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. 1978 May;37(5):797–805. doi: 10.1038/bjc.1978.118

Fluorescence-polarization changes in mononuclear blood leucocytes after PHA incubation: differences in cells from patients with and without neoplasia.

H Kreutzmann, T M Fliedner, H J Galla, E Sackmann
PMCID: PMC2009621  PMID: 656307

Abstract

In 32 healthy blood donors, 20 patients with histologically verified cancer and 18 patients with non-neoplastic diseases, the fluorescence polarization changes of fluorescein samples incorporated in mononuclear leucotyes were measured after incubation with PHA. The leucocytes of healthy persons, and 16/18 persons with non-neoplastic diseases, responded with a decrease in the degree of fluorescence polarization by about 20% from that in non-PHA-stimulated cells. In 19/20 patients with a variety of malignant tumours, the leucotyes did not respond to PHA stimulation with such a decrease. The exceptions among the patients with neoplastic and non-neoplastic diseases are considered, and may not be "false-negative" or "false-positive" respectively, but indicative of a particular situation in that disease. The biophysical mechanisms underlying the observed changes remain to be investigated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHEN R. F., BOWMAN R. L. FLUORESCENCE POLARIZATION: MEASUREMENT WITH ULTRAVIOLET-POLARIZING FILTERS IN A SPECTROPHOTOFLUOROMETER. Science. 1965 Feb 12;147(3659):729–732. doi: 10.1126/science.147.3659.729. [DOI] [PubMed] [Google Scholar]
  2. Cercek L., Cercek B. Application of the phenomenon of changes in the structuredness of cytoplasmic matrix (SCM) in the diagnosis of malignant disorders: a review. Eur J Cancer. 1977 Sep;13(9):903–915. doi: 10.1016/0014-2964(77)90166-9. [DOI] [PubMed] [Google Scholar]
  3. Cercek L., Cercek B. Changes in the SCM response ratio (RRscm) after surgical removal of malignant tissue. Br J Cancer. 1975 Feb;31(2):250–251. doi: 10.1038/bjc.1975.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cercek L., Cercek B. Changes in the structuredness of cytoplasmic matrix (SCM) in human lymphocytes induced by PHA and cancer basic protein as measured in single cells. Br J Cancer. 1976 May;33(5):539–543. doi: 10.1038/bjc.1976.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cercek L., Cercek B., Franklin C. I. Biophysical differentiation between lymphocytes from healthy donors, patients with malignant diseases and other disorders. Br J Cancer. 1974 May;29(5):345–352. doi: 10.1038/bjc.1974.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sterioff S., Rios C. N., Zachary J. B., Williams G. M. Neoplasia in kidney transplant recipients. Am J Surg. 1975 Nov;130(5):622–626. doi: 10.1016/0002-9610(75)90524-3. [DOI] [PubMed] [Google Scholar]
  8. Weedon D. D., Shorter R. G., Ilstrup D. M., Huizenga K. A., Taylor W. F. Crohn's disease and cancer. N Engl J Med. 1973 Nov 22;289(21):1099–1103. doi: 10.1056/NEJM197311222892101. [DOI] [PubMed] [Google Scholar]

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