Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1975 Feb;31(2):207–217. doi: 10.1038/bjc.1975.27

An electron microscopic study of tumour cell adhesiveness induced by aggregation promoting factor from rat ascites hepatoma cells.

Y Ishimaru, H Ishihara, H Hayashi
PMCID: PMC2009402  PMID: 169862

Abstract

A substance capable of inducing tumour cell aggregation, which is supposed to be a glycoprotein showing noncytotoxicity, was separated from rat ascites hepatoma cells and partially purified by chromatography. Adhesiveness of rat ascites hepatoma cells induced by this substance was characterized by gradual development of known binding structures during a period of 24 h after contact with the substance; simple apposition and intermediate junctions developed in the early stage, and desmosomes and focal tight junctions in the later stage. It was assumed that the substance might be involved in the development of such binding structures as a triggering mechanism of tumour cell adhesiveness.

Full text

PDF
207

Images in this article

210Fig. 2
on p.210
210Fig. 3
on p.210
210Fig. 4
on p.210
212Fig. 5
on p.212
212Fig. 6
on p.212
212Fig. 7
on p.212
212Fig. 8
on p.212
209Fig. 1a
on p.209
209Fig. 1b
on p.209
213Fig. 9
on p.213
214Fig. 10
on p.214
214Fig. 11
on p.214
214Fig. 12
on p.214

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABERCROMBIE M., AMBROSE E. J. The surface properties of cancer cells: a review. Cancer Res. 1962 Jun;22:525–548. [PubMed] [Google Scholar]
  2. Adler R. Ultrastructural changes associated with an invagination phenomenon in embryonic neural aggregates. Exp Cell Res. 1971 Oct;68(2):395–403. doi: 10.1016/0014-4827(71)90165-0. [DOI] [PubMed] [Google Scholar]
  3. Armstrong P. B. A fine structural study of adhesive cell junctions in heterotypic cell aggregates. J Cell Biol. 1970 Oct;47(1):197–210. doi: 10.1083/jcb.47.1.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Biberfeld P. Uropod formation in phytohaemagglutinin (PHA) stimulated lymphocytes. Exp Cell Res. 1971 Jun;66(2):433–445. doi: 10.1016/0014-4827(71)90698-7. [DOI] [PubMed] [Google Scholar]
  5. Burger M. M. A difference in the architecture of the surface membrane of normal and virally transformed cells. Proc Natl Acad Sci U S A. 1969 Mar;62(3):994–1001. doi: 10.1073/pnas.62.3.994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FARQUHAR M. G., PALADE G. E. Junctional complexes in various epithelia. J Cell Biol. 1963 May;17:375–412. doi: 10.1083/jcb.17.2.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gould R. P., Day A., Wolpert L. Mesenchymal condensation and cell contact in early morphogenesis of the chick limb. Exp Cell Res. 1972 May;72(1):325–336. doi: 10.1016/0014-4827(72)90593-9. [DOI] [PubMed] [Google Scholar]
  8. Heaysman J. E., Pegrum S. M. Early contacts between fibroblasts. An ultrastructural study. Exp Cell Res. 1973 Mar 30;78(1):71–78. doi: 10.1016/0014-4827(73)90039-6. [DOI] [PubMed] [Google Scholar]
  9. Jones D. B. The morphology of acid mucosubstances in leukocytic sticking to endothelium in acute inflammation. Lab Invest. 1970 Dec;23(6):606–611. [PubMed] [Google Scholar]
  10. Koono M., Katsuya H., Hayashi H. Studies on the mechanisms of invasion in cancer. IV. A factor associated with release of neutral protease of tumor cell. Int J Cancer. 1974 Mar 15;13(3):334–342. doi: 10.1002/ijc.2910130309. [DOI] [PubMed] [Google Scholar]
  11. Koono M., Ushijima K., Hayashi H. Studies on the mechanisms of invasion in cancer. 3. Purification of a neutral protease of rat ascites hepatoma cell associated with production of chemotactic factor for cancer cells. Int J Cancer. 1974 Jan 15;13(1):105–115. doi: 10.1002/ijc.2910130112. [DOI] [PubMed] [Google Scholar]
  12. Kudo K., Tasaki I., Hanaoka Y., Hayashi H. A tumour cell aggregation promoting substance from rat ascites hepatoma cells. Br J Cancer. 1974 Dec;30(6):549–559. doi: 10.1038/bjc.1974.234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lentz T. L., Trinkaus J. P. Differentiation of the junctional complex of surface cells in the developing Fundulus blastoderm. J Cell Biol. 1971 Mar;48(3):455–472. doi: 10.1083/jcb.48.3.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lilien J. E., Moscona A. A. Cell aggregation: its enhancement by a supernatant from cultures of homologous cells. Science. 1967 Jul 7;157(3784):70–72. doi: 10.1126/science.157.3784.70. [DOI] [PubMed] [Google Scholar]
  15. MOSCONA A. Rotation-mediated histogenetic aggregation of dissociated cells. A quantifiable approach to cell interactions in vitro. Exp Cell Res. 1961 Jan;22:455–475. doi: 10.1016/0014-4827(61)90122-7. [DOI] [PubMed] [Google Scholar]
  16. Martínez-Palomo A. Ultrastructural modifications of intercellular junctions in some epithelial tumors. Lab Invest. 1970 Jun;22(6):605–614. [PubMed] [Google Scholar]
  17. Millonig G., Giudice G. Electron microscopic study of the reaggregation of cells dissociated from sea urchin embryos. Dev Biol. 1967 Feb;15(2):91–101. doi: 10.1016/0012-1606(67)90008-5. [DOI] [PubMed] [Google Scholar]
  18. ODASHIMA S. Comparative studies on the transplantability of liver cancers induced in rats fed with 3'-methyl-4-dimethylaminoazobenzene for 3-6 months. Gan. 1962 Dec;53:325–348. [PubMed] [Google Scholar]
  19. ODASHIMA S. ESTABLISHMENT OF ASCITES HEPATOMAS IN THE RAT, 1951-1962. Natl Cancer Inst Monogr. 1964 Dec;16:51–93. [PubMed] [Google Scholar]
  20. Ogata T. The role of inflammatory chemotactic factor (leukoegresin) and permeability factor (vasoexin) in acute inflammation: an electron microscopic observation of biologic action of these natural mediators. Kumamoto Med J. 1971 Dec 31;24(3):103–123. [PubMed] [Google Scholar]
  21. Trelstad R. L., Hay E. D., Revel J. D. Cell contact during early morphogenesis in the chick embryo. Dev Biol. 1967 Jul;16(1):78–106. doi: 10.1016/0012-1606(67)90018-8. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES