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. 1978 Jun;37(6):965–973. doi: 10.1038/bjc.1978.141

Squamous Lesions in the Lungs of Rats Exposed to Tobacco-smoke-condensate Fractions by Repeated Intratracheal Instillation

P J Simons, P N Lee, F J C Roe
PMCID: PMC2009662  PMID: 678436

Abstract

Twice-weekly intratracheal instillations in rats of up to 24 mg of Fraction (R + P)G suspended in either infusine (I) or buffered saline/gelatine (BS/G) gave rise to foci of squamous metaplasia of alveolar epithelium (SqM) and squamous neoplasms (SqN). Fraction (R + P)G, which is a fraction of cigarette-smoke condensate almost as tumorigenic for mouse skin as the nearly 30 × larger mass of condensate from which it is derived, could be given in this way for up to 40 weeks without excessive mortality or any marked effect on the rate of body-weight gain. By contrast, similar treatment with Fraction N(QG), a fraction having very low tumorigenic activity for mouse skin, induced no SqN and barely any excess of SqM over that induced by either vehicle alone.

The effects of Fraction (R + P)G on the incidence of SqM and SqN were both time and dose related, the effect on SqM incidence being already evident after 10 weeks of treatment. No SqN seen were unequivocally malignant, though, due to the design of the experiment, only 5 rats exposed to Fraction (R + P)G were observed more than 60 weeks after the start of the experiment.

Other changes in the lung, including aggregates of alveolar macrophages laden with golden-brown pigment (GBM) and foci of cuboidal/columnar metaplasia of alveolar epithelium (CCM), were frequently seen in response to both fractions. Fraction (R + P)G administered in I was more effective in causing SqM and SqN than the same fraction administered in BS/G. The implications of the findings are discussed, particularly the possibility that the intratracheal/instillation technique might be useful as a rapid bioassay for comparing the tumorigenicity of different cigarette-smoke condensates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernfeld P., Homburger F., Russfield A. B. Strain differences in the response of inbred Syrian hamsters to cigarette smoke inhalation. J Natl Cancer Inst. 1974 Oct;53(4):1141–1157. doi: 10.1093/jnci/53.4.1141. [DOI] [PubMed] [Google Scholar]
  2. Davies R. F., Day T. D. A study of the comparative carcinogenicity of cigarette and cigar smoke condensate on mouse skin. Br J Cancer. 1969 Jun;23(2):363–368. doi: 10.1038/bjc.1969.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davis B. R., Whitehead J. K., Gill M. E., Lee P. M., Butterworth A. D., Roe F. J. Response of rat lung to tobacco smoke condensate or fractions derived from it administered repeatedly by intratracheal instillation. Br J Cancer. 1975 Apr;31(4):453–461. doi: 10.1038/bjc.1975.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davis B. R., Whitehead J. K., Gill M. E., Lee P. N., Butterworth A. D., Roe F. J. Response of rat lung to 3,4-benzpyrene administered by intratracheal instillation in infusine with or without carbon black. Br J Cancer. 1975 Apr;31(4):443–452. doi: 10.1038/bjc.1975.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davis B. R., Whitehead J. K., Gill M. E., Lee P. N., Butterworth A. D., Roe F. J. Response of rat lung to inhaled tobacco smoke with or without prior exposure to 3,4-benzpyrene (BP) given by intratracheal instillation. Br J Cancer. 1975 Apr;31(4):469–484. doi: 10.1038/bjc.1975.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Day T. D. Carcinogenic action of cigarette smoke condensate on mouse skin. Br J Cancer. 1967 Mar;21(1):56–81. doi: 10.1038/bjc.1967.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dontenwill W., Chevalier H. J., Harke H. P., Lafrenz U., Reckzeh G., Schneider B. Investigations on the effects of chronic cigarette-smoke inhalation in Syrian golden hamsters. J Natl Cancer Inst. 1973 Dec;51(6):1781–1832. doi: 10.1093/jnci/51.6.1781. [DOI] [PubMed] [Google Scholar]
  8. Lee P. N., Rothwell K., Whitehead J. K. Fractionation of mouse skin carcinogens in cagarette smoke condensate. Br J Cancer. 1977 Jun;35(6):730–742. doi: 10.1038/bjc.1977.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Peto R. Editorial: Guidelines on the analysis of tumour rates and death rates in experimental animals. Br J Cancer. 1974 Feb;29(2):101–105. doi: 10.1038/bjc.1974.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SHABAD L. M. Experimental cancer of the lung. J Natl Cancer Inst. 1962 Jun;28:1305–1332. [PubMed] [Google Scholar]
  11. Schreiber H., Nettesheim P., Martin D. H. Rapid development of bronchiolo-alveolar squamous cell tumors in rats after intratracheal injection of 3-methylcholanthrene. J Natl Cancer Inst. 1972 Aug;49(2):541–554. [PubMed] [Google Scholar]
  12. Whitehead J. K., Rothwell K. The mouse skin carcinogenicity of cigarette smoke condensate: fractionated by solvent partition methods. Br J Cancer. 1969 Dec;23(4):840–857. doi: 10.1038/bjc.1969.104. [DOI] [PMC free article] [PubMed] [Google Scholar]

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