Abstract
A single injection of urethane into adult male A2G mice produced an increase in the proliferative rate of alveolar wall cells, reaching a peak at 2 weeks post urethan (PU) and declining to control levels by 2 months PU. During this urethane induced proliferative response the single and double labelling indices and the native metaphase index were all elevated although there was no corresponding alteration in the arrested metaphase index. This proliferative response may not be restricted to hyperplasia of potentially neoplastic cells, such as type II epithelium, but may also include type I epithelial cells and alveolar macrophage precursors. However, it was impossible to identify individual cell populations by methods used. The growth rate of adenomata decrease with time and cell kinetic techniques showed that the rates of entry of adenoma cells into DNA synthesis and into metaphase were decreasing concurrently with the growth rate. Thus the rate of cell production falls as adenomata age but how much cell loss contributes to the decrease in growth rate is not yet known. Decreasing cell production could be due to an increased cell cycle time and/or a decreased growth fraction. The duration of DNA synthesis in adenomata increased markedly as the mice survived, suggesting that the cell cycle time might be increased, but further experiments are required to determine whether the growth fraction changes. Attention is drawn to a complication that Colcemid introduces into kinetic studies on alveolar wall cells.
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- BERTALANFFY F. D., SCHACHTER R., ALI J., INGIMUNDSON J. C. MITOTIC RATE AND DOUBLING TIME OF INTRAPERITONEAL AND SUBCUTANEOUS EHRLICH ASCITES TUMOR. Cancer Res. 1965 Jun;25:685–691. [PubMed] [Google Scholar]
- Bertalanffy F. D. Respiratory tissue: structure, histophysiology, cytodynamics. II. New approaches and interpretations. Int Rev Cytol. 1964;17:213–297. doi: 10.1016/s0074-7696(08)60408-8. [DOI] [PubMed] [Google Scholar]
- Dixon W. E., Malden W. Colchicine with special reference to its mode of action and effect on bone-marrow. J Physiol. 1908 May 6;37(1):50–76. doi: 10.1113/jphysiol.1908.sp001257. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FOLEY W. A., COLE L. J., INGRAM B. J., CROCKER T. T. X-RAY INHIBITION OF URETHAN-STIMULATED PROLIFERATION OF LUNG CELLS OF THE MOUSE AS ESTIMATED BY INCORPORATION OF TRITIATED THYMIDINE. Nature. 1963 Sep 28;199:1267–1268. doi: 10.1038/1991267a0. [DOI] [PubMed] [Google Scholar]
- FREI E., 3rd, WHANG J., SCOGGINS R. B., VANSCOTT E. J., RALL D. P., BEN M. THE STATHMOKINETIC EFFECT OF VINCRISTIME. Cancer Res. 1964 Dec;24:1918–1925. [PubMed] [Google Scholar]
- Kauffman S. L. Alterations in cell proliferation in mouse lung following urethane exposure. I. The nonvaculoated alveolar cell. Am J Pathol. 1969 Jan;54(1):83–93. [PMC free article] [PubMed] [Google Scholar]
- Kauffman S. L. Kinetics of alveolar epithelial hyperplasia in lungs of mice exposed to urethane. I. Quantitative analysis of cell populations. Lab Invest. 1974 Feb;30(2):170–175. [PubMed] [Google Scholar]
- PUCK T. T., STEFFEN J. LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. I. A METHOD FOR LOCALIZING METABOLIC EVENTS WITHIN THE LIFE CYCLE, AND ITS APPLICATION TO THE ACTION OF COLCEMIDE AND SUBLETHAL DOSES OF X-IRRADIATION. Biophys J. 1963 Sep;3:379–397. doi: 10.1016/s0006-3495(63)86828-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROGERS S. Age of the host and other factors affecting the production with urethane of pulmonary adenomas in mice. J Exp Med. 1951 May;93(5):427–449. doi: 10.1084/jem.93.5.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shimkin M. B., Sasaki T., McDonough M., Baserga R., Thatcher D., Wieder R. Relation of thymidine index to pulmonary tumor response in mice receiving urethan and other carcinogens. Cancer Res. 1969 May;29(5):994–998. [PubMed] [Google Scholar]