Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1974 Nov;30(5):394–400. doi: 10.1038/bjc.1974.213

Immunogenic Properties of Rat Hepatoma Subcellular Fractions

M R Price, R W Baldwin
PMCID: PMC2009306  PMID: 4377707

Abstract

Subcellular fractions from an aminoazo dye induced rat hepatoma (D23) were examined for their ability to evoke rejection responses in syngeneic hosts to transplanted tumour cells and to induce the production of humoral antibody. Membrane fractions isolated by zonal centrifugation and displaying an increased activity of tumour specific antigen (Price and Baldwin, 1974), as well as crude membrane fractions and purified tumour cell ghosts, all elicited tumour specific antibody demonstrable by membrane immunofluorescence staining of viable hepatoma D23 cells. Tumour cell nuclei or soluble cytoplasmic protein were, however, lacking in this capacity. Resistance to tumour cell challenge was not observed in rats treated with any of the hepatoma D23 subcellular fractions administered by various routes either alone or in admixture with bacterial adjuvants. These findings are relevant to current views that tumour immunity may be more optimally achieved by inoculation of intact (viable or attenuated) tumour cells.

Full text

PDF
394

Images in this article

397Fig.
on p.397

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baldwin R. W., Barker C. R. Demonstration of tumour-specific humoral antibody against aminoazo dye-induced rat hepatomata. Br J Cancer. 1967 Dec;21(4):793–800. doi: 10.1038/bjc.1967.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baldwin R. W., Bowen J. G., Price M. R. Detection of circulating hepatoma D23 antigen and immune complexes in tumour bearer serum. Br J Cancer. 1973 Jul;28(1):16–24. doi: 10.1038/bjc.1973.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baldwin R. W., Embleton M. J., Moore M. Immunogenicity of rat hepatoma membrane fractions. Br J Cancer. 1973 Nov;28(5):389–399. doi: 10.1038/bjc.1973.164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baldwin R. W., Glaves D. Solubilization of tumour-specific antigen from plasma membrane of an aminoazo-dye-induced rat hepatoma. Clin Exp Immunol. 1972 May;11(1):51–56. [PMC free article] [PubMed] [Google Scholar]
  5. Baldwin R. W., Moore M. Isolation of membrane-associated tumour-specific antigen from an aminoazo-dye-induced rat hepatoma. Int J Cancer. 1969 Nov 15;4(6):753–760. doi: 10.1002/ijc.2910040602. [DOI] [PubMed] [Google Scholar]
  6. Baldwin R. W., Pimm M. V. BCG immunotherapy of a rat sarcoma. Br J Cancer. 1973 Oct;28(4):281–287. doi: 10.1038/bjc.1973.149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holmes E. C., Kahan B. D., Morton D. L. Soluble tumor-specific transplantation antigens from methylcholanthrene-induced guinea pig sarcomas. Cancer. 1970 Feb;25(2):373–379. doi: 10.1002/1097-0142(197002)25:2<373::aid-cncr2820250215>3.0.co;2-l. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. McCollester D. L. Isolation of meth A cell surface membranes possessing tumor-specific transplantation antigen activity. Cancer Res. 1970 Dec;30(12):2832–2837. [PubMed] [Google Scholar]
  10. Meltzer M. S., Oppenheim J. J., Littman B. H., Leonard E. J., Rapp H. J. Cell-mediated tumor immunity measured in vitro and in vivo with soluble tumor-specific antigens. J Natl Cancer Inst. 1972 Sep;49(3):727–734. [PubMed] [Google Scholar]
  11. Oettgen H. F., Old L. J., McLean E. P., Carswell E. A. Delayed hypersensitivity and transplantation immunity elicited by soluble antigens of chemically induced tumours in inbred guinea-pigs. Nature. 1968 Oct 19;220(5164):295–297. doi: 10.1038/220295a0. [DOI] [PubMed] [Google Scholar]
  12. PREHN R. T., MAIN J. M. Immunity to methylcholanthrene-induced sarcomas. J Natl Cancer Inst. 1957 Jun;18(6):769–778. [PubMed] [Google Scholar]
  13. Parish C. R. Immune response to chemically modified flagellin. I. Induction of antibody tolerance to flagellin by acetoacetylated derivatives of the protein. J Exp Med. 1971 Jul 1;134(1):1–20. doi: 10.1084/jem.134.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Parish C. R. Immune response to chemically modified flagellin. II. Evidence for a fundamental relationship between humoral and cell-mediated immunity. J Exp Med. 1971 Jul 1;134(1):21–47. doi: 10.1084/jem.134.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pilch Y. H. The antigenicity and immunogenicity of cell-free extracts of chemically induced murine sarcomas. Cancer Res. 1968 Dec;28(12):2502–2511. [PubMed] [Google Scholar]
  16. Price M. R., Baldwin R. W. Preparation of aminoazo dye induced rat hepatoma membrane fractions retaining tumour specific antigen. Br J Cancer. 1974 Nov;30(5):382–393. doi: 10.1038/bjc.1974.212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ranney D. F., Gordon R. O., Pincus J. H., Oppenheim J. J. Biological effects of murine histocompatibility antigen solubilized with 3 M potassium chloride. Transplantation. 1973 Dec;16(6):558–564. doi: 10.1097/00007890-197312000-00005. [DOI] [PubMed] [Google Scholar]
  18. Rosenberg E. B., Hill J., Ferarri A., Herberman R. B., Ting C. C., Mann D. L., Fahey J. Prolonged survival of tumor allografts in mice pretreated with soluble transplantation antigens. J Natl Cancer Inst. 1973 Jun;50(6):1453–1458. doi: 10.1093/jnci/50.6.1453. [DOI] [PubMed] [Google Scholar]
  19. Schirrmacher V., Wigzell H. Immune responses against native and chemically modified albumins in mice. I. Analysis of non-thymus-processed (B) and thymus-processed (T) cell responses against methylated bovine serum albumin. J Exp Med. 1972 Dec 1;136(6):1616–1630. doi: 10.1084/jem.136.6.1616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sumerska T., Betel I., Balner H., Warren H. S. Prolongation of skin allograft survival with antilymphocyte serum and soluble histocompatibility antigens. Transplantation. 1974 Jan 1;17(1):1–7. doi: 10.1097/00007890-197401000-00001. [DOI] [PubMed] [Google Scholar]
  21. Wagner H. Cell-mediated immune response in vitro. IV. Metabolic studies on cellular immunogenicity. Eur J Immunol. 1973 Feb;3(2):84–89. doi: 10.1002/eji.1830030206. [DOI] [PubMed] [Google Scholar]
  22. Wolf A., Avis P. J. Preparation and purification of plasma membranes from murine lymphoma cells carrying tumour-specific antigenicity. Transplantation. 1970 Jan;9(1):18–29. doi: 10.1097/00007890-197001000-00003. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES