Abstract
Saline extracts of human bronchogenic tumours, soluble in 50% saturated ammonium sulphate and also fractions from Sephadex G-200 chromatography were used to raise antisera in rabbits. After absorbing the antisera with normal tissue extracts, direct Ouchterlony tests were performed against tumour (adenocarcinomata and squamous cell carcinomata) and normal extracts. A precipitin reaction was given with all 11 tumour extracts tested at a concentration of 5 mg/ml whereas all the 9 normal lung control extracts did not react at concentrations up to 100 mg/ml. The possibility that this reaction could be related to histocompatibility differences between individuals is ruled out by the fact that in two cases tumour and normal tissue were obtained from the same patient. These studies and also precipitin-inhibition experiments have confirmed the existence of antigen associated with bronchial carcinomata and have shown that, although the antigen or a cross-reacting antigen is present in normal lung tissue, the amounts are small in comparison with the amounts extracted from tumour. Antigenic activity was contained in a single absorbance peak when fractionated by Sephadex G-200 chromatography and its elution volume indicated a molecular weight of approximately 4-0 times 10(4)D. Further purification was achieved using isotachophoresis. Preliminary characterization of the antigen has shown it to be stable at pH 4-5, resistant to heating at 50 degrees C for 30 min, to migrate on immunoelectrophoresis with a cationic mobility at PH 8-5 and to be immunologically distinct from carcinoembryonic antigen.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alexander P. Foetal "antigens" in cancer. Nature. 1972 Jan 21;235(5334):137–140. doi: 10.1038/235137a0. [DOI] [PubMed] [Google Scholar]
- Avis P., Lewis M. G. Brief communication: tumor-associated fetal antigens in human tumors. J Natl Cancer Inst. 1973 Sep;51(3):1063–1066. doi: 10.1093/jnci/51.3.1063. [DOI] [PubMed] [Google Scholar]
- Cinader B. The future of tumor immunology. Med Clin North Am. 1972 May;56(3):801–836. doi: 10.1016/s0025-7125(16)32394-x. [DOI] [PubMed] [Google Scholar]
- GOLD P., FREEDMAN S. O. DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUES. J Exp Med. 1965 Mar 1;121:439–462. doi: 10.1084/jem.121.3.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Khoo S. K., Warner N. L., Lie J. T., Mackay I. R. Carcinoembryonic antigenic activity of tissue extracts: a quantitative study of malignant and benign neoplasms, cirrhotic liver, normal adult and fetal organs. Int J Cancer. 1973 May;11(3):681–687. doi: 10.1002/ijc.2910110319. [DOI] [PubMed] [Google Scholar]
- Louis C. J., Blunck J. M., Richmond L. M. Agarose-gel electrophoresis of soluble proteins from bronchial mucosa and bronchogenic carcinoma. Oncology. 1973;27(4):324–335. doi: 10.1159/000224745. [DOI] [PubMed] [Google Scholar]
- ROSE N. R., SHULMAN S., WITEBSKY E. Studies of normal and malignant tissue antigens. Cancer Res. 1956 Oct;16(9):831–841. [PubMed] [Google Scholar]
- TATARINOV Iu S. OBNARUZHENIE EMBRIOSPETSIFICHESKOGO ALPHA-GLOBULINA V SYVOROTKE KROVI BOL'NOGO PERVICHNYM RAKOM PECHENI. Vopr Med Khim. 1964 Jan-Feb;10:90–91. [PubMed] [Google Scholar]
- Watson R. D., Smith A. G., Levy J. G. The use of immunoadsorbent columns for the isolation of antibodies specific for antigens associated with human bronchogenic carcinoma. Br J Cancer. 1974 Mar;29(3):183–188. doi: 10.1038/bjc.1974.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yachi A., Matsuura Y., Carpenter C. M., Hyde L. Immunochemical studies on human lung cancer antigens soluble in 50 per cent saturated ammonium sulfate. J Natl Cancer Inst. 1968 Apr;40(4):663–682. [PubMed] [Google Scholar]