PubMed] [Google Scholar]
  12. Baldwin R. W., Moore M. Rat hepatoma cell surface antigens: demonstration and isolation of membrane-associated isoantigens. Eur J Cancer. 1969 Nov;5(5):475–483. doi: 10.1016/0014-2964(69)90101-7. [DOI] [PubMed] [Google Scholar]
  13. Baldwin R. W., Price M. R., Robins R. A. Blocking of lymphocyte-mediated cytotoxicity for rat hepatoma cells by tumour-specific antigen-antibody complexes. Nat New Biol. 1972 Aug 9;238(84):185–186. doi: 10.1038/newbio238185a0. [DOI] [PubMed] [Google Scholar]
  14. Baldwin R. W., Price M. R., Robins R. A. Inhibition of hepatoma-immune lymph-node cell cytotoxicity by tumour-bearer serum, and solubilized hepatoma antigen. Int J Cancer. 1973 May;11(3):527–535. doi: 10.1002/ijc.2910110304. [DOI] [PubMed] [Google Scholar]
  15. Bansal S. C., Sjögren H. O. Effects of BCG on various facets of the immune response against polyoma tumors in rats. Int J Cancer. 1973 Jan 15;11(1):162–171. doi: 10.1002/ijc.2910110119. [DOI] [PubMed] [Google Scholar]
  16. Holmes E. C., Kahan B. D., Morton D. L. Soluble tumor-specific transplantation antigens from methylcholanthrene-induced guinea pig sarcomas. Cancer. 1970 Feb;25(2):373–379. doi: 10.1002/1097-0142(197002)25:2<373::aid-cncr2820250215>3.0.co;2-l. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Meltzer M. S., Leonard E. J., Rapp H. J., Borsos T. Tumor-specific antigen solubilized by hypertonic potassium chloride. J Natl Cancer Inst. 1971 Sep;47(3):703–709. [PubMed] [Google Scholar]
  19. Oettgen H. F., Old L. J., McLean E. P., Carswell E. A. Delayed hypersensitivity and transplantation immunity elicited by soluble antigens of chemically induced tumours in inbred guinea-pigs. Nature. 1968 Oct 19;220(5164):295–297. doi: 10.1038/220295a0. [DOI] [PubMed] [Google Scholar]
  20. Ozer J. H., Wallace D. F. H-2 components and cellular membranes: distinctions between plasma membrane and endoplasmic reticulum governed by the H-2 region in the mouse. Transplantation. 1967 Jul;5(4):652–667. [PubMed] [Google Scholar]
  21. Suter L., Bloom B. R., Wadsworth E. M., Oettgen H. F. Use of the macrophage migration inhibition test to monitor fractionation of soluble antigens of chemically induced sarcomas of inbred guinea pigs. J Immunol. 1972 Oct;109(4):766–775. [PubMed] [Google Scholar]
  22. Thomson D. M., Alexander P. A cross-reacting embryonic antigen in the membrane of rat sarcoma cells which is immunogenic in the syngeneic host. Br J Cancer. 1973 Jan;27(1):35–47. doi: 10.1038/bjc.1973.5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Thomson D. M., Steele K., Alexander P. The presence of tumour-specific membrane antigen in the serum of rats with chemically induced sarcomata. Br J Cancer. 1973 Jan;27(1):27–34. doi: 10.1038/bjc.1973.4. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES