Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1975 Apr;31(4):424–428. doi: 10.1038/bjc.1975.81

High affinity oestradiol receptors and the activity of glucose-6-phosphate dehydrogenase and lactose synthetase in mammary carcinomata of postmenopausal women.

J L Daehnfeldt, M Schülein
PMCID: PMC2009451  PMID: 168914

Abstract

The determination of hormone inducible proteins in endocrine tumours may yield information about the presence of hormone dependent tumour cells. We have estimated the high affinity oestradiol binding capacity in primary mammary carcinomata of 57 postmenopausal patients. Glucose-6-phosphate dehydrogenase and lactose synthetase are known from animal experiments to be hormone inducible. Therefore, in biopsies of sufficient size the activity of glucose-6-phosphate dehydrogenase (47 pateints) and lactose synthetase (23 patients) was also studied. It was found that biopsies with high binding capacity also showed high activities of glucose-6-phosphate dehydrogenase and lactose synthetase A protein (galactosyl transferase). No lactose synthetase B protein (alpha-lactalbumin) has been discovered in the tumours. The present observations may be considered suggestive evidence of a relationship between high oestradiol binding capcity and high activities of the two enzymes on the one hand and hormone dependence of the tumour on the other. However, further clinical studies are required before final conclusions in this respect can be drawn.

Full text

PDF
424

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brew K., Vanaman T. C., Hill R. L. The role of alpha-lactalbumin and the A protein in lactose synthetase: a unique mechanism for the control of a biological reaction. Proc Natl Acad Sci U S A. 1968 Feb;59(2):491–497. doi: 10.1073/pnas.59.2.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Briand P., Daehnfeldt J. L. Enzyme patterns of glucose catabolism in hormone-dependent and -independent mammary tumours of GR mice. Eur J Cancer. 1973 Oct;9(10):763–770. doi: 10.1016/0014-2964(73)90069-8. [DOI] [PubMed] [Google Scholar]
  3. Daehnfeldt J. L. Endogenously blocked high affinity estradiol receptors in the immature and mature rat uterus. Proc Soc Exp Biol Med. 1974 May;146(1):159–162. doi: 10.3181/00379727-146-38062. [DOI] [PubMed] [Google Scholar]
  4. Engelsman E., Persijn J. P., Korsten C. B., Cleton F. J. Oestrogen receptor in human breast cancer tissue and response to endocrine therapy. Br Med J. 1973 Jun 30;2(5869):750–752. doi: 10.1136/bmj.2.5869.750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. England P. C., Skinner L. G., Cottrell K. M., Sellwood R. A. Serum oestradiol-17 beta in normal women. Br J Cancer. 1974 Jun;29(6):462–469. doi: 10.1038/bjc.1974.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GLOCK G. E., McLEAN P. Further studies on the properties and assay of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver. Biochem J. 1953 Oct;55(3):400–408. doi: 10.1042/bj0550400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hilf R., Wittliff J. L., Rector W. D., Savlov E. D., Hall T. C., Orlando R. A. Studies on certain cytoplasmic enzymes and specific estrogen receptors in human breast cancer and in nonmalignant diseases of the breast. Cancer Res. 1973 Sep;33(9):2054–2062. [PubMed] [Google Scholar]
  8. Leclercq G., Heuson J. C., Schoenfeld R., Mattheiem W. H., Tagnon H. J. Estrogen receptors in human breast cancer. Eur J Cancer. 1973 Sep;9(9):665–673. doi: 10.1016/0014-2964(73)90009-1. [DOI] [PubMed] [Google Scholar]
  9. Méster J., Rbertson D. M., Feherty P., Kellie A. E. Determination of high-affinity oestrogen receptor sites in uterine supernatant preparations. Biochem J. 1970 Dec;120(4):831–836. doi: 10.1042/bj1200831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Palmiter R. D. Hormonal induction and regulation of lactose synthetase in mouse mammary gland. Biochem J. 1969 Jun;113(2):409–417. doi: 10.1042/bj1130409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schülein M., Daehnfeldt J. L., Briand P. Lactose synthetase induction by hormones in normal and tumorous GR mouse mammary tissue. Int J Cancer. 1974 Sep 15;14(3):372–378. doi: 10.1002/ijc.2910140311. [DOI] [PubMed] [Google Scholar]
  12. Terenius L. Parallelism between oestrogen binding capacity and hormone responsiveness of mammary tumours in GR-A mice. Eur J Cancer. 1972 Feb;8(1):55–58. doi: 10.1016/0014-2964(72)90083-7. [DOI] [PubMed] [Google Scholar]
  13. Turkington R. W., Brew K., Vanaman T. C., Hill R. L. The hormonal control of lactose synthetase in the developing mouse mammary gland. J Biol Chem. 1968 Jun 25;243(12):3382–3387. [PubMed] [Google Scholar]
  14. Wagner R. K. Characterization and assay of steroid hormone receptors and steroid-binding serum proteins by agargel electrophoresis at low temperature. Hoppe Seylers Z Physiol Chem. 1972 Aug;353(8):1235–1245. doi: 10.1515/bchm2.1972.353.2.1235. [DOI] [PubMed] [Google Scholar]
  15. Wittliff J. L., Hilf R., Brooks W. F., Jr, Savlov E. D., Hall T. C., Orlando R. A. Specific estrogen-binding capacity of the cytoplasmic receptor in normal and neoplastic breast tissues of humans. Cancer Res. 1972 Sep;32(9):1983–1992. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES