Abstract
A fraction showing tumour-associated antigenic properties has been isolated from pleural effusions of patients with squamous-cell carcinoma of the lung. Purification of the material was accomplished by ion-exchange and affinity chromatography, and by immunoabsorbents. The antigenic activity was monitored by its inhibitory capacity in a specific complement-dependent cytotoxic system. The final fraction has a mol. wt. of approximately 1.7 X 10(5), as judged by gel filtration on Sephadex G200, and the main component appears to be a glycoprotein with N-acetyl-D-glucosamine groups. The most purified antigen preparation exhibited a highly selective capacity to inhibit in the cytotoxic assay and to bind, when labelled with 125I, to 2 specific antisera. The active fractions isolated from pleural effusions fully crossreacted with fractions prepared from squamous-cell carcinoma extracts. CEA and bacterial antigens were not detected in the material, and the presence of alpha-fetoprotein, HLA and blood-group antigens may be ruled out on account of their respective molecular weights.
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- Clemetson K. J., Bertschmann M., Widmer S., Lüscher E. F. Water-soluble P-815 mastocytoma membrane antigens: separation of tumour-associated antigens from histocompatibility antigens. Immunochemistry. 1976 May;13(5):383–388. doi: 10.1016/0019-2791(76)90372-4. [DOI] [PubMed] [Google Scholar]
- Frost M. J., Rogers G. T., Bagshawe K. D. Extraction and preliminary characterization of a human bronchogenic carcinoma antigen. Br J Cancer. 1975 Apr;31(4):379–386. doi: 10.1038/bjc.1975.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Granlund D. J., Ritts R. E., Jr Soluble proteins of human bronchogenic carcinomas. Mayo Clin Proc. 1976 Jan;51(1):19–27. [PubMed] [Google Scholar]
- Hunter W. M. Radioimmunoassay and saturation analysis. Preparation and assessment of radioactive tracers. Br Med Bull. 1974 Jan;30(1):18–23. doi: 10.1093/oxfordjournals.bmb.a071161. [DOI] [PubMed] [Google Scholar]
- Kelly B., Levy J. G. Evidence for a common tumour-associated antigen in extracts of human bronchogenic carcinoma. Br J Cancer. 1977 Jun;35(6):828–833. doi: 10.1038/bjc.1977.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCoy J. L., Jerome L. F., Cannon G. B., Weese J. L., Herberman R. B. Reactivity of lung cancer patients in leukocyte migration inhibition assays to 3-M potassium chloride extracts of fresh tumor and tissue-cultured cells derived from lung cancer. J Natl Cancer Inst. 1977 Nov;59(5):1413–1418. doi: 10.1093/jnci/59.5.1413. [DOI] [PubMed] [Google Scholar]
- Tillack T. W., Rosai J., Vervynck D. J. Immunologic studies of glycoproteins isolated from cell membranes of human lung carcinomas. J Natl Cancer Inst. 1974 Apr;52(4):1059–1067. doi: 10.1093/jnci/52.4.1059. [DOI] [PubMed] [Google Scholar]
- Veltri R. W., Mengoli H. F., Maxim P. E., Westfall S., Gopo J. M., Hauang C. W., Sprinkle P. M. Isolation and identification of human lung tumor-associated antigens. Cancer Res. 1977 May;37(5):1313–1322. [PubMed] [Google Scholar]
- Wolf A., Steele K. A., Alexander P. Estimation in sera by radioimmunoassay of a specific membrane antigen associated with a murine lymphoma. Br J Cancer. 1976 Feb;33(2):144–153. doi: 10.1038/bjc.1976.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolf A., Steele K. A. Separation of a tumour specific transplantation-type antigen from the ascitic fluid of mice bearing a syngeneic lymphoma. Br J Cancer. 1975 Jun;31(6):684–688. doi: 10.1038/bjc.1975.116. [DOI] [PMC free article] [PubMed] [Google Scholar]