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. 1974 Jan;29(1):21–30. doi: 10.1038/bjc.1974.3

Antigens of Tumours Induced by Naturally Occurring Murine Sarcoma Virus (MSV-FBJ)

I. Detection of Group and Type Specific Antigens by Complement Fixation

D B Jones, M Moore
PMCID: PMC2009148  PMID: 4820943

Abstract

Antigens associated with cells transformed in vivo by FBJ virus, a wild type murine sarcoma virus (MSV) complex originating from a spontaneously arising osteosarcoma in a CF1 mouse, have been partially characterized by complement fixation (CF). Using rat antisera against antigens specified by Gross leukaemia virus (GLV) the group specific (gs) antigen of C-type RNA murine tumour viruses was demonstrated in FBJ tumours as well as in GLV rat leukaemias, AKR lymphomata and sarcomata induced by MSV-H (Harvey), an MSV isolate of Friend-Moloney-Rauscher (FMR) subgroup specificity. Using mouse antisera against antigens present in FBJ cells the Gross (G) or wild type specificity of FBJ tumours was demonstrated by cross reactivity with antigens expressed on normal AKR lymphoid tissues and leukaemias. These antigens were absent from MSV-H induced sarcomata and in reciprocal tests mouse antisera to MSV-H failed to react with antigens present in FBJ tumour cells. No distinction between cellular and virion antigens expressed by FBJ cells was possible by CF although evidence for a cellular antigen with G specificity was obtained in tests using aged C57B1 antiserum containing a naturally occurring G antibody lacking significant virus neutralizing capacity. However, the likelihood that mouse FBJ antisera contain antibodies to type specific viral envelope antigens (VEA) as well as cellular antigen is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoki T., Boyse E. A., Old L. J., De Harven E., Hämmerling U., Wood H. A. G (Gross) and H-2 cell-surface antigens: location on Gross leukemia cells by electron microscopy with visually labeled antibody. Proc Natl Acad Sci U S A. 1970 Mar;65(3):569–576. doi: 10.1073/pnas.65.3.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aoki T., Boyse E. A., Old L. J. Occurrence of natural antibody to the G (gross) leukemia antigen in mice. Cancer Res. 1966 Jul;26(7):1415–1419. [PubMed] [Google Scholar]
  3. Aoki T., Herberman R. B., Johnson P. A., Liu M., Sturm M. M. Wild-type gross leukemia virus: classification of soluble antigens (GSA). J Virol. 1972 Dec;10(6):1208–1219. doi: 10.1128/jvi.10.6.1208-1219.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chuat J. C., Berman L., Gunvén P., Klein E. Studies on Murine Sarcoma Virus: antigenic characterization of Murine Sarcoma Virus induced tumor cells. Int J Cancer. 1969 Jul 15;4(4):465–479. doi: 10.1002/ijc.2910040412. [DOI] [PubMed] [Google Scholar]
  5. Finkel M. P., Biskis B. O., Jinkins P. B. Virus induction of osteosarcomas in mice. Science. 1966 Feb 11;151(3711):698–701. doi: 10.1126/science.151.3711.698. [DOI] [PubMed] [Google Scholar]
  6. Geering G., Old L. J., Boyse E. A. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses. J Exp Med. 1966 Oct 1;124(4):753–772. doi: 10.1084/jem.124.4.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gilden R. V., Oroszlan S. Structural and immunologic relationships among mammalian C-type viruses. J Am Vet Med Assoc. 1971 Mar 15;158(6 Suppl):1099+–1099+. [PubMed] [Google Scholar]
  8. HARVEY J. J. AN UNIDENTIFIED VIRUS WHICH CAUSES THE RAPID PRODUCTION OF TUMOURS IN MICE. Nature. 1964 Dec 12;204:1104–1105. doi: 10.1038/2041104b0. [DOI] [PubMed] [Google Scholar]
  9. Hartley J. W., Rowe W. P., Capps W. I., Huebner R. J. Complement fixation and tissue culture assays for mouse leukemia viruses. Proc Natl Acad Sci U S A. 1965 May;53(5):931–938. doi: 10.1073/pnas.53.5.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Harvey J. J., East J. The murine sarcoma virus (MSV). Int Rev Exp Pathol. 1971;10:265–360. [PubMed] [Google Scholar]
  11. Herberman R. B. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J Natl Cancer Inst. 1972 Jan;48(1):265–271. [PubMed] [Google Scholar]
  12. JENKINS V. K., UPTON A. C. CELL-FREE TRANSMISSION OF RADIOGENIC MYELOID LEUKEMIA IN THE MOUSE. Cancer Res. 1963 Dec;23:1748–1755. [PubMed] [Google Scholar]
  13. Jones D. B., Moore M. Tumour-associated transplantation antigens of neoplasms induced by a naturally occurring murine sarcoma virus (FBJ-MSV). Br J Cancer. 1973 Jun;27(6):415–426. doi: 10.1038/bjc.1973.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kelloff G. J., Lane W. T., Turner H. C., Huebner R. J. In vivo studies of the FBJ murine osteosarcoma virus. Nature. 1969 Sep 27;223(5213):1379–1380. doi: 10.1038/2231379a0. [DOI] [PubMed] [Google Scholar]
  15. Lejneva O. M., Abelev G. I. Immunofluorescent study of the group-specific antigen of murine leukemia viruses. Int J Cancer. 1970 Sep 15;6(2):153–159. doi: 10.1002/ijc.2910060203. [DOI] [PubMed] [Google Scholar]
  16. Levy J. A., Hartley J. W., Rowe W. P., Huebner R. J. Studies of FBJ osteosarcoma virus in tissue culture. I. Biologic characteristics of the "C"-type viruses. J Natl Cancer Inst. 1973 Aug;51(2):525–539. [PubMed] [Google Scholar]
  17. Moore M., Williams D. E. Studies on the antigenicity of radiation-induced murine osteosarcomata. Br J Cancer. 1972 Apr;26(2):90–98. doi: 10.1038/bjc.1972.14. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Price C. H., Moore M., Jones D. B. FBJ virus-induced tumours in mice. A histopathological study of FBJ virus tumours and their relevance to murine and human osteosarcoma arising in bone. Br J Cancer. 1972 Feb;26(1):15–27. doi: 10.1038/bjc.1972.5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rhim J. S., Huebner R. J., Lane W. T., Turner H. C., Rabstein L. Neoplastic transformation and derivation of a focus-forming sarcoma virus in cultures of rat embryo cells infected with a murine osteosarcoma (FBJ) virus. Proc Soc Exp Biol Med. 1969 Dec;132(3):1091–1098. doi: 10.3181/00379727-132-34373. [DOI] [PubMed] [Google Scholar]
  20. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  21. Schäfer W., Anderer F. A., Bauer H., Pister L. Studies on mouse leukemia viruses. I. Isolation and characterization of a group-specific antigen. Virology. 1969 Jul;38(3):387–394. doi: 10.1016/0042-6822(69)90151-2. [DOI] [PubMed] [Google Scholar]
  22. Stockert E., Old L. J., Boyse E. A. The G-IX system. A cell surface allo-antigen associated with murine leukemia virus; implications regarding chromosomal integration of the viral genome. J Exp Med. 1971 Jun 1;133(6):1334–1355. doi: 10.1084/jem.133.6.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yumoto T., Poel W. E., Kodama T., Dmochowski L. Studies on FBJ virus-induced bone tumors in mice. Tex Rep Biol Med. 1970 Spring;28(1):145–165. [PubMed] [Google Scholar]

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