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. 1975 Jun;31(6):641–648. doi: 10.1038/bjc.1975.110

Inhibition of normal human in vitro colony forming cells by cells from leukaemic patients.

T C Morris, T A McNeill, J M Bridges
PMCID: PMC2009482  PMID: 1058036

Abstract

Co-culture in agar of normal bone marrow cells from different individuals gave granulocyte macrophage colony counts that were expected from counts made when the marrows were cultured separately. Co-culture of normal marrow with normal peripheral blood leucocytes (which did not themselves give rise to colonies) caused inhibition of colony growth only when the ratio of peripheral blood to bone marrow cells was of the order of 4 : 1. Peripheral blood or bone marrow cells from 7 of 9 patients with acute myelomonocytic leukaemia, which did not give rise to colonies, caused a marked reduction in the number of colonies obtained from normal marrow cells when cultured with them. This inhibitory effect of leukaemic cells was found when ratios of leukaemic to normal cells were as low as 1 : 4. Additional evidence that the inhibition of normal colony formation was related to the leukaemic process was obtained from follow-up studies on one of the patients whose cells lost the capacity to inhibit normal colony formation during remission and became inhibitory again on relapse.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradley T. R., Metcalf D. The growth of mouse bone marrow cells in vitro. Aust J Exp Biol Med Sci. 1966 Jun;44(3):287–299. doi: 10.1038/icb.1966.28. [DOI] [PubMed] [Google Scholar]
  2. Bradley T. R., Sumner M. A. Stimulation of mouse bone marrow colony growth in vitro by conditioned medium. Aust J Exp Biol Med Sci. 1968 Oct;46(5):607–618. doi: 10.1038/icb.1968.167. [DOI] [PubMed] [Google Scholar]
  3. Bull J. M., Duttera M. J., Stashick E. D., Northup J., Henderson E., Carbone P. P. Serial in vitro marrow culture in acute myelocytic leukemia. Blood. 1973 Nov;42(5):679–686. [PubMed] [Google Scholar]
  4. Greenberg P. L., Nichols W. C., Schrier S. L. Granulopoiesis in acute myeloid leukemia and preleukemia. N Engl J Med. 1971 Jun 3;284(22):1225–1232. doi: 10.1056/NEJM197106032842201. [DOI] [PubMed] [Google Scholar]
  5. Haskill J. S., McKnight R. D., Galbraith P. R. Cell-cell interaction in vitro: studied by density separation of colony-forming, stimulating, and inhibiting cells from human bone marrow. Blood. 1972 Sep;40(3):394–399. [PubMed] [Google Scholar]
  6. Hulliger L., Blazkovec A. A. A simple and efficient method of separating peripheral-blood leucocytes for in-vitro studies. Lancet. 1967 Jun 17;1(7503):1304–1305. doi: 10.1016/s0140-6736(67)91596-6. [DOI] [PubMed] [Google Scholar]
  7. Kurnick J. E., Robison W. A. Clony growth of human peripherl white blood cells in vitro. Blood. 1971 Feb;37(2):136–141. [PubMed] [Google Scholar]
  8. McNeill T. A. The effect of synthetic double-stranded polyribonucleotides on haemopoietic colony-forming cells in vitro. Immunology. 1971 Nov;21(5):741–750. [PMC free article] [PubMed] [Google Scholar]
  9. Messner H. A., McCulloch E. A. Interacting cell populations affecting granulopoietic colony formation by normal and leukemic human marrow cells. Blood. 1973 Nov;42(5):701–710. [PubMed] [Google Scholar]
  10. Metcalf D. Regulation of granulocyte and monocyte-macrophage proliferation by colony stimulating factor (CSF): a review. Exp Hematol. 1973;1(4):185–201. [PubMed] [Google Scholar]
  11. Moore M. A., Spitzer G., Williams N., Metcalf D., Buckley J. Agar culture studies in 127 cases of untreated acute leukemia: the prognostic value of reclassification of leukemia according to in vitro growth characteristics. Blood. 1974 Jul;44(1):1–18. [PubMed] [Google Scholar]
  12. Moore M. A., Williams N., Metcalf D. In vitro colony formation by normal and leukemic human hematopoietic cells: interaction between colony-forming and colony-stimulating cells. J Natl Cancer Inst. 1973 Mar;50(3):591–602. doi: 10.1093/jnci/50.3.591. [DOI] [PubMed] [Google Scholar]
  13. Paran M., Ichikawa Y., Sachs L. Feedback inhibition of the development of macrophage and granulocyte colonies. II. Inhibition by granulocytes. Proc Natl Acad Sci U S A. 1969 Jan;62(1):81–87. doi: 10.1073/pnas.62.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Petrov R. V., Seslavina L. S., Pantelejev E. I. Stem-cell inactivation in mixed spleen cell cultures. Nature. 1968 Feb 10;217(5128):558–560. doi: 10.1038/217558a0. [DOI] [PubMed] [Google Scholar]
  15. Robinson W. A., Kurnick J. E., Pike B. L. Colony growth of human leukemic peripheral blood cells in vitro. Blood. 1971 Oct;38(4):500–508. [PubMed] [Google Scholar]

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